| Project/Area Number |
01571202
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
MIURA Yoshiharu Osaka University, Pharmaceutical Sciences, Professor, 薬学部, 教授 (50028825)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Kazumasa Osaka University, Pharmaceutical Sciences, Research Associate, 薬学部, 助手 (30199062)
MIYAMOTO Kazuhisa Osaka University, Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (30028849)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Catharanthus roseus / Indole alkaloid / Multiple shoot culture / Phytohormone / Light irradiation / Liquid culture / アルカロイド / 近紫外光 |
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| Research Abstract |
The results obtained in this study were as follows :
1)The contents of alkaloids and growth of multiple shoot cultures were affected by phytohormones and light irradiation. Especially, near-ultraviolet light specifically stimulated the production of dimeric antitumor alkaloids such as vinblastine and leurosine.
2)Vindoline and catharanthine were efficiently produced by a two-step cultivation method, in which growth of the cultures was stimulated on the first-step and alkaloid production was stimulated on the second-step. Dimeric alkaloids production was also stimulated by a three-step cultivation method, in which the cultivation under near-ultraviolet light was added to above two-step one as the third-step for stimulation of dimeric alkaloid production.
3)A non-enzymatic coupling of vindoline and catharanthine to synthesize dimeric alkaloids specifically ocurred under near-ultraviolet irradiation in the presence of flavin mononucleotide. Vinblastine could be produced from the iminium intermediate synthesized by above couplin in the presence of a crude enzyme extract from plant leaves.
4)A liquid-surface culture was available for cultivation of multiple shoot cultures using liquid medium.
In the future, further investigations on the regulation mechanism of dimeric alkaloids and scale-up of the cultivation are necessary for establishment of efficient alkaloid production system using multiple shoot cultures of Catharanthus roseus.
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