| Project/Area Number |
01571233
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Fac of Pharmaceutical Sciences, Nagoya City University |
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Principal Investigator |
ONOZAKI Kikuo Dep of Hygienic Chemistry, Fac of Pharmaceutical Sciences, Nagoya City University, Professor, 薬学部, 教授 (20101313)
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| Co-Investigator(Kenkyū-buntansha) |
笠原 忠 自治医科大学, 医動物免疫学, 助教授 (60049096)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | interleukin 1 / cytokine / growth regulation / myeloma cell / myeloid leukemia cell / cell cycle / signal transduction / amniotic fluid / 増殖分化 / プロスタグランジンE_2 / インタ-ロイキン6 |
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| Research Abstract |
Interleukin 1 (IL-1) which is produced by macrophages plays an important role in various host reactions. In this study we we investigated the signal transduction pathway of IL-1 antiproliferative and differentiative activities.
1. IL-1 antiproliferative effect in relation to cell cycle. IL-1 inhibits the growth of a human myeloma cell line A375-6. IL-1 antiproliferative effect appeared to be resulted from the prevention of cell movement from G1 to S phase.
2. IL-1 antiproliferative and differentiative effects and signal transduction pathway. IL-1 inhibits the growth of a mouse myeloid leukemic cell line (M1), and in conjunction with other cytokines IL-1 induces M1 cell differentiation into macrophages. Prostaglandin E2, Ca^<2+> and PKC did not mediate IL-1 effect. In contrast, cAMP exhibited the effect similar to that of IL-1. However, IL-1 failed to increase intracellular cAMP levels in M1 cells. IL-1 also failed to increase cAMP in other cell lines. Therefore, it was suggested that IL-1 provides similar signal as cAMP without increasing intracellular cAMP level.
3. Changes in IL-1 levels in human amniotic fluid (AF) with gestational ages and delivery. Since IL-1 regulates growth and differentiation of many cell types, we measured the IL-1 levels in human AFs which form the intrauterine environment for fetus. IL-1alpha was present in AF of early gestational ages 24 weeks, and it increased with gestational ages 36 weeks. In contrast, ILー1beta was not detectable. However, the content of IL-1alpha and beta in AF obtained transvaginally during delivery with labor was extremely high. Therefore, IL-1alpha may relate to fetal growth. IL-1 alpha and beta also may play an important role in occurrence of labor.
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