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Platelet-mediated cytotoxicity

Research Project

Project/Area Number 01571242
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionEhime College of Health Science

Principal Investigator

OKADA Mariko  Ehime College of Health Science, Department of Clinical Pathology, Associate Professor, 臨床検査学科, 助教授 (60111118)

Co-Investigator(Kenkyū-buntansha) TOMINAGA Akio  Ehime College of Health Science, Department of Clinical Pathology, Associate Pro, 臨床検査学科, 助教授 (90036450)
SAGAWA Terutaka  Ehime College of Health Science, Department of Clinical Pathology, Assistant Pro, 臨床検査学科, 助手 (90162320)
伊藤 晃  愛媛県立医療技術短期大学, 臨床検査学科, 助手 (30203128)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
KeywordsPlatelets / Cytotoxic effect / Hemolysis / Leukemia / Protease inhibitor / 腫瘍細胞瘍害反応 / トロンビン活性化 / 透過型電顕 / 免疫電顕 / GMPー140 / 血小板依存性細胞傷害反応 / 腫瘍細胞株 / 自己赤血球溶血反応 / cAMP / シクロオキシゲナ-ゼ阻害剤 / 電子顕微鏡観察 / 活性化血小板 / 赤血球形態変化 / ガン細胞傷害反応 / エステラ-ゼ
Research Abstract

1) Gel-filtered human platelets (PL) exerted lytic activity on autologous red blood cells (RBC) when they were co-incubated at 37゚C with PL-activating agents in the absence of plasma. Lysis of activated PL themselves did not occur during the incubation period examined. Morphological observations showed that RBC exposed to thrombin-activated PL were fragmented and/or transformed into spherocytes. When PL and RBC were prevented from coming into direct cell to cell contact by means of a membrane barrier, their cytotoxicity was reduced but not eliminated completely. The cytotoxic activity was carried by activated PL which had lost most of the contents in granules, but not by PLfree supernatants obtained after activation of PL, and the activity was energy-dependent and cAMP-regulated. These observations suggested that the cytotoxic activity was carried by some diffusible and easily inactivated factors, which were continuously produced and liberated from activated PL. These results provide the first evidence for a direct role of activated PL in mediation of RBC-damage in the absence of any plasma factors.
2) Gel-filtered PL exerted cytotoxicity against K-562 cells, a chronic myelogenic leukemia cell line, when incubated in serumfree medium for several hours at 37゚C, a condition where no killing of normal peripheral lymphocytes occurred. Some protease inhibitors inhibited the cytotoxicity of PL. Ultrastructural observations showed that PL induced cytotoxic changes in target K-562 cells, including swelling of the cell, marginal clumping of nuclear chromatin, dilation of mitochondoria, and ruprure of the cytoplasmic membrane. These results suggest that PL may be involved in host defense against neoplasia and that certain proteases are implicated in the cytotoxic effect of PL.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report

Research Products

(21 results)

All Other

All Publications (21 results)

  • [Publications] Sagawa,T.: "Human platelets effectively kill K-562 cells,a chronic myelogenic leukemia cell line,in vitro." Jpn.J.Cencer Res.81. 449-453 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐川 輝高: "Kー562に対する血小板細胞傷害性におけるプロテア-ゼインヒビタ-の効果." 愛媛県立医療技術短期大学紀要. 3. 99-106 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岡田 真理子: "血小板による腫瘍細胞傷害反応の電顕的観察" 愛媛県立医療技術短期大学紀要. 4. 61-65 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okada,M.: "Cytotoxicity of activated platelets to autologous red blood cells."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sagawa, T., et al.: "Human platelets effectively kill K-562 cells, a chronic mtelogenic leukemia cell line, in vitro." Jpn. J. Cancer Res.81(5). 449-453 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Segawa, T., et al.: "Effects of protease inhibitors on platelet mediated-cytotoxity against K-562 cells." Bulletin of Ehime College of Health Science. 3. 99-106 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okada, M., et al.: "Ultrastructure of effectortarget cell interaction in platelet-mediated cytotoxic reaction." Bulletin of Ehime College of Health Science. 4. 61-65 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okada, M., et al.: "Cytotoxicity of activated platelets to autologous red blood cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岡田 真理子: "血小板による腫瘍細胞傷害反応の電顕的観察" 愛媛県立医療技術短期大学紀要. 4. 61-65 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okada,M.: "Cytotoxocity of activated platelets to autologous red blood cells."

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Sagawa: "Human Platelets Effectively Kill Kー562 Cells,a Chronic Myelogenic Leukemic Cell Line,in vitro." Jpn.J.Cancer Res.81. 449-453 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐川 輝高: "ヒト血小板癌細胞傷害機能におけるプロテア-ゼインヒビタ-の効果" 日本癌学会総会記事. 49. 321 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐川 輝高: "ヒト血小板の腫瘍細胞株に対するキラ-活性" 日本免疫学会総会・学術集会記録. 20. 306 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岡田 真理子: "生体防御における血小板の役割ー抗腫瘍エフェクタ-機能について" 日本細菌学雑誌. 46. 308 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岡田 真理子: "ヒト血小板による腫瘍細胞傷害反応の電顕的観察" 日本血液学会雑誌. 54. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kodama: "Lysis of Autologous Erythrocytes by Human Platelets." 7th International Congress of Immunology,Abstracts. 499 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐川輝高: "血小板の白血球細胞株Kー562に対する細胞傷害" 日本免疫学会総会・学術集会記録. 19. 338 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 岡田真理子: "血小板依存性溶血反応の機序について" 日本免疫学会総会・学術集会記録. 19. 339 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 岡田真理子: "活性化血小板による赤血球の形態変化と変形能低下" 日本血液学会雑誌. 53. 260 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐川輝高: "ヒト血小板による細胞傷害" 日本細菌学雑誌. 45. 417 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Sagawa: "Human Platelets Effectively Kill Kー562 Cells,a Chronic Myelogenic Leukemia Cell Line,in vitro." Jpn.J.Cancer Res.81. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-03-31   Modified: 2016-04-21  

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