| Project/Area Number |
01580163
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Osaka University |
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Principal Investigator |
HIRAKI Yuji (Osaka University, Faculty of Dentistry, Dept. of Biochemistry, Instructor), 歯学部, 助手 (40144498)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Fujio (Osaka University, Faculty of Dentistry, Dept. of Biochemistry, Professor), 歯学部, 教授 (40028717)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Cartilage-derived factor / chondrocyte / chondromodulin-I / cell differentiation / cell growth / fibroblast growth factor / extracellular matrix / endochondral bone formation / cDNAクロ-ニング / 精製 / アミノ酸配列 / プロテオグリカン合成 |
|---|
| Research Abstract |
Besides known hormones and growth factors, some functional matrix components have been suspected to play important roles in the control of chondrogenic expression. Here I report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I, ChM-I) as a tissue-specific functional matrix component identified and cloned for the fist time. ChM-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic Fibroblast Growth Factor (FGF). Bovine ChM-I cDNA revealed that mature Ch-M-I consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of ChM-I mRNA was observed only in cartilage.
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