• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and Function of the Core Protein of Fibronectin-binding Proteoglycan

Research Project

Project/Area Number 01580169
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionThe University of Shizuoka

Principal Investigator

ISEMURA Mamoru  The University of Shizuoka, School of Food and Nutritional Sciences, Professor, 食品栄養科学部, 教授 (40028197)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsplacenta / proteoglycan / fibronectin / cell adhesion / cell spreading / extracellular matrix / cancer / basement membrane / プロテオグリカン / 腎炎
Research Abstract

Followings are new findings from the studies on a fibronectin-binding proteoglycan which we have newly found in the human term placenta. 1. The study of the interaction between the proteoglycan and the thermolysin fragments of fibronectin has indicated that the proteoglycan binds fibronectin via a gelatin-binding domain. 2. The proteoglycan has no activity promoting adhesion and spreading of FL amnion cells on plastic dishes, but it does promote cell-spreading of these cells, if the proteoglycan has been bound to the fibronectin-coated dishes. These cells show good adhesion onto the fibronectin-coated dishes, but they do not spread well without the proteolycan bound to fibronectin. These data suggest that the cell activities can be affected differently by a combination of extracellular matrix components. 3. Immunohistochemical studies on basement membrane components including the proteoglycan of squamous cell carcinoma have suggested that poorly differentiated tumors tend to have highly degraded basement membranes which may reflect a greater proteolytic activity than that in the well differentiated tumors. In some cases, the proteoglycan is detectable in the tumor stroma in association with fibronectin, suggesting that the binding between fibronectin and the proteoglycan does occur in vivo. The determination of the core protein structure by the analysis of cDNA would be useful to elucidate the biological function of this proteoglycan.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] M.Takamoto: "Structures of asparagineーlinked oligosaccharide of human placental fibronectin." Journal of Biochemistry. 105. 742-750 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takamoto: "Detection of bisected biantennary form in the asparagineーlinked oligosaccharides of fibronectin isolated from human term aminotic fluid." Journal of Biochemistry. 106. 228-235 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kazama: "Immunohistochemical localization of heparan sulfatecontaining proteoglycan in normal human skin with monoclonal antibodies:comparison with that of fibronectin." Archives of Dermatological Research. 281. 440-442 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A.Oomura: "Alterations in the extracellular matrices in human glomerular diseases." Virchows Archiv A. 415. 151-159 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Oguro: "Immunohistochemical alterations in basement membrane components of squamous cell carcinoma." Journal of Investigative Dermatology. 96. 250-254 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kazama: "Immunohistochemical alterations of basement membrane components in carcinomas with eccrine differentiation."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 高橋 達: "肝細胞外マトリックス フィブロネクチン" 肝胆膵. 18. 379-387 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "細胞接着とインテグンス-パ-ファミリ-" ファルマシア. 26. 827-831 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "神経組織のフィブロネクチン" 細胞. 22. 336-339 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "細胞のがん化、転移と細胞外マトリックス" BIO medica. 5. 1213-1216 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "細胞外マトリックス Fibronectin" 肝胆膵.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "レセプタ-.細胞外マトリックスのバイオサイエンスとバイオテクノロジ-" アイピ-シ-出版部, 11 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "新生化学実験講座第3巻糖質II" 東京化学同人,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "生物薬科学実験講座Iー4糖質III巻" 東京化学同人,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊勢村 護: "新生化学実験講座第3巻糖質I糖タンパク質(上)" 東京化学同人, 5 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takamoto, et al: "Structures of asparaginelinked oligosaccharides of human placental fibronectin." Journal of Biochemistry. Vol. 105. 742-750 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takamoto, et al.: "Detection of bisected biantennary form in the asparagine-linked oligosaccharides of fibronectin isolated from human term amniotic fluid." Journal of Biochemistry. Vol. 106. 228-235 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kazama, et al.: "Immunohistochemical localization on heparan sulfate-containing proteoglycan in normal human skin with monoclonal antibodies : comparison with that of fibronectin." Archives of Dermatological Research. Vol. 281. 440-442 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A. Oomura, et al.: "Alterations in the extracellular matrices in human glomerular diseases." Virchows Archiv A. Vol. 415. 151-159 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Oguro, et al.: "Immunohistochemical alterations in basement membrane components of squamous cell carcinoma." Journal of Investigative Dermatology. Vol. 96. 250-254 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Takahashi, et al.: "Fibronectin and liver diseases." Kan. Tan. Sui(Japan). Vol. 18. 379-387 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Isemura: "Cell adhesion and integrin superfamily." Farumashia. Vol. 26. 827-831 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Isemura: "Fibronectin in the nervous tissue." The Cell. Vol. 22. 336-339 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Isemura, et al.: "Extracellular matrix in malignant transformation of cells and in metastasis of tumor cells." Biomedica. Vol. 5. 1213-1216 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Isemura, et al.: "Extracellular matrix fibronectin in liver diseases." Kan. Tan. Sui.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takamoto: "Structures of asparagineーlinked oligosaccharide of human placentalfibronectin." Journal of Biochemistry. 105. 742-750 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Takamoto: "Detection of bisected biantennary from in the asparagineーlinked oligosaccharides of fibronectin isolated from human term aminotic fluid." Journal of Biochemistry. 106. 228-235 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kazama: "Immunohistochemical localization of heparan sulfateーcontaining proteoglycan in normal human skin with monoclonal antibodies:comparison with that of fibronectin." Archives of Dermatological Research. 281. 440-442 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Oomura: "Alterations in the extracellular matrices in human glomerular diseases." Virchows Archiv A. 415. 151-159 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Oguro: "Immunohistochemical alterations in basement membrane components of squamous cell carcinoma." Journal of Investigative Dermatology. 96. 250-254 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kazama: "Immunohistochemical alterations of basement membrane components in carcinomas with eccrine differentiation."

    • Related Report
      1990 Annual Research Report
  • [Publications] 高橋 達: "肝細胞外マトリックスフィブロネクチン" 肝胆膵. 18. 379-387 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "細胞接着とインテグンス-パ-ファミリ-" ファルマシア. 26. 827-831 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "神経組織のフィブロネクチン" 細胞. 22. 336-339 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "細胞のがん化、転移と細胞外マトリックス" BIO medica. 5. 25-28 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "細胞外マトリックス Fibronectin" 肝胆膵.

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "レセプタ-・細胞外マトリックスのバイオサイエンスとバイオテクノロジ-" アイピ-シ-出版部, 11 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "新生化学実験講座第3巻糖質II" 東京化学同人,

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "生物薬科学実験講座Iー4糖質III巻" 東京化学同人,

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊勢村 護: "新生化学実験講座第3巻糖質I糖タンパク質(上)" 東京化学同人, 5 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masaya Takamoto: "Structures of asparagine-linked oligosaccharides of human placental fibronectin" Journal of Biochemistry. 105. 742-750 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masaya Takamoto: "Detection of bisected biantennary from in the as paragine-linked oligosaccharides of fibronectin isolated from human term amniotic fluid" Journal of Biochemistry. 106. 228-235 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Asuko Oomura: "Alterations in the extracellular matrix components in human glomerular diseases" Virchows Archiv A. 415. 151-159 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takashi Kazama: "Immunohistochemical localization of heparan sulfatecontaining proteoglycan in normal human skin with mono-clonal antibodies: comaprison with that of fibronectin" Atchives of Dermatological Research. 281. 440-442 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Keiko Oguro: "Immunohistochemical alterations in basement membrane components of squamous cell carcinoma"

    • Related Report
      1989 Annual Research Report
  • [Publications] 伊勢村護: "細胞接着とイングリンス-パ-ファミリ-" ファルマシア.

    • Related Report
      1989 Annual Research Report
  • [Publications] 伊勢村護: "細胞外マトリックスのバイオサイエンスとバイオテクノロジ-" アイピ-シ-,

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi