| Project/Area Number |
01580176
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
物質生物化学
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ITOH Nobuyuki Kyoto University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10110610)
|
|---|
| Project Period (FY) |
1989 – 1990
|
| Project Status |
Completed (Fiscal Year 1990)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Neurotransmitter / Gene / Drosophila / アセチルコリン / 遺伝子構造 |
|---|
| Research Abstract |
Choline acetyltransferase is the biosynthetic enzyme for the neurotransmitter acetylcholine. By screening Drosophila cDNA library with a mixture of three different monoclonal antibodies to Drosophila choline acetyltransferase, we isolated Drosophila choline acetyltransferase cDNA. The cDNA insert consisted of a coding region 2,190 nucleotides long and a 3' -noncoding region 284 nucleotides long. Northern analysis of Drosophila head mRNA with the cDNA insert as a probe showed the choline acetyltransferase mRNA to be approximately 4,700 nucleotides long. We compared the amino acid sequences of Drosophila choline acetyltransferase and Torpedo acetylcholinesterase and found a global homology of 21-44% identity between these proteins. Six homologous segments of 40-60 amino acids cover 38% and 54% of the sequences, raising the possibility of a common evolutionary origin. We also isolated and characterized the cDNA related with Drosophia choline acetyltransferase.
|
