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Studies on the possible roles for O^-_ and H_2 O_2 on the stimulation of sugar transport in mouse fibroblasts

Research Project

Project/Area Number 01580198
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

IWASHIMA Akio  Kyoto Prefectural University of Medicine Department of Biochemistry, Professor, 医学部, 教授 (70079705)

Co-Investigator(Kenkyū-buntansha) NISHINO Hoyoku  Kyoto Prefectural University of Medicine Department of Biochemistry, Associate P, 医学部, 助教授 (10079709)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsSugar Transport System / Hydrogen Peroxide / Superoxide / Na^+ / H^+ Exchanger / Tumor Promoter / Insulin / 2-Deoxyglucose / H^+交換体 / 2-デオキシグルコ-ス
Research Abstract

Xanthine/xanthine oxidase and H_2O_2 stimulated sugar transport in mouse fibroblasts. Application of superoxide dismutase and catalase to the cells showed an inhibitory effect on these agent-stimulated sugar transports. Addition of amiloride and 4-acetamide-4'isothiocyanostilbene-2, 2'-disulfonic acid(SITS), which abolish the cytoplasmic alkalinization, inhibited the stimulation of sugar transport by xanthine/xanthine oxidase in the presence of catalase. The calmodulin antagonists, N-(6-aminohexyl)-5-chloro1-naphthalenesulfonamide(W-7)and trifluoperazine inhibited H_2O_2 -stimulated sugar transport.
These results suggest that O^-_ stimulates sugar transport in an intracellular pH-dependent manner and that H_2O_2 stimulates sugar transport in a calcium-calmodulin-dependent manner. These mechanisms may be involved in sugar-transport stimulation in mouse fibroblast BALB/3T3 cells by the tumor-promoting phorbol ester phorbol-12, 13-dibutyrate and insulin, since the stimulatory effects of these agents were inhibited by scavengers of oxygen radicals.
Our previous studies showed that phorbol-12, 13-dibutyrate or insulin-stimulated sugar transport in mouse fibroblast Swiss 3T3 cells is regulated at least three different mechanisms ; i)calcium-calmodulin-dependent regulation, ii)intracellular ph-dependent regulation, and iii)calcium-activated phospholipid-dependent protein kinase-dependent regulation. These mechanisms lead to the translocation of sugar transporters from an intracellular sites to the plasma membrane ; this process results in an increase in the rate of sugar transport. Combining these previous observations with the present results, we have concluded that the calcium-calmodulin-dependent process in H_2O_2 -stimulated sugar transport system and intracellular ph-dependent process in O^-_ -stimulated sugar transport system may be involved in the essential steps for the stimulation of sugar transport in mouse fibroblast cells by phorbol-12, 13-dibutyrate and insulin.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ichiro Honda: "Inhibitory effects of 3-nitro2,4,6-trihydroxybenzamides on Epstein-Barr virus early antigen induction" Cancer Letters. 59. 83-88 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuhiro Irie: "Quantitative structure-activity studies on indole alkaloid tumor promoter indolactam congeners" Carcinogenesis. 12. 1883-1886 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuhiro Irie: "Biological activities and cellular uptake studies of fluorescent derivatives of indole alkaloid tumor promoter teleocidin" Int. J. Cancer. 43. 513-519 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Rundi Ma: "Influence of hepatoma transplantation on lipoperoxidation of liver, spleen, heart, and red blood cell membrane" J. Kyoto Pref. Univ. Med.98. 835-840 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junko Takayasu: "Anti-tumor-promoting activity of derivatives of abieslactone, a natural triterpenoid isolated from several Abies genus" Cancer Letters. 53. 141-144 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toru Okuyama: "Studies on the antitumor-promoting activity of naturally occurring substances. III. Structure of a new coumarin and anti-tumor-promoter activity of coumarins from Angelicae Radix" Shouakugaku Zasshi. 44. 346-348 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ichiro Honda: "Inhibitory effects of 3-nitro-2, 4, 6-trihydroxybenzamides on Epstein-Barrvirus early antigen induction" Cancer Letters. 59. 83-88 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuhiro Irie: "Quatitative structure-activity studies on indole alkaloid tumor promoter indolactam congeners" Carcinogenesis. 12. 1883-1886 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ichiro Honda: "Inhibitory effects of 3ーnitroー2,4,6ーtrihydroxybenzamides on EpsteinーBarr virus early antigen induction" Cancer Letters. 59. 83-88 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuhiro Irie: "Quantitative structureーactivity studies on indole alkaloid tumor promoter indolactam congeners" Carcinogenesis. 12. 1883-1886 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Junko Takayasu: "Antiーtrmorーpromoting activity of derivatives of abieslactone, a natural triterpenoid isolated from several Abies genus" Cancer Letters. 53. 141-144 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toru Okuyama: "Studies on the antitumorーpromoting activity of naturally occurring substances.III.Structure of a new 8oumarin and antiーtumorーpromoter activity of coumarins from Angelicae Radix" Shoyakugaku Zasshi. 44. 346-348 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuhiro Irie: "Biological activities and cellular uptake studies of fluorescent derivatives of indole alkaloid tumor promoter teleocidin" Int.J.Cancer. 43. 513-519 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Rundi Ma: "Influence of hepatoma transplantation on lipoperoxidation of liver,spleen,heart,and red blood cell membrane" J.Kyoto Pref.Univ.Med.98. 835-840 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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