| Project/Area Number |
01580205
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
FUJISAWA Atsuko NCNP. Morecular Genetics., 神経研究所・遺伝子工学部, 室長 (60209038)
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| Co-Investigator(Kenkyū-buntansha) |
NABESHIMA Yoichi NCNP, Moleular Grenetics., 神経研究所・遺伝子工学部, 部長 (60108024)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Molecular Biology / Developmental Biology / Differentiation / Muscle Development / Gene Regulation |
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| Research Abstract |
Using mouse MyoD and myogenin cDNAs as probes, we have isolated their chicken homologues. Polypeptide sequence of our chicken MyoD was found to be identical with that reported previously (CMD1 ; Lin et al., 1989) except several amino acid residues. Chicken myogenin cDNA encodes a polypeptide of 227 amino acids the sequence of which is highly homologous to that of mouse myogenin. When these cDNAs were expressed in C3H10T1/2 cells, the cells were converted to myoblasts, which subsequently gave rise to myotubes. Furthermore, transient expression of these cDNAs in chicken primary fibroblasts activated the promoter of the chicken LCI gene (a skeletal myosin alkaine light chain gene) fused with chloramphenicol acetyltransferas gene. The activation of the gene was found to be dependent on the existence of the enhancer which is localized 2 kb upstream of the LC1 gene. This enhancer contains two consensus sequences for Myod binding sites which are adjacent to each other, and the disruption of either of the MyoD binding sites dramatically reduced the enhancer activity. These results suggest that some myogenic factors including MyoD and myogenin are involved in the activation of the LC1 gene by direct binding to its enhancer during muscle development.
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