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Analysis of Radiation Hypersensitivity of Human Cells From Genetic Disease by the use of Chromosome Transfer

Research Project

Project/Area Number 01580209
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionKyoto University

Principal Investigator

EJIMA Yosuke  Kyoto University, Radiation Biology Center, Research Associate, 放射線生物研究センター, 助手 (50127057)

Co-Investigator(Kenkyū-buntansha) SASAKI Masao  Kyoto University, Radiation Biology Center, Professor, 放射線生物研究センター, 教授 (20013857)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsRadiosensitivity / Human genetic disease / Chromosome transfer
Research Abstract

The chromosomal localization of the gene which complements radiation hypersensitivity of AT(ataxia telangiectasia)cells was studied by microcell-mediated chromosome transfer.
1. An immortalized AT cell line, AT2KYSV, aDd its 6-thioguanineresistant derivative, AT2KYSVTG, were used as recipients. Five strains of mouse A9 cells carrying a single human chromosome were used as chromosome donors. The chromosomes carried were PSV2neo-tagged human chromosome 11, X chromosome, or X/11 recombinant chromosomes containing either llpter-ql3, llpter-q23 or llpll-qter.
2. Among seven G418-resistant microcell hybrids obtained from the transfer of a PSV2neo-tagged chromosome 11, three clones showed restoration of radiation resistance with concomitant gain of an extra chromosome 11, while the others contained no recognizable or deleted chromosome 11.
3. The HAT-resistant microcell hybrids obtained from the transfer of an intact X chromosome or an X/11 chromosome bearing the Ilpter-ql3 region did not show a diffprence in radiosensitivity from parental AT cells, while those obtained from the transfer of X/11 chromosomes bearing either the llpll-qter or Ilpter-q23 region exhibited a marked radioresistance.
4. A HAT-resistant but radiosensitive variant was further obtained from the microcell fusion with an A9 strain carrying an X/11 chromosome bearing llpll-qter region, in which a deletion at the llq23 region was found. The results indicate that the gene which complements a radiosensitive phenotype of AT is located at the q23 region of chromosome 11. The derivative chromosome 11 found here to be associated with concomitant loss of the AT locus or its function would be useful for the molecular dissection of the llq23 region with relevance to the AT gene.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 江島 洋介: "Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the assignment of radiosensitivity gene" International Journal of Radiation Biology. 58. 989-997 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 江島 洋介: "Determination of the chromosomal site for the human radiosensitive ataxia telangiectasia gene by chromosome transfer" Mutation Research. 250. 337-343 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] EJIMA, Yosuke: "Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the assignment of radiosensitivity gene" International Journal of Radiation Biology. 58. 989-997 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] EJIMA, Yosuke: "Determination of the chromosomal site for the human radiosensitive ataxia telangiectasia gene by chromosome transfer" Mutation Research. 250. 337-343 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 江島 洋介: "Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the assignment of radiosensitivity gene" International Journal of Radiation Biology. 58. 989-997 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 江島 洋介: "Determination of the chromosomal site for the human radiosensitive ataxia telangiectasia gene by chromosome transfer" Mutation Research. 250. 337-343 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 江島 洋介: "Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the assignment of radiosensitivity gene" Interanational Journal of Radiation Biology. 58. 989-997 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 江島 洋介: "Assignment of ataxia telangiectasia gene to chromosome region 11q23 by the use of microcellーmediated chromosome transfer" Human Genetics.

    • Related Report
      1990 Annual Research Report
  • [Publications] 江島洋介: "Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the assignment of radiosensitivity gene" International Journal of Radiation Biology.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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