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"Study on physical properties of inositol phospholipid in cellular signal transduction"

Research Project

Project/Area Number 01580262
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionNagoya University

Principal Investigator

OHKI Kazuo  Nagoya University Associate Professor Faculty of Engineering, 工学部, 助教授 (80115394)

Co-Investigator(Kenkyū-buntansha) 松岡 審爾  名古屋大学, 工学部, 助手 (90190420)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsinositol phospholipid / phosphatidic acid / calcium ion / Structure of biomembrane / lipid-protein interaction / signal transduction / physical property of membrane / phase separation / 静電相互作用 / 疎水相互作用
Research Abstract

Phosphatidic acids play an important role in cellular function ; for example, proliferation of a cell, signal transduction on plasma membranes and mobilization of calcium ions. In the present study, interactions of phosphatidic acid with poly (L-lysine) and calcium ions were investigated by differential scanning calorimetry and X-ray diffraction technique. Results on phosphatidic acid/poly (L-lysine) system indicate that poly (L-lysine) interacts with phosphatidic acid electrostatically without affecting the chain-packing of the lipid bilayer membranes and that the interaction does not obey the one-to-one sto ichiometry. Further analysis of X-ray diffraction data gives a probable model of phosphatidic acid/poly (L-lysine) membranes. Calorimetric study on phosphatidic acid/ (phosphatidylcholine and/or phosphatidylethanolamine) /Ca2+ system elucidates a fine structure of membranes composed of the mixed phospholipids. In these membranes, phosphatidic acid is not completely segregated by Ca2+ but studded in the membranes. The present study is helpful to proceed a research of relationship between membrane structure and function.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] 大木 和夫: "Ethanol induces interdigitated gel phase between lamellar gel phase and ripple phase in phosphatidylcholine membranes:a scanning density meter study" Biochimica et Biophysica Acta. 1028. 215-222 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大木 和夫: "Effect of substitution of hydrogen oxide by deuterium oxide on thermotropic transition between the interーdigitated gel phase of dihexadecylphosphatidylcholine" Biochemical and Biophysical Research Communications. 174. 102-106 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高橋 浩: "Electrostatic interaction of poly(Lーlysine)with dipalmitoylphosphatidic acid studied by Xーray diffraction" Biochimica et Biophysica Acta. 1069. 229-234 (1091)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大木 和夫: "リン脂質のInterdigitated構造" 生物物理. 31. 287-292 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Matko: "Luminescence quenching by nitroxide spin labels in aqueous solution:Studies on the mechanism of quenching" Biochemistry. 31. 703-711 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大木 和夫: "相原薫,上田眞太郎,野崎正彦 編集「バイオサ-ファクタント」" サイエンスフォ-ラム, 464 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大木 和夫: "日本生物物理の最前線」" 講談社ブル-バックス, 302 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大木 和夫: "野沢 義則・神野耕太郎・井上圭三・大木和夫編集「膜学実験シリ-ズ 第I部 生体膜編」" 共立出版,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuo Ohki, Kazuo Tamura and Ichiro Hatta: ""Ethanol induces interdigitated gel phase between lamellar gel phase and ripple phase in phosphatidylcholine membranes : a scanning density meter study"" Biochim. Biophys. Acta. 1028. 215-222 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuo Ohki: ""Effect of substitution of hydrogen>oxide by deuterium oxide on thermotropic transition>between the interdigitated gel phase of dihexadecylphosphatidylcholine"" Biochem. Biophys. Res. Commun.174. 102-106 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroshi Takahashi, Sinbzi Matsuoka, Satoru Kato, Kazuo Ohki and Ichiro Hatta: ""Electrostatic interaction of poly (L-lysine) with dipalmitoylphosphatidic acid studied by X-ray diffraction"" Biochim. Biophys. Acta. 1069. 229-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Janos Matko, Kazuo Ohki and Michael Edidin: ""Luminescence quenching by nitroxide spin labels in aqueous solution : Studies on the mechanism of quenching"" Biochemistry. 31. 703-711 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大木 和夫: "Ethanol induces interdigitafed gel phase between lamellar gel phase and ripple phase in phosphatidylcholine membranes:a scanning density meter study" Biochimica et Biophysica Acta. 1028. 215-222 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大木 和夫: "Effect of substitution of hydrogen oxide by deuterium oxide on thermotropic transition between the interdigitated gel phase of dihexadecylphosphatidylcholine" Biochemical and Biophysical Research Communications. 174. 102-106 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高橋 浩: "Electrostatic interaction of poly(Lーlysine)with dipalmitoylphosphatidic acid studied by Xーray diffraction" Biochimica et biophysica Acta. 1069. 229-234 (1091)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大木 和夫: "リン脂質のInterdigitated構造" 生物物理. 31. 287-292 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J. Matko: "Luminescence quenching by nitroxide spin labels in aqueous solution:Studies on the mechanism of quenching" Biochemistry. 31. 703-711 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 相原 薫,上田 眞太郎,野崎 正彦 編集,大木 和夫: "バイオサ-ファクタント" サイエンスファ-ラム, 464 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 日本生物物理学会編,大木 和夫: "生物物理学の最前線" 講談社ブル-バックス, 302 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野沢 義則,神野 耕太郎,井上 圭三,大木 和夫編集,大木 和夫: "膜学実験シリ-ズ 第I部 生体膜編" 共立出版,

    • Related Report
      1991 Annual Research Report
  • [Publications] 大木 和夫: "Ethanol induces interdigitated gel phase between lamellar gel phase and ripple phase in phosphatidylcholine membranes:a scanning density meter study" Biochimica at Biophysica Acta. 1028. 215-222 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大木 和夫: "Effect of substitution of hydrogen oxide by deuterium oxide on thermotropic transition between the interーdigitated gel phase of dihexadecylphosphatidylcholine" Biochemical and Biophysical Research Communications. 174. 102-106 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大木 和夫: "相原 薫、上田 眞太郎、野崎 正彦 編集「バイオサ-ファクタント」" サイエンスフォ-ラム, 464 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大木 和夫: "日本生物物理学会編「生物物理学の最前線」" 講談社ブル-バックス, 302 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大木和夫: "Short-and long-range Ca^<2+>-induced lateral phase separations in ternary mixtures of phosphatidic acid,phosphatidylcholine and phosphatidylethanolamine" Chemistry and Physics of Lipids. 50. 109-117 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大木和夫: "A region-matched hydrophobic interaction between melittin and dimyristoylphosphatidylcholine in a ternary mixture of phosphatidylcholines" Biochemical and Biophysical Research Communication. 164. 850-856 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大木和夫: "疎水相互作用と生体脂質膜構造" 表面. 27. 400-410 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大木和夫: "相原薫、上田眞太郎、野崎正彦 編集「バイオサ-ファクタント」" サイエンスフォ-ラム, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大木和夫: "日本生物物理学会編「生物物理学の最前線」" 講談社ブル-バックス, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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