| Project/Area Number |
01840028
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | University of Tokyo |
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Principal Investigator |
ISHIKAWA Hajime Univ.Tokyo, Fac.Sci., Professor, 理学部, 教授 (70012482)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAJI Munetochi Nihon Mektron Co., Ltd., Researcher, 研究員
YAMASHITA Tetsuro Univ.Tokyo, Fac.Sci., Instructor, 理学部, 助手 (20202377)
KAWAGUCHI Akihiko Univ.Tokyo, Coll.Arts.Sci., Professor, 教養学部, 教授 (80013332)
入野 信人 日本メクトロン株式会社, 研究主任
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1991: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1990: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Aphida / Enterobacteria / Antibiotic substance / Anti-fungal substance / Honeydew / Hyphal growth / Oligopeptide / Secondary Endosymbiont / ウリ類ツル枯病菌 / Staphylococcus |
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| Research Abstract |
1. Gram-negative bacteria were collected from the honeydew excreted by the pea aphids, Acyrthosiphon pisum which had been reared in the laboratory for a long period. The bacteria were culture in LB and the medium was determined for its antibiotic activity after 20fold concentration by lyophilization. The result showed that the medium did not have antibiotic activity against either E.coli or Bacillus subtilis while the honeydew itself, even without concentration, inhibited the growth of E.coli very effectively.
2. The cultured medium of the aphid enterobacteria was mixed with the potato dextrose medium for fungi, and determined for its anti-fungal activity in terms of hyphal growth retardation. As a result, it was shown that the aphid enterobacteria excreted a substance that inhibited the growth of several fungi specifically and effectively.
3. The distribution experiments with some organic solvents suggested that this effective substance was an oligopeptide with molecular weight of about 1,000.
4. There was no biochemical evidence that these enterobacteria were related to secondary endosymbionts that some aphid species harbor.
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