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Development of a System for Identification of Hybrid Plants

Research Project

Project/Area Number 01860002
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionNagoya University

Principal Investigator

HIRAI Atsushi  Nagoya U. Fac. Agr. Associate Professor, 農学部, 助教授 (60023470)

Co-Investigator(Kenkyū-buntansha) ITOH Kazuya  Oji Paper Co. Senior Scientist, 林木育種研究, 主任研究員
TAKABE Tetsuko  Nagoya U. Fac. Agr. Associate Professor, 農学部, 助教授 (60089852)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1989: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsCell fusion / Hybrid identification / Simple method for DNA isolation / Nonradioactive label / rRNA gene
Research Abstract

A simple and efficient method for identification of hybrids after cell fusion was established by combining a method for the efficient extraction of DNA from very small amounts of plant tissue and a Southern blotting with nonradioactively labeled rDNA from rice as a probe. One hundred milligrams of leaf or ca llus tissue, are sufficient for identification of the hybrid, and the amount of material can be reduced to as little as 10 mg. Since this method permits selection of parasexual hybrids not only at the plantlet stage but also at the callus stage, hybrids can be identified at the early stages of the development of the products of cell fusion.
Segments of mitochondrial DNA carrying the gene for the alphasubunit of ATPase (atpA) were detected by Southern hybridization with atpA from pea as probe. In the case of Nicotiana langsdorffii, we identified four fragments that are derived from combinations of two different 5' and two different 3' franking regions of atpA. All of the four types share the coding region, suggesting that the four types can be generated as a result of homologous recombination in the coding region of atpA. By contrast, N. Glauca generated only one fragment which indicated the existence of only a single type of atpA. In the case of somatic hybrids between N. Langsdorffii and N. Glauca, three new fragments were detected in addition to parental fragments. These new fragments can be explained by homologous recombination within the coding region of atpA. These results show that the coding region of atpA is involved not only in intragenomic homologous recombination but can also be involved in homologous recombination between two parental mitochondrial genomes of somatic hybrids.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 平井 篤志: "体細胞雑種植物の葉緑体ゲノムと植物体の再生" 細胞. 21. 220-223 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimada,H.: "A physical map and clone bank of the rice chloroplast genome." Plant Mol.Biol.Report.7. 284-291 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda,H.: "A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe." Jpn.J.Breeding. 40. 339-348 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Arakawa,K.: "Levels of betaine and betaine aldehyde dehydrogenase activity in the green leaves,and etiolated leaves and roots of barley." Plant Cell Physiol.31. 797-803 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 本田 秀夫: "非放射性DNAプロ-ブによる難種の検定" 植物細胞工学. 3. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda,H.: "The gene for the αーsubunit of ATPase: a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimada, H., R. F. Whittier, J. Hiratsuka, Y. Maeda, A. Hirai and M. Sugiura: "A physical map and clone bank of the rice chloroplast genome." Plant Mol. Biol. Report. 7. 284-291 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, H. and A. Hirai: "A Simple efficient method for identification of hybrids using nonradioactive rDNA as probe." Jpn. J. Breeding. 40. 339-348 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Arakawa, K., M. Katayama and T. Takabe: "Levels of betaine and betaine aldehyde dehydrogenase activity in the green leaves, and etiolated leaves and roots of barley." Plant Cell Physiol. 31. 797-803 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, H. and A. Hirai: "The gene for the alphasubunit of ATPase : a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 平井 篤志: "体細胞雑種植物の葉緑体ゲノムと植物体の再生" 細胞. 21. 220-223 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimada,H.: "A physical map and clone bank of the rice chloroplast genome." Plant Mol.Biol.Report.7. 284-291 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Honda,H.: "A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe." Jpn.J.Breeding. 40. 339-348 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Arakawa,K.: "Levels of betaine and betaine aldehyde dehydrogenase activity in the green leaves,and etiolated leaves and roots of barley." Plant Cell Physiol.31. 797-803 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 本田 秀夫: "非放射性DNAプロ-ブによる雑種の検定" 植物細胞工学. 3. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Honda,H.: "The gene for the αーsubunit of ATPase: a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells."

    • Related Report
      1990 Annual Research Report
  • [Publications] 平井篤志: "体細胞雑種植物の葉緑体ゲノムと植物体の再生" 細胞. 21. 220-223 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Honda,H.: "A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe." Jpn.J.Breeding.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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