| Project/Area Number |
01860010
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
土壌・肥料
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| Research Institution | Tohoku University |
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Principal Investigator |
OJIMA Kunihiko Tohoku Univ., Agriculture, Professor, 農学部, 教授 (70005669)
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| Co-Investigator(Kenkyū-buntansha) |
ARIHARA Akihiro Plant Biotech. Lab. Hokuren Staff, 農業総合研究所・育種研究所, 研究主任
OKAMURA Masachika Plant Biotech. Lab. Kirin, Staff, 植物開発研究所, 研究主任
YOKOTA Satoshi Tohoku Univ., Aguriculture, Assistant Prof, 農学部, 助手 (60220555)
SUGAWARA Kazuo Tohoku Univ., Aguriculture, associate Prof, 農学部附属農場, 助教授 (20005672)
SAIGUSA Masahiko Tohoku Univ., Aguriculture, associate Prof, 農学部附属農場, 助教授 (10005655)
山谷 知行 東北大学, 農学部, 助教授 (30144778)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1989: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | acid siol / aluminum tolerant / barley / carrot / plant tissue culture / stress selection / variant / volcanic ash soil / コムギ / イネ / アルミニウム / ストレス選抜 / アルミニウム耐性 / アルファルファ / 火山灰土壌 / 石灰施用 / リン酸肥料 / リン酸施用 |
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| Research Abstract |
Excess of aluminum-ion concentration is one of the most harmful factors on plant growth in acid soils. This research purpose was to produce a plant variant tolerant to higher concentration of ionic aluminum by useing the stress selection method in plant tissue culture. The experimental results were as follows.
(1) The cultured cells of barley (Hordeum Vulgare L. K-16 line) were plated and grown on agar medium containing 1mM aluminun to select aluminum-tolerant cell line. The selected cell line grew well better than nonselected cell line in the presence of 0.7mM aluminum. The barley plants were regenerated from nonselected- and selected cultures respectively. The seeds from the selected cell line grew better than those from nonselected cell cultures in a volcanic ash soil.
(2) Aluminum-tolerant variants from cell cultures of carrot (Daucus carota L. cv. Ms Yonsun) were selected by exposing them to excess ionic aluminum. The medium contained AlCl_3 and 0.1mM phosphate at pH 4.0. Under ionic aluminum stress (0.5-1.0mM), 282 calli were selected. Twenty-eight fertile plans were regenerated from 7 selected calli. Based on the root elongation tests of the seedlings, the seeds displayed aluminum-tolerance. In the case of the cell line H7-08, the selection occurred at a frequency of Ca. X 10^<-7> when the materials were plated on ager medium containing 0.5mM aluminum.
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