• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

System for fluorometry of carboxylic acids under mild condition

Research Project

Project/Area Number 01860014
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

OHRUI Hiroshi  Tohoku Univ., Agric., Assistant Prof, 農学部, 助教授 (20100050)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroaki  Tosoh, Chief Researcher, 主任研究員
AKASAKA Kazuaki  Tohoku Univ., Agric., Associate Prof., 農学部, 助手 (10201881)
NISHIDA Yoshihiro  Tohoku Univ., Agric., Associate Prof., 農学部, 助手 (80183896)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordshydroxy fatty acid / hydroperoxy fatty acid / 9-bromomethylacridine / column switching / 9-ブロモメチルアクリジン / 蛍光分析 / 脂肪酸 / 高速液体クロマトグラフィ- / 9ーブロムメチルアクリジン / 脂肪酸の定量 / プロスタグランジン / オカダ酸 / HPLCの新分離条件 / 9-acridinylmethyl ester / fatty acid / structural analysis / mass spectrometry
Research Abstract

9-Acridinylmethyl esters of fatty acids gave molecular ions with relatively strong intensity and simple fragmentation patterns. In the spectra of unsaturated fatty acid esters, the 26 mass unit separations were observed at the position of double bonds in the molecule. Ions (M-30)^+ and (M-31)^+ suggested the existence of a hydroxymethyl group and the 29 and 30 mass unit separations suggested the positions of hydroxy groups located in the molecule. These fragmentations were useful for the structural analysis of fatty acids.
Hydroxy and hydroperoxy fatty acids were labeled with 9-bromomethylacridine at room temperature. They were separated from the degradation produce and less polar fatty acid derivatives on an octyl silicagel column, and were loaded into an octadecyl silicagel column by the on-line column switching. By the method, picomolar levels of the derivatives were determined with good reproducibility. The detection limit of 16-hydroxyhexadecanoic acid as a representative was 0.9 pmol (S/N=3) and the relative standard deviation of its peak areas was 2.5% (18.5 pmpl,n=7). The method was applied to the determination of the hydroxy fatty acids derived from phosphatidylcholine (PC) hydroperoxides spiked in human plasma. During the incubation at 37゚C for 4h, 2.5-3% of PC hydroperoxides decreased gave the corresponding hydroxy fatty acids.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Kazuaki Akasaka: "Structural Analysis of 9-Acridinylmethyl Derivatives of Fatty Acids by Mass Spectrometry" Analytical sciences. 6. 511-514 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuaki Akasaka: "Determination of Hydroxy and Hydroperoxy Fatty Acids by a High Performance Liquid Chromatography with a column Switching System" Biosci.Biotech.Biochem.submited for publication.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuaki Akasaka: ""Structural Analysis of 9-Acridinylmethyl Derivatives of Fatty Acids by Mass Spectrometry"" Analytical Sciences. Vol. 6. 511-514 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuaki Akasaka: "Determination of Hydroxy and Hydroperoxy Fatty Acids by a High Performance Liquid Chromatography with a column Switching System"" Biosci. Biotech. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuaki AKASAKA: "Structural Analysis of aーAcridinylmethyl Derivatives of Fatty Acids by Mass Spectrometry" ANALYTICAL SCIENCES.6. 511-514 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大類洋: "新蛍光試薬の開発" 化学と生物. 27. 252-259 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuaki Akasaka: "Structural Analysis of 9-Acridinylmethyl Derivatives by Mass Spectrometry" Analytical Sciences.

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi