• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the development of ribosomal vaccine against pseudotuberculosis of yellowtail

Research Project

Project/Area Number 01860024
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionKochi University

Principal Investigator

KUSUDA Riichi  Kochi Univ., Faculty of Agriculture, Professor, 農学部, 教授 (90036715)

Co-Investigator(Kenkyū-buntansha) MURAOKA Aiichiro  Earth Chemical Co., Ltd., Biomedical Research Laboratories, Researcher, 研究第三部, 研究員
MIHARA Shigeru  Earth Chemical Co., Ltd., Biomedical Research Laboratories, Director, 研究開発部, 研究第三部長
NISHIMURA Akira  Earth Chemical Co., Ltd., Head office of Biomedical Research Laboratories, Manag, 研究開発本部, 専務取締役
KAWAI Kenji  Kochi Univ., Faculty of Agriculture, Assistant Professor, 農学部, 助教授 (60127925)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsYellowtail / Pseudotuberculosis / Ribosomal vaccine / Cellular resistance / Serum / Macrophage / Lymphocyte / Bactericidal activity / 有効性 / 貧食細胞 / 遊走能 / 貧食能 / 細胞内殺菌能 / Pasteurella piscicida / 抗原性 / 浸漬法ワクチン
Research Abstract

[Introduction] Ribosomal vaccine (RBP) of Pasteurella piscicida shows higher protection and longer durability by injection compared to that of formaling - killed cells (FKC). Present study investigated the mechanisms of protective immune responses in yellowtail induced by RBP.
[Materials and Methods] Fishes (mean body weight 5.4 g) were immunized with 160 ug of RNA of RBP prepared from P. piscicida by intraperitoneal injection at one week interval. Two control groups of fish were intraperitonealy injected with 1 mg of FKC and PBS, respectively. Fishes were taken out at 1, 2, 4 and 6 weeks after the second immunization and determined serological and cellular resistant indicators : bactericidal activity of the serum, accumulation reaction of peritoneal exdate cells (PEC), phagocytic activity and intracellular killing activity of macrophages (Mphi), bactericidal activity of peripheral blood lymphocytes (PBL).
[Results] The bactericidal activity of the serum was increased in the RBP group 4 … More weeks after the immunization, whereas in the FKC group, the increase is only slight after 2 and 4 weeks. The accumulation reaction of PEC changed only in the FKC group, where it increased after 2 weeks and decreased after 4 and 6 weeks. Phagocytic activity of Mphi changed only in the RBP group, where it increased after 4 weeks and decreased after 6 weeks. Intracellular killing activity of Mphi changed only in the FKC group, where it increased after 2 weeks and decreased after 4 and 6 weeks. Bactericidal activity of PBL increased after 2 weeks in the RBP group, but not in the FKC group. It can be concluded that RBP induces bactericidal activity of PBL after 2 weeks, and bactericidal activity of the serum along with phagocytic activity of Mphi after 4 weeks which all enhance total protective level of the fish. Whereas FKC induces accumulation reaction of PEC and intracellular killing activity of Mphi after 2 weeks. The difference of the protecive level induced by RBP and FKC is possibly attributed mainly to the bactericidal activity of PBL. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Riichi Kusuda: "Durability of induced resistance of yellowtail against pseudotuberculosis by the use of Pasteurella piscicida ribosomal and formalin-killed vaccines." Fish Pathology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya and Riichi Kusuda: "Antigenic properties of Pasteurella piscicida ribosomal vaccine" Bull.Japan.Soc.Fish.Sci.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya and Riishi Kusuda: "Comparison of the location of Pasteurella piscicida ribosomal vaccine in tissres of yellowtail after intraperitoneal and immersion immunization" Fish Pathology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya and Riichi Kusuda: "Comparison of the protective mechanism of yellowtail against pseudotuberculosis by the use of Pasteurella piscicida ribosomal and formalin-killed vaccines" Infect.Immun.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Riichi Kusuda: "The efficacy of ribosomal vaccine from Pasteurella piscicida against pseudotuberculosis in cultured yellowtail" Fish Pathol.23. 191-196 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya: "Effect of immersion vaccination of cultured yellowtail with a ribosomal vaccine prepared from Pasteurella piscicida" Bull. Japan. Soc. Sci. Fish.55. 1773-1776 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya: "Comparison of the durability of induced resistance of yellowtail against pseudotuberculosis by the use of Pasteurella piscicida ribosomal and formalin - killed vaccines" Fish Pathol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya: "Antigenic properties of Pasteurella piscicida ribosomal vaccine" Bull. Japan. Soc. Sci. Fish.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya: "Comparison of the location of Pasteurella piscicida ribosomal vaccine in tissues of yellowtail after intraperitoneal and immersion vaccination" Fish Pathol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Ninomiya: "Comparison of the protective mechanism of yellowtail against pseudotuberculosis by the use of Pasteurella piscicida ribosomal and formalin - killed vaccines" Infect. Immun.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 楠田 理一・二宮 学・浜口 昌已・村岡 愛一郎: "ブリ類結節症に対するPasteurella piscicidaリボゾ-ムワクチンの有効性" 魚病研究. 23. 191-196 (1988)

    • Related Report
      1991 Annual Research Report
  • [Publications] 二宮 学・村岡 愛一郎・楠田 理一: "Pasteurella piscicidaリボゾ-ムワクチンによるブリの浸漬免疫" 日本水産学会誌. 55. 1773-1776 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Riichi Kusuda: "Durability of induced resistance of yellowtail against pseudotuberculosis by the use of Pasteurella piscicida ribosomal and formalin-killed vaccines." Fish Pathology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Manabu Ninomiya and Riichi Kusuda: "Antigenic properties of Pasteurella piscicida ribosomal vaccine" Bull.Japan.Soc.Fish.Sci.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Manabu Ninomiya and Riichi Kusuda: "Comparison of the location of Pasteurella piscicida ribosomal vaccine in tissues of yellowtail after intraperitoneal and immersion immunization" Fish Pathology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Manabu Ninomiya and Riichi Kusuda: "Comparison of the protective mechanism of yellowtail against pseudotuberculosis by the use of Pasteurella piscicida ribosomal and formalin-killed vaccines" Infect.Immun.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 楠田 理一: "<Pasteurella>___ー <piscicida>___ーリボゾ-ムワクチンによるブリの免疫効果の経時的変化" 日本水産学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] 楠田 理一: "<Pasteurella>___ー <piscicida>___ーリボゾ-ムワクチンの抗原性" 魚病研究.

    • Related Report
      1990 Annual Research Report
  • [Publications] 二宮学,村岡愛一郎,楠田理一: "Pasteurella piscicidaのリボゾ-ムワクチンによるブリの浸漬免疫" 日本水産学会誌. 55. 1773-1776 (1989)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2019-02-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi