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Cell-dynamics Analyzing Apparatus with Multispectral Fluorescence Images Obtained by a Cooled-CCD Camera.

Research Project

Project/Area Number 01870007
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionFaculty of Medicine, University of Tokyo

Principal Investigator

TAKAHASHI Kunitaro  Faculty of Medicine, Department of Neurobiology, Institute of Brain Res., University of Tokyo, Professor, 医学部(医), 教授 (10010034)

Co-Investigator(Kenkyū-buntansha) AKATSUKA Takao  Faculty of Engineering, Department of Information Engineering, Yamagata Univ., P, 工学部, 教授 (80091875)
OKAMOTO Harumasa  Faculty of Medicine, Department of Neurobiology, Institute of Brain Res., Univer, 医学部(医), 助教授 (40134283)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 1990: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsMultispectral image / Interference-filter exchanger / Cooled-CCD camera / Material-distribution image / 胚細胞分化 / 細胞間相互作用 / 蛍光画像 / フィルタ切換装置
Research Abstract

This project aimed to design and make the multispectral image acquiring system consisting of an epifluorescence microscope, a pulse-motor driven interference-filter exchanger, a cooled-CCD camera, and a 32 bit personal computer. The filter exchanger apparatus was designed to insert one out of four interference filters alternatively into the relatively long parallel optical path designed to be placed between the object and eyepiece. The four filters were selected from a set of 11 bandpass filters with half transmittance width 20 nanometer covering the wave length range from 400 to 600 nanometer. The exchange was driven by a computer-controlled pulse motor for which commands were delivered through the interface of the personal computer. The fluorescence images were acquired through the noise-less, ultra-widely dynamically ranged and ultra-highly sensitive cooled-CCD camera. The image acquisition was performed by the commands from the personal computer through the GPIB interface. The embryonic preparation injected with multiple fluorescent dyes or fixed and stained with fluorescent probes were used for obtaining multiple spectral images with this system. The obtained images were analyzed by the programs to extract the material images from the multispectral images, such as proposed by Kawata et al. The programs performed the calculation of fluorescence intensity matrix. The eigen values of the matrix represented the ratios of principal components and the values helped to determine the number of the component chemicals which were responsible for the multispectral images. Then, knowing the spectra of the chemicals, it was possible to reconstruct the material images from multispectral images.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 高橋 國太郎: "実験系の初期発生と興奮膜" 実験医学. 7. 879-885 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado,H.: "Differentiation of membrane excitability in isolated cleavageーarrested blastomeres from early ascidian embryos." Journal of Physiology(London). 427. 583-602 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado,H.: "Induced neuralーtype differentiation in the cleavageーarrested blastomere isolated from early ascidian embryos." Journal of Physiology(London). 427. 603-623 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mitani,S.: "Embryonic development of Xenopus studies in a cell culture system with tissueーspecific monoclonal antibodies." Development. 105. 53-59 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mitani,S.: "Inductive differentiation of two neural lineages reconstituted in a microculture system from Xenopus early gastrula cells." Development. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado, H.: "Differentiation of membrane excitability in isolated cleavage-arrested blastomeres from early ascidian embryos." Journal of Physiology (London). 427. 583-602 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado, H.: "Induced neural-type differentiation in the cleavage-arrested blastomere isolated from early ascidian embryos." Journal of Physiology (London). 427. 603-623 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mitani, S.: "Embryonic developmental Xenopus studies in a cell culture system with tissue-specific monoclonal antibodies." Development. 105. 53-59 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mitani, S.: "Inductive differentiation of two neural lineages reconstituted in a microculture system from Xenopus early gastrula cells." Development. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado, H.: "Neural induction by the proteotytic enzyme 'pronase' in cleavage-arrested ascidian blastomere." Jpn. J. Physiol.39. S60 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado, H.: "Regional specificity of neural differentiation in ascidian ectodermal blastomeres and a possible mechanism of neural induction." Jpn. J. Physiol.40, Suppl.S106 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishizawa, K.: "Studies on inductive differentiation in Xenopus culture." Jpn. J. Physiol.40, Suppl.S14 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okado,H.: "Differentiation of membrane excitability in isolated cleavageーarrested blastomeres from early ascidian embryos." J.Physiol.Lond.427. 583-602 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Okado,H.: "Induced neuralーtype differentiation in the cleavageーarrested blastomere isolated from early ascidian embryos." J.Physiol.Lond.427. 603-623 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Okado,H.: "Regional specificity of neural differentiation in ascidian ectodermal blastomeres and a possible mechanism of neural induction." Jpn.J.Physiol.40Suppl.S106 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高橋國太郎: "実験系の初期発生と興奮膜" 実験医学. 7. 879-885 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okado,H.: "Neural induction by the proteotytic enzyme'pronase'in cleavage-arrested ascidian blastomere." Jpn.J.Physiol.39. S60 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okamura,Y.: "Suppression of transcription of inward rectifier K channel by neural induction in ascidian embryos." Neuroscience Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okado,H.: "Differentiation of membrane excitability in isolated cleavage-arrested blastomeres from early ascidian embryos." Journal of Physiology(London). (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okado,H.: "Induced neural-type differentiation in the cleavage-arrested blastomere isolated from early ascidian embryos." Journal of Physiology(London). (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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