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Development of a System for Analyzing Gene Expression in Small Specimen of Human Liver Tissue

Research Project

Project/Area Number 01870036
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

FUSAMOTO Hideyuki  Osaka University Medical School Lecturer, 医学部, 講師 (90124776)

Co-Investigator(Kenkyū-buntansha) KATAYAMA Kazuhiro  Osaka University Hospital Staff, 医学部附属病院, 医員
SASAKI Yutaka  Osaka University Medical School Assistant, 医学部, 助手
KASAHARA Akinori  Osaka University Medical School Assistant, 医学部, 助手 (70214286)
HAYASHI Norio  Osaka University Medical School Lecturer, 医学部, 講師 (00144478)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1990: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1989: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsGene expression / Proto-oncogene / DNA / PKC
Research Abstract

In order to clarify gene expressions in small specimen of liver tissue, we examined the expression of proto-oncogene and protein Kinase C (PKC) during liver regeneration. In this study, an in situ hybridyzation technique was applied for analysis. The role of proto-oncogenes in cell transformation ha been firmly established by both genetic and biochemical studies, but little is known about the role of these proto-oncogenes in normal cellular processes. Further, PKC plays a crucial role in signal transduction for a variety of biologically active substances which trigger cellular functions and proliferation. Expression of the c-Ha-ras proto-oncogene is specifically enhanced during liver regeneration, in parallel with increased DNA replication. In normal rat liver, a few hepatocytes expressed the mRNAs and the corresponding proteins without any preferential localization. Zonal heterogeneity of c-Ha-ras gene expression first became evident at 12 hr after CC14 administration, a higher number of gene products being detected in the pericentral zone than in the periportal zone. This heterogeneity became maximal at 24 hr after CC14 administration. The protein kinase C alpha subspecies was activated in a heterogeneous fashion, a higher number of hepatocytes expressing the protein kinase C alpha subspecies being detected in the pericentral zone than in the periportal zone. This zonal heterogeneity became maximal at 24 hr after the treatment. The distribution of hepatocytes expressing the protein kinase C alpha subspecies was roughly coincident with that of hepatocytes exhibiting of DNA synthesis. These results suggest that c-Ha-ras and protein kinase C may play a crucial role in liver regeneration.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Sasaki Y,at al: "Cellulan analysis of CーHaーras gane expression in rst liver aften CCl_4 administrotion." Hapatology. 10. 494-500 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki Y,et sl: "Heteroge activation of Protem Kinase C during rat liver regeneration indued hy cmbon titrechlovide administratration" FEBS LETTERS. 254. 59-65 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ito T,et al: "Seguentisl protooncogene expression during regeneration in rats stomach" Gastroenterology. 98. 1525-1531 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 三田 英治,他.: "慢性肝疾患における Cーmyc および CーHaーras 発現に関する検討" 肝臓. 31. 118-122 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 春名 能通,他.: "ラット肝化学発癌過程におけるrーGTP 転写発現動態の解折" 肝臓. 30. 649-655 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 伊藤 敏文,他.: "急性胃病変の粘評再生・修後過程におけるprotoーoncogene の発現動態" Therapeutic Research. 11. 26-31 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki Y, Hayashi N, Morita Y, Ito T, Kasahara A, Fusamoto H, Sato N, Tohyama M, Kamada T.: "Cellular analysis of c-Ha-ras gene expression in rat liver after CCl_4 administration." Hepatology. 10. 494-500 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki Y, Hayashi N, Ito T, Fusamoto H, Sato N, Kamada T.: "Heterogenous activation of protein kinase C during rat liver regeneration induced by carbon tetrachloride administration." Febs Letters. 254. 59-65 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ito T, Hayashi N, Sasaki Y, Morita Y, Kawano S, Fusamoto H, Sato N, Tohyama M, Kamada T.: "Sequential protooncogene expression during regeneration in rats stomach." Gastroenterology. 98. 1525-1531 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki Y,et al.: "Cellular analysis of cーHaーras gone expression in rat liver after CCl_4 administration." Hepatdogy. 10. 494-500 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sasaki Y,et al.: "Heteroge activation of protein kinase C during rat liver regeneration induced by carbon tetrachloride administration" FEBS LETTERS. 254. 59-65 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ito T,et al.: "Sequential proto oncogene expression during regeneration in rats stomach" Gastroenterology. 98. 1525-1531 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 三田 英治,他: "慢性肝疾患におけるcーmycおよびcーHaーras発現に関する検討" 肝臓. 31. 118-122 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 春名 能通,他.: "ラット肝化学発癌過程におけるγーGTP転写発現動態の解析" 肝臓. 30. 649-655 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊藤 敏文,他: "急性胃病変の粘膜再生・修復過程におけるprotoーoncogeneの発現動態." Therapentic Research. 11. 26-31 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sasaki Y,et al.: "Cellular analysis of c-Ha-ras gene expreesion in rat liver after CCl_4 administration." Hepatology. 10. 494-500 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 春名能通,他: "ラット肝化学発癌過程におけるγ-GTP転写発現動態の解析" 肝臓. 30. 649-655 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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