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Establishment of an animal model for Malignant hyperthermia

Research Project

Project/Area Number 01870067
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionHiroshima University

Principal Investigator

YUGE Osafumi (1991)  Hiroshima University School of medicine Professor, 医学部, 教授 (40034128)

盛生 倫夫 (1989-1990)  広島大学, 医学部, 教授 (80033950)

Co-Investigator(Kenkyū-buntansha) EBI Shigenao  Central Institution of Shimizukoushiryo, Chief, 中央研究所, 研究所長
MTSUBARA Toshimitu  Azabu Univ., School of Veterinary Medicine, Associate Professor, 獣医学部, 助教授 (50063954)
FUJII Kohyu  Hiroshima University, School of Medicine, Assistant Professor, 医学部, 講師 (60034021)
KIKUCHI Hirosato  Toho University, School of Medicine, Professor, 医学部, 教授 (40034029)
MORIO Michio  Chugoku Rosai general Hospital, Director, 院長 (80033950)
海老 茂直  清水港飼料株式会社, 中央研究所, 所長
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥31,200,000 (Direct Cost: ¥31,200,000)
Fiscal Year 1991: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1990: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1989: ¥24,100,000 (Direct Cost: ¥24,100,000)
KeywordsMalignant hyperthermia / Pietrain / procine stress syndrome (PSS) / Ca-induced Ca release / Sarwplasmic Reticulum / P.S.S.豚 / ピエトレン種豚 / ハロセンテスト / カルシウムによるカルシウム放出速度 / PSSブタ / スキンド骨格筋線維
Research Abstract

Malignant hyperthermia (MH) is one of the most hazardous complications encountered in anesthesia. It is assumed to be a genetic and metabolic disorder of the muscle, whose syndrome is triggered by anesthetics, depolarizing neuromuscular blocking agents and their related agents. The pure-line Pietrain pig is well known all over the world as a species that frequently develops porcine stress syndrome (PSS). PSS may be an animal model of MH observed in man. The purpose of this study was to import and culture the pure-line Pretrain pig and determine whether it is an animal model of human NM or not.
The pure-line Pietrain pigs of Belgian were imported from the Netherlands. These pigs were bred and then classified whether positive for PSS (PSS+) or negative (PSS-) by the halothane inhalation test (halo-test). The rate of Ca-induced Ca release (CICR) of skeletal muscle was not significantly deferent between the two pigs, but their values were significantly higher than those of other strains of pigs (Landrace-Yorkshire). All the pigs were anesthetize with halothane or sevoflurane. MH syndrome was observed in Pietrain pigs which were either PSS+ or PSS-pigs following anesthesia with these inhalation anesthetics. PaCO2 was elevated at first and thereafter metabolic acidosis made progressed. Next, body temperature began to rise, followed by hyperkalemia, high serum CPK and GOT, and elevated of other enzyme values. At last, all the pigs died within 100 minutes during halothane inhalation without any therapy, but MH syndrome was not observed in the other strains pigs.
We have concluded from these results that Pietrain pigs can be used as an animal model of human MH.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 松井 一幸他: "哺乳類体温における各種揮発性麻酔薬のモルモット骨格筋小胞体のCaーinduced Careleaseの作用" 麻酔と蘚生25巻別冊. 25(別). 27-30 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤岡 泰博他: "腹合撤性筋弛也緩薬サクラ-ルコリン・クロライドCaーinduced Carelease" 麻酔と蘇生. 25(別). 31-35 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松井 一幸他: "過去2年間におけるスキンドファイバ-法による悪性高然の診断" 麻酔と蘇生. 25(別). 35-59 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高橋 雅子他: "Ca^<2+>ーinduced Careleaseにおける正常値の検討" 麻酔と蘇生. 26(別). 37-40 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 向田 圭子: "骨格筋小胞体からのカルシウムによるカルシウム放出速度におよぼすエタノ-ルの影響ーモルモット、ピエトレンブタ骨格筋での検討ー" 麻酔と蘇生. 26. 361-368 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 海老 成直: "悪性高熱症モデルブタは畜産界ではどのように扱かわれているか" 医学のあゆみ. 158. 360 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松原 利光: "悪性高然症モデルブタの開発実験" 麻酔と蘇生. 27(別). 69-74 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久保田 稔: "悪性高熱症モデル豚(pietrain pig)の麻酔経験" 麻酔と蘇生. 27(別). 87-95 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Matsui et al.: "The effect of the Various Volatile Anesthetics on the Ca-induced Ca Release of the Sarwplasmiv Reticulum in the Skinned Skeletal Muscle from the ecuinea pig at the Mammalian Body Temperature" Hiroshima J. Anesth. 25(S). 27-30 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Fujioka, et al.: "Effects of Depalarizing Huscle Relaxant (Succinylcholine chloride) on Ca^<2+> Recruit ment in the skimed Muscle of the guinea pig" Hiroshima J. Aneths.25(S). 31-35 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Matsui, et al.: "The Diagnosis of Malignant Hyperthermia by the Skinned Fiber Test-the results of the last two years-." Hiroshima J. Aneths. 25(S). 55-59 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Takahashi et. al.: "Normal Value of Ca^<2+> induced Ca Release Rates in Human Sbeletal Muscle" Hiroshima J Aneths. 26(S). 37-40 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Mukaida: "Effects of Ethan of on the Ca^<2+> induced Ca^<2+> release Rate of Skeletal Muscles in guinea pigs and Rietrain pigs." Hiroshima J. Aneths. 26. 361-368 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Ebi: "An Inidel pig for malignant Hypertheiuia in the field of Zootechny." Igaku-no-Ayumi. 158. 360 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Matsubara: "Depelepment study Relative to Animal Hodcl of Hyperthernia Syndrone" Hiroshima J. Aneths. 27(S). 69-74 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Kubota. et al.: "Experience of Anesthesicu with Halathane in Malignant Hypertheria Suscaptible Sucine." Hiroshima J. Aneths. 27(S). 87-95 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 海老 成直: "悪性高熱症モデルブタは畜産界ではどのように及かわれているか" 医学のあゆみ. 158. 360- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松原 利光: "悪性高熱症モデルブタの開発実験" 麻酔と蘇生. 27(別). 69-74 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久保田 稔: "悪性高熱症モデル豚(pietlcin Pig)の麻酔経験" 麻酔と蘇生. 27(別). 87-95 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久保田 稔他: "悪性高熱症モデル豚(Piefrain pig)の麻酔経験" 第14回悪性高熱研究会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 松原 利光: "悪性高熱モデル動物の開発実験" 第14回悪性高熱研究会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤岡泰博他: "脱分極性筋弛緩薬サクシニルコリンクロライドとCaーusduced Ca release" 麻酔と蘇生. 25. (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2019-02-15  

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