| Project/Area Number |
01870079
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
OKUDA Reiichi Tohoku University, Dentistry, Professor, 歯学部, 教授 (80005024)
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| Co-Investigator(Kenkyū-buntansha) |
TAGAMI Atsushi Tohoku University, Dental Hospital, Assistant Professor, 歯学部附属病院, 助手 (00236374)
SHIMIZU Yoshinobu Tohoku University, Dentistry, Associate Professor, 歯学部, 助教授 (20005078)
KAGAYAMA Manabu Tohoku University, Dentistry, Professor, 歯学部, 教授 (60004610)
斎藤 素子 東北大学, 歯学部, 助手 (80215566)
斎藤 修 東北大学, 歯学部付属病院, 助手 (50178480)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1991: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1990: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1989: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | Artificial Dental Root / Pure Titanium / Periodontal Ligament Cells / Cell Culture / Suppression of Bone Resorption / Alkaline Phosphatase / 歯根膜形成 / 歯根膜細胞性状 / 立体培養 / 骨吸収活性抑制 / 骨形成活性高揚 / 表面形状 |
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| Research Abstract |
The purpose of this study is the establishment of artificial dental root, which is functionally close to the natural teeth. In the process of the developing technique to make periodontal ligament (PDL) on the surface of titanium artificial root, we investigated the character of PDL cells invitro or in vivo. The main results observed were as follows.
1. The primary cultivation of human PDL cells could be obtained at the rate of success 90% or more with our improved method. 2. Human PDL cultured cells could be adsorbed on the titanium surface. 3. Cell-Tak coating could accelerate the absorbance of human PDL cells onto titanium surface. 4. The growth velocity of PDL cells was the most accelerated in the medium supplemented with 10% human serum. 5. The polished and glass beads-sandblasted titanium surface were appropriate for the growth of PDL cells. 6. The factor produced from PDL cells could suppress the induction of osteoclast from the cultured peripheral blood lymphocytes and monocytes (PBL). 7. Gingival cells and osteoblast originated from alveolar bone didn't produce this suppressing factor. 8. Although number of osteoclast induced from the PBL could be increased by the stimulation of some bacteria from periodontal lesion, the addition of the culture fluid of PDL cells significantly decreased the induction of osteoclast from PBL stimulated with bacterias. 9. ALPase activity of PDL cells was not suppressed by co-culture with PBL. 10. As compared with gingival cells, ALPase activity and collagen synthesis of PDL cells was remarkably higher. 11.10th Passage of cells did not show in any defective effect on differentiating function of PDL cells.
Concerning casting system of titanium, development on the investment and the technical improvement on design of sprue and vent were also attained.
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