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Apatite-collagen complex as a possible bone substitute

Research Project

Project/Area Number 01870084
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 補綴理工系歯学
Research InstitutionAsahi University, School of Dentistry

Principal Investigator

DOI Yutaka  Asahi Univ., School of Dent., Associate Professor, 歯学部, 助教授 (40116067)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Noriko  Asahi Univ., School of Dent., Researcher, 歯学部, 助手 (00210865)
ADACHI Masanori  Asahi Univ., School of Dent., Researcher, 歯学部, 助手 (60076057)
WAKAMATSU Nobukazu  Asahi Univ., School of Dent., Researcher, 歯学部, 助手 (00158594)
GOTO Takayasu  Asahi Univ., School of Dent., Instructor, 歯学部, 講師 (30121320)
MORIWAKI Yutaka  Asahi Univ., School of Dent., Professor, 歯学部, 教授 (90028738)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥8,900,000 (Direct Cost: ¥8,900,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1989: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsBone Replacement / Apatite / Collagen / Bone Substitute / Biocompatibiltiy / Alkaline Phosphatase / Porous Apatite / Phosvitin / 架橋体 / アパタイトセメント / 多孔体アパタイト
Research Abstract

A new apatite-collagen complex was prepared in calcium beta-glycerophosphate solutions at pH 8.50. In one preparation, reconstituted type I collagen was cross-linked with phosvitin in the presence of alkaline phosphatase by the use of cross-linking agent of dimethyl subrimidate. After two weeks of immersion in daily-renewed solution of calcium beta-glycerophosphate, apatite deposited on the collagen fibrils. The apatitte-collagen complex thus prepared contained apatite approximately two times the modified collagen in weight. When viewed under a scanning electron microscope, needle-like crystals precipitated densely on the collagen fibrils. In some portion of the complex, however, dotlike precipitate was observed as well. X-ray diffraction and IR analyses of the complex suggested that apatite precipitated on the collagen fibrils was very similar to bone mineral in two aspects, crystallinity and carbonate content. When implanted for one month as a bone substitute in dog jaw after teeth w … More ere extracted, the complex was found resorbed without no substantial foreign body response. The site where the complex was implanted was filled with newly formed bone. This finding may suggest that the apatite-collagen, -complex prepared here is biodegrable and osteogeneiic as well.
In the second preparation, atelocollagen sheets (MEIPAK) with apatite deposited on were laminated in apatite cement consisting of an equisolar mixture of Te-CP and DCPD under isostatic pressure of 600 MPa, which was later immersed in distilled water at 37゚C for 7 days to completely transform the sement portion into apatite. The sheet-collagen laminated apatite blocks thus obtained were subjected to four points bending test with a loading direction perpendicular to the sheets inside the block. The load was plotted as a function of longitudinal displacement of the block specimen. Comparison of data obtained from the block specimens with and without the collagen sheets showed that presence of the collagen sheet failed to increase the bending strength but increased the work done on the specimen. Non-treated collagen sheet was also tried to laminate in apatite. However, it was practically impossible to prepare compacted blocks. This finding suggests that predeposition of apatite crystals on collagen sheet is a key step to successfully combine collagen and apatite, indicating that apatite deposited on collagen sheet acts as seed crystal for the sement setting process. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Yutaka Doi et al.: "Ostecnectin Inhibiting De Novo Formation of Apatite in the Presence of Collagen" Calcif.Tissue Int.44. 200-208 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 堀口 敬司他: "アパタイトーコラ-ゲン複合体ー複合体製作の可能性ー" 歯材器誌. 9. 863-870 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 堀口 敬司他: "アパタイトーコラ-ゲン複合体 II.石灰化溶液の検討" 歯材器誌. 10. 814-819 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 若松 宣一他: "金属表面にコ-ティングしたハイドロキシアパタイト層の焼結収縮" 歯材器誌. 11. 65-76 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Effects of non-collagenous proteins on the formation of apatite in calcium β-glycerophosphate solutions." Archs oral Biol.37. 15-21 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Apatite-collagen complex as bone substitute." J.Dent.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Self-setting apatite cement.New materials as canal filling and bone substitute." Materials Research Society, 6 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Effects of enamel,bone and dentin matrix proteins on precipitation of calcium phosphate in agarose gel." Florence publishers, 9 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Self-setting apatite cement. New materials as canal filling and bone substitute" Proceedings of the MRS Internationa Meeting on Advanced Materials, 1989 Mater. Res. Soci.1. 415-420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Effects of enamel, bone and dentin matrix proteins on precipitation of calcium phosphate in agarose gel." Proceedings of the Fifth International Symposium on the Composition, Properties, and Fundamental Structure of Tooth Enamel, and related Tissues, 1989 Florence Pub.511-518

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Osteonectin Inhibiting De Novo Formation of Apatite in the Presence of Collagen." Calcif Tissue Int. 44. 200-208 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yasumasa Takezawa et al.: "Self-setting apatite cement. VI Possibility as bone substitute." Jap J Oral Biol. 31. 240-247 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shunichi Shibata et al.: "Self-setting Apatite Cement. VIII. Dissolution and Remineralization Behavior of ^<45>Ca-apatite Cement." J J Dent Mater. 8. 812-817 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Horiguchi et al.: "Apatite-collagen complex. -Preparatioon of a new apatite-collagen complex-" J J Dent Mater. 9. 863-870 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Horiguchi et al.: "Apatite-collagen complex. II. Optimum conditions of mineralizing solution." J J Dent Mater. 10. 814-819 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobukazu Wakamatsu et al.: "Sintering Shrinkage of Hydroxyapatite Layer Coated on the Metal Substrate." J J Dent Mater. 11. 65-76 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Effects of non-collagenous proteins on the formation of apatite in calciuum beta-glycerophosphate solutions." Archs oral Biol. 37. 15-21 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Doi et al.: "Osteonectin Inhibiting De Novo Formation of Apatite in the Presence of Collagen" Calcif.Tissue Int.44. 200-208 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 堀口 敬司他: "アパタイト-コラ-ゲン複合体-複合体製作の可能性-" 歯材器誌. 9. 863-870 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 堀口 敬司他: "アパタイト-コラ-ゲン複合体 II.石灰化溶液の検討" 歯材器誌. 10. 814-819 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 若松 宣一他: "金属表面にコ-ティングしたハイドロキシアパタイト層の焼結収縮" 歯材器誌. 11. 65-76 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Doi et al.: "Effects of non-collagenous proteins on the formation of apatite in calcium β-glycerophosphate solutions." Archs oral Biol.37. 15-21 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Doi et al.: "Apatite-collagen complex as bone substitute." J.Dent.Res.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Doi et al.: "Self-setting apatite Cement.New materials as canal filling and bone substitute" Materials Research Society, 6 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Doi et al.: "Effects of enamel,bone and dentin matrix proteins on precipitation of calcium phosphate in agorose gel." Florence Publishers, 9 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Doi,et al.: "Osteonetin Inhikiting De Novo Formation of Apatite in the Presence of Collagen" Calcif.Tissue Int.44. 200-208 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹沢 保政他: "自己硬化型アパタイトセメントVI.骨補填材としての可能性" 歯科基礎医学会誌. 31. 240-247 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 柴田 俊一他: "自己硬化型アパタイトセメントVIII. ^<45>Caーアパタイトセメントの溶解性と再石灰化" 歯材器誌. 8. 812-817 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 若松 宣一他: "金属にコ-ティングしたハイドロキシアパタイトセラミックスの空気中及び蒸留水中での曲げ強さのワイブル統計処理" 歯材器誌. 9. 165-177 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 若松 宣一他: "蒸留水(37℃)中でのハイドロキシアパタイトコ-ディング層の疲労パラメ-タ-の推定とSPT線図を用いた寿命の予測" 歯材器誌. 9. 178-188 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 堀口 敬司他: "アパタイトーコラ-ゲンの複合化I.エピタキシャル的結合" 歯材器誌. 9. 863-870 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yutaka Doi,et al.: "Selfーsetting apatite cement New materials as canal filling and bone substutute." Materials Research Society, 6 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yutaka Doi,et al.: "Effects of enamel,bone and dentin matrix proteins on precipitation of calcium phosphate in agarose gel." 9 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yutaka Doi et al.: "Osteonectin Inhibiting De Novo Formation of Apatie in the Presence of Collagen" Calcif Tissue Int. 44. 200-208 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 竹沢保政他: "自己硬化型アパタイトセメントVI.骨補填材としての可能性" 歯科基礎医誌. 31. 240-247 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 柴田俊一他: "自己硬化型アパタイトセメント VIII.^<45>Ca-アパタイトセメントの溶解と再石灰化" 歯材器誌. 8. 812-817 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 若松宣一他: "金属にコ-ティングしたハイドロキシアパタイトセラミックスの空気中及び蒸留水中での曲げ強さのワイブル統計処理" 歯材器誌. 9. 165-177 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 若松宣一他: "蒸留水(37℃)中でのハイドロキシアパタイトコ-ティング層の疲労パラメ-タの推定とSPT線図を用いた寿命の予測" 歯材器誌. 9. 178-188 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 堀口敬司他: "アパタイトとコラ-ゲンの複合化 I.エピタキシャル的結合" 歯材器誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] Yutaka Doi et al.: "Self-setting apatite cement.New materials as canal filling and bone substitute." Materials Research Society, 6 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yutaka Doi et al: "Effects of enamel,bone and dentin matrix proteins on precipitation of calcium phosphate in agarose gel." Florence Publishers, 9 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2019-02-15  

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