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The Development of Bone Substitute Added with Bone Morphogenetic Protein and Clinical Application

Research Project

Project/Area Number 01870086
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionYamanashi Medical College

Principal Investigator

YAMAZAKI Yasuharu  Yamanashi Medical College, Faculty of Medicine, Lecturer, 医学部, 講師 (00210401)

Co-Investigator(Kenkyū-buntansha) OHNISHI Masatoshi  Yamanashi Medical College, Faculty of Medicine, Professor, 医学部, 教授 (50014139)
OIDA Shinichiro  Tokyo Medical and Dental University Faculty of Dentistry, Research Associate, 歯学部, 助手 (10114745)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsBone morphogenetic protein / Bone induction / Bone substitute / Plaster of paris / Delivery system / Bone conduction / TGF-β / 骨補填材
Research Abstract

[Bone Morphogenetic Protein (BMP)]
Amino acid sequences of six bone morphogenetic proteins (BMPs) were reported by Wozney, j. et al. The amino acid sequences for our final highly purified protein from bovine bone show considerable homology to BMP-3.
[Bone substitute added with BMP]
In TCP (Tricalcium phosphate) or HA (Hydroxyapatite) mixed with BMP implanted into the mouse femoral muscle, it was confirmed that BMP induced bone formation around and on the surface of those particles. There were no difference between the yield of new bone of BMP/HA (Hydroxyapatite) or BMP/TCP (Tricalcium phosphate) composite. However, We found the yield of new bone of it was less than that of BMP alone. This would appeared to indicate that BMP was spread within a short period in vivo, and tissue differentiation was disturbed by HA or TCP. Therefore, an effective BMP delivery system from synthetic vehicle for concentrating BMP was required. Then we investigated the morphologic sequence of a response to a BMP/ … More PLP (Plaster of Paris) composite implanted into the mouse femoral muscle. Minimum crude BMP alone dose for bone induction was needed 3mg, on the other hand, in BMP/PLP (1 : 5) composite, that was reduced up to one mg. In more purified BMP (the activity was four times greater than that of crude-BMP), BMP dose was, furthermore, decreased up to 0.25mg in BMP/PLP (1 : 10) composite. Also the BMP/PLP composite implanted into the bone area of rat and monkey induced active bone formation. These results suggest that PLP composite system is one of the suitable BMP delivery systems currently available.
[Clinical application]
For the observation of clinical usefulness of BMP, autodecalcified dentin, it was already reported that BMP was included in the dentin, was prepared. That powder was implanted into bone defects after resection of oral and maxillofacial diseases. In results, autodecalcified dentin did not cause a foreign body reaction and inflammation, but we did not, contrary to expectation, confirmed bone formation induced by that dentin in roentgenograms. The some cause of it is to be sought that in partly clinical detection methods of induced bone by BMP, almost the deficiency of BMP in autodecalcified dentin and target cell in host tissue. These important problems must be solved in future. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Yamazaki,Y.: "Experimental study on an implant of a composite of bone morphogenetic protein and calcium biomaterials." Society for Biomaterials,. 12. 218 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamazaki,Y.: "Ectopic induction of cartilage and bone by bovine bone morphogenetic protein using a biodegradable polymer capsule." Society for Biomaterials,. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oida,S.: "Bone morphogenetic protein (BMP) and transforming growth factorーβ(TGFーβ)in human bone matrix." Clinical Orthopaedics and Related Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oida,S.: "Osteogenic Potential of the cartilage induced in vitro by soluble factors present in bone."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 山崎 安晴: "骨形成因子(骨誘導物質)" 臨床検査. 3月号. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 大西 正俊(分担): "口腔癌 ー診断と治療ー (顎骨の再建)" デンタル ダイヤモンド社, 9 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuharu Yamazaki: "Experimental study on an implant of a composite of bone morphogenetic protein and calcium biomaterials." Society for Biomaterials. 12. 218 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuharu Yamazaki: "Ectopic induction of cartilage and bone by bovine bone morphogenetic protein using a biodegradable polymer capsule." Society for Biomaterials. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shinichiro Oida: "Bone morphogenetic protein (BMP) and transforming growth factor-beta (TGF-beta) in human bone matrix." Clin. Orthop.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shinichiro Oida: "Osteogenic Potential of the cartilage induced in vitro by soluble factors present in bone."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamazaki,Y.: "Experimental study on an implant of a composite of bone morphogenetic protein and calcium biomaterials." Society for Biomaterials.12. 218 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamazaki,Y.: "Ectopic induction of cartilage and bone by bovine bone morphogenetic protein using a biodegradable polymer capsule." Society for Biomaterials.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Oida,S.: "Bone morphogenetic protein (BMP) and transforming growth factorーβ (TGFーβ) in human bone matrix." Clinical Orthopaedics and Related Research.

    • Related Report
      1990 Annual Research Report
  • [Publications] Oida,S.: "Osteogenic Potential of the cartilage induced in vitro by soluble factors present in bone."

    • Related Report
      1990 Annual Research Report
  • [Publications] 山崎 安晴: "骨形成因子(骨誘導物質)" 臨床検査(3月号). (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大西 正俊(分担): "口腔癌ー診断と治療ー(顎骨の再建)" デンタルダイヤモンド社, 9 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamazaki,Y.: "Experimental study on an implant of a composite of bone morphogenetic protein and calcium biomaterials" Transactions of the Society for Biomaterials. 12. 218 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamazaki,Y.: "Response of the mouse femoral muscle to an implant of a composite of bone morphogenetic protein and plaster of Paris" Clinical Orthopaedics and Related Research. 234. 240-249 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大西正俊(分担): "口腔癌ー診断と治療ー(顎骨の再建)" デンタルダイヤモンド社, 9 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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