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Development of Passive Immunization System Against Dental Caries by Hen Egg Yolk Antibodies of Streptococcus Mutans.

Research Project

Project/Area Number 01870089
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionOsaka University Faculty of Dentistry

Principal Investigator

OOSHIMA Takashi  Osaka Univ. Facul. Dent., Associate Prof., 歯学部, 助教授 (80116003)

Co-Investigator(Kenkyū-buntansha) OGAWA Tomohiko  Osaka Univ. Facul. Dent., Assistant., 歯学部, 助手 (80160761)
TAKADA Haruhiko  Osaka Univ. Facul. Dent., Assistant Prof., 歯学部, 助教授 (30135743)
IZUMITANI Akira  Osaka Univ. Facul. Dent., Assistant Prof., 歯学部附属病院, 講師 (70168277)
MORISAKI Ichijiro  Osaka Univ. Facul. Dent., Associate Prof., 歯学部附属病院, 助教授 (30116115)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1990: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1989: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordsStreptococcus mutans / Glucosyltransferase / Oral passive immunization / Dental caries / Egg yolk antibody / Stroptococcus mutans / 受動免疫
Research Abstract

The effect of polyclonal egg yolk antibodies (yIgG) raised against whole cells, cell-free (CF) glucosyltransferase (GTase), cell-associated (CA) GTase, surface protein antigen (PAc) of serotype c S. Mutans was examined in terms of in vitro inhibition of virulence-related factors of S. mutans and protection of S. mutans-infected rats against the development of dental caries. Hens (18 weeks old) were immunized with formalintreated whole cells, purified CF-GTase, CA-GTase or PAc together with Freund complete adjuvant. yIgG was purified by ammonium sulfate precipitation followed by DEAE-Sephacel column chromatography or fractional precipitation with ethanol. Purified yIgG was found to be a 220-kDa protein, which was dissociated into H and L chains upon addition of 2-mercaptoethanol. yIgG to whole cells gave a heavy aggregation of the organisms of S. mutans, while yIgG to CF-and CA-GTase specifically inhibited the enzymatic activity of these GTases, respectively. yIgG to CA-GTase and whole cells was found to suppress clearly the cell adherence of S. mutans to a glass surface. Specific-pathogen-free Sprague-Dawley rats that had been infected heavily and repeatedly with S. Mutans and fed diet #2000 induced severe dental caries, while rats fed diet #2000 containing>0.1% yIgG to CA-GTase resulted in a statistically significant reduction in dental plaque accumulation and caries development. Administration of yIgG to CF-GTase failed to protect against caries. These results clearly suggest that yIgG to S. mutants CA-GTase specifically inhibited a virulence factor of this organism, i. e. Insoluble glucan synthesizing CA-GTase, resulting in a significant drop in the development of dental caries.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 堀越 俊雄: "Streptococws mutansの菌体結合型クルコシルトランスフェラ-ゼに対するニワトリ卵黄抗体によるラット実験う蝕の抑制" 日本細菌学雑誌. 46. 152 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigeyuki Hamada: "Oral passive immunization against dental caries in rats by hen eqq yolk.antibodies specific for cellーassociated glucosyltransferase of Streptococcus mutans"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigeyuki Hamada: "Streptococcus mutans antigens and oral passive immunization against dental caries with hen egg yolk antibodies to the antigens.In Proceedings of 6th international congress of mucosal immunoloqy." Elsevier Science Publishers, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigeyuki Hamada: "Streptococcus mutans antigens and oral passive immunization against dental caries with hen egg yolk antibodies to the antigens." Proceedings of 6th International Congress of Mucosal Immunology. Elsevier Science Publishers.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshio Horikoshi: "Oral passive immunization against dental caries in rats by hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans" Jpn J Bacteria. 46. 152 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takashi Ooshima: "Effect of experimental hyposalivation on the induction of dental caries in rats infected with Streptococcus mutans." Caries Res. 24. 446-451 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 堀越 俊雄: "Streptococcus mutansの菌体結合型クルコンルトランスフェラ-ゼに対するニワトリ卵黄抗体によるラット実験う蝕の抑制" 日本細菌学雑誌. 46. 152 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigeyuki Hamada: "Oral passive immunization against dental caries in rats by hen egg yolk antibodies specific for cellーassociated glucosyltransferase of Streptococcus mutans"

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigeyuki Hamada: "Streptococcus mutans antigens and oral passive immunization against dental caries with hen yolk antibodies to the antigens.In “Proceedings of 6th International Congress of Mucosal Immunology"" Elsevier Science Publishers, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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