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Developments of Rapid and Convenient New Diagnostic Method for Chagas' Disease.

Research Project

Project/Area Number 01870115
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionNagasaki University

Principal Investigator

KITAGAWA Tsunehiro  Nagasaki University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (70039634)

Co-Investigator(Kenkyū-buntansha) KANBARA Hiroji  Nagasaki University, Institute of Tropical Medicines, Professor, 教授 (20029789)
TANIMORI Hideaki  Nagasaki University, Faculty of Pharmaceutical Sciences, assistant, 薬学部, 助手 (70159028)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1990: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1989: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsChagas' Disease / New Diagnostic Method / Trypanosoma Cruzi / Epimastigote / Trypomastigote / Amastigote / Immunoassay / New Method
Research Abstract

The Protozoan Trypanosoma cruzi, the etiologic agent of Chagas' disease, consist of a variety of different strains. There are 3 life-cycle forms of T. Cruzi. Epimastigotes multiply in the insect vector ; infectious trypomastigotes enter mammalian host cells and differentiate into amastigotes. Intracellular amastigotes multiply in host cells. The present study was aimed to develop enzyme immunoassay (EIA) methods for T. Cruzi of all three life-cycle forms. 1. EIA for T. Cruzi, trypomastigotes (a new diagnostic method for Chaga's disease) : Two EIA methods were developed : one is specific assay for Tulahuen strain and another one is general detection method for all strains of trypomastigotes. The later method is expected to be a useful diagnostic method for Chagas' disease, though its sensitivity should be improved. 2. The Eia for epimastigotes developed is a general assay for all strains of epimastigotes. The method could be a new diagnostic method for the detection of T. Cruzi infected insect vectors. 3. EIA specific to amastigotes of Tulahuen strain was developed. The method was successfully applied for the first detailed analysis of infection states of various organs of mice infected by T. Cruzi Tulahuen strain. In summary, we developed EIAs for all three life-cycle forms of T. Cruzi. All the methods were expected to be new useful diagnostic methods for various studies of Chagas' disease, some of which have already demonstrated.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] H.Yanimori,T.: "A new liquidーPhase enzyme immunoassay of rabbit IgM and its application for comparing specificity of IgM and IgG contained in the same antiserum." Journal of Immunological Methods. 129. 233-242 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] JーG.Hu,T.Yokoyama T.Kitagawa: "Studies on the optimal immunization schedule of experimental animals.IV.The optimal age and sex of mice,and the influence of schedule of booster injection." Chemical Pharmaceutical Bulletin. 38. 448-451 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] JーG.Hu,T.Yokoyama T.Kitagawa: "Studies on the optimal immunization shcdule of experimental animals.V.The effects of the route of injection,the content of Mycobacteria in Freund's adjucant and the emulsifying antigen." Chemical Pharmaceutical Bulletin. 38. 1961-1965 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] JーG.Hu,T.kitagawa: "Studies on the optimal immunization shcedule of experimental animals.VI.Antigen doseーrsponse of aluminium hydroxideーaided immunization and booster effect under Iow antigen dose." chemical Pharmaceutical Bulletin. 38. 2775-2779 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Tanimori,T.Maeda,A.Akahori,S.Sonoi,: "Development and application of a new method for assay of the content of hoelen extract in Chinese traditional medicines." Chemical Pharmaceutical Bulletin. 38. 3060-3068 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Fujiwara,N.Matsumoto,T.Kitagawa: "The use of Nー(aminobenzoyloxy)succinimide as a twoーlevel beterobifunctional agent for the preparation of ^1aptenproten conjugates.Daunomycin as a model hapten with an amino group." Journal of Immunological Methods. 134. 227-235 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Chard著:北川 常廣,藤原 邦雄,谷森 英明 訳: "ラジオイムノアッセイ:制化学実験法" 東京化学同人, (245)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kitagawa, Y. Sakamoto, K. Furumi, H. Ogura.: "Novel enzyme immunoassays for specific detection of Fusarium oxysporum f. sp. cucumerinum and for general detection of various Fusarium species." Phytopathology. 79. 162-165 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kitagawa, H. Tanimori, M. Shibata, K. Yoshida, J-G, Hu: "Analyses of specific and total antibody responses of rabbits to four kinds of immunogens." Chem. Pharm. Bull.37. 1013-1018 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kitagawa, H. Tanimori, Y. Kohno, A. Akahori, S. Sonoi, S. Kondo.: "A new method for the quantitative analysis of the aqueous extract of Pinellia tuber contained in several chinese traditional medicines." Chem. Pharm. Bull.37. 1134-1136 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J-G, Hu, H. Tanimori, M. Shibata, T. Yokoyama, T. Kitagawa.: "Comparative study of complete and incomplete Freund's adjuvants for immunization of a drug immunogen using enzyme immunoassay methods." Chem. Pharm. Bull.37. 1316-1320 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J-G, Hu, A. Ide, T. Yokoyama, T. Kitagawa.: "Studies on the optimal immunization shedule of experimental animals. The effect of antigen dose and adjuvant type." Chem. Pharm. Bull.37. 3042-3046 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kitagawa. Y. Sakamoto, H. Ogura.: "A novel enzyme immunoassay for conidia of Fusarium oxysporum f. sp. cucumerinum F504." Microbiol. Immunol.33. 775-785 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tanimori, and T. Kitagawa.: "A new liquid-phase enzyme immunoassay of rabbit IgM and its application for comparing specificity of IgM and IgG contained in the same antiserum." J. Immunol. Methods.129. 233-242 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J-G, Hu, T. Yokoyama, T. Kitagawa.: "Studies on the optimal immunization schedule of experimental animals. IV. The optimal age and sex of mice, and the influence of the schedule of booster injection." Chem. Pharm. Bull.38. 448-451 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J-G, Hu, T. Yokoyama, T. Kitagawa.: "Studies on the optimal immunization schedule of experimental animals. V. The effects of the route of injection, the content of Microbacteria in Freund's adjuvant and the emulsifying antigen." Chem. Pharm. Bull.38. 1961-1965 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J-G, Hu T. Kitagawa.: "Studies on the optimal immunization schedule of experimental animals. VI. Antigen dose-response of aluminum hydroxide-aided immunization and booster effect under low antigen dose." Chem. Pharm. Bull.38, (10). 2775-2779 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tanimori, T. Maeda, A. Akahori, S. Sonoi, S. Kondo, T. Kitagawa.: "Development and application of a new method for assay of the content of hoelen extract in Chinese traditional medicines." Chem. Pharm. Bull.38. 3064-3068 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Fujiwara, N. Matsumoto, T. Kitagawa, K. Inoue.: "The use of N-(aminobenzoyloxy) succinimide as a two-level hetero-bifunctional agent for the preparation of hapten protein conugates. Daunomycin as a model hapten with an amino group." J. Immunol. Methods.134. 227-235 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tanimori, A. Akahori, S. Sonoi, T. Kitagawa.: "A new enzyme immunoassay for a solid Chinese crude drug Pinellia tuber." Chem. Pharm. Bull.39, no 6. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Tanimori,T.Kitagawa: "A new liquidーphase enzyme immunoassay of rabbit IgM and its application for comparing specificity of IgM and IgG contained in the same antiserum." Journal of Immunological Methods. 129. 233-242 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] JーG.Hu,T.Yokoyama,T.Kitagawa: "Studies on the optimal immunization schedule of experimental animals.IV.The optimal age and sex of mice,and the influence of the schedule of booster injection." Chemical Pharmaceutical Bulletin. 38. 448-451 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] JーG.Hu,T.Yokoyama,T.Kitagawa: "Studies on the optimal immunization shcdule of experimental animals.V.The effects of the route of injection,the content of Mycobacteria in Freund's adjucant and the emulsifying antigen." Chemical Pharmaceutical Bulletin. 38. 1961-1965 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] JーG.Hu,T.Kitagawa: "Studies on the optimal immunization shcedule of experimental animals.VI.Antigen doseーresponse of aluminium hydroxideーaided immunization and booster effect under low antigen dose." Chemical Pharmaceutical Bulletin. 38. 2775-2779 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Tanimori,T.Maeda,A.Akahori,S.Sonoi,: "Development and application of a new method for assay of the content of hoelen extract in Chinese traditional medicines." Chemical Pharmaceutical Bulletin. 38. 3060-3068 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Fujiwara,N.Matsumoto,T.Kitagawa: "The use of Nー(aminobenzoyloxy)succinimide as a twoーlevel beterobifunctional agent for the preparation of haptenーproten conjugates.Daunomycin as a model hapten with an amino group." Journal of Immunological Methods. 134. 227-235 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T、Chard著:北川 常廣、藤原 邦雄、谷森 英明訳: "ラジオイムノアッセイ:生化学実験法" 東京化学同人, 245 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kitagawa,Y.Sakamoto,K.Furumi and H.Ogura: "Novel enzyme immunoassays for specific detection of Fusarium oxysporum f.sp.cucumerinum and for general detection of various Fusarium species." Phytopathology. 79. 132-135 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kitagawa,H.Tanimori,M.Shibata,Y.Yoshida,and J-G.Hu: "Analyses of specific and total antibody responses of rabbits to four kinds of inmmunogens." Chem.Pharm.Bull.37. 1013-1018 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kitagawa,H.Tanimori,M.Shibata,T.Yokoyama,and T.Kitagawa: "Comparative study of complete and incomplete Freund's adjuvant for inmmunization of a drug immunogen using enzyme immunoassay methods." Chem Pharm.Bull.37. 1316-1320 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kitagawa,H.Tanimori,K.Kohno,A.Akabori,and T.Kitagawa: "A new method for quantitative analysis of the aqueous extract of Penellia tuber contained in several chinese traditional medicines." Chem.Pharm.Bull.37. 1134-1136 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] J-G.Hu,A.Ide,T.yokoyama,and T.Kitagawa: "Studies on the optimal immunization schedule of experimental animals.The effect of antigen dose and adjuvant type." Chem.Pharm.Bull.37. 3042-3046 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kitagawa,Y.Sakamoto,and H.Ogura: "A novel enzyme immunoassay for conidia of Fusarium oxysporum f.sp.cucumerinum F504." Microbiol.Immunol.33. 775-785 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Tanimori,and T.Kitagawa: "A new liquid-phase enzyme immunoassay of rabbit IgM and its application for comparing specificity of IgM and IgG contained in the same antiserum." J.Immunol.Methods. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] J-H.Hu,T.Yokoyama,and: "Studies on the optimal immunization shcedule of experimental animals.IV.The optimal age and sex of mice,and the influence of the schedule of booster injection." Chem.Pharm.Bull.38. 448-451 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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