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Animal Experimental Model of Human Hepatocellular Carcinoma

Research Project

Project/Area Number 01880010
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

SHIGA Junji  University of Tokyo Pathology Associate Professor, 医学部(病)病理, 助教授 (10110694)

Co-Investigator(Kenkyū-buntansha) HACHISU Tatsuyuki  Center of Shibayagi Chief Room Investigator, 研究室長
HARASAWA Ryou  University of Tokyo Animal Fascility Associate Professor, 医学部・動物実験施設, 助教授 (70159101)
ABE Kenji  National Institute of Health Pathology Chief Investigator, 病理, 主任研究官 (60130415)
輿水 馨  東京大学, 医学部・動物実験施設, 教授 (90011866)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 1991: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1990: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1989: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsWoodchuck / Woodchuck hepatitis virus / Hepatic cancer / PCR / RIA / c-myc / n-myc / 肝炎ウィルス / ウィルスキャリヤ- / 肝臓癌 / 肝炎ウィルスキャリヤ- / C-myc
Research Abstract

Basical problems about conditions of care and breeding woodchucks such as cages, housing or food were solved. We made stainless steel indoor cages(O. 36cm^2 floor space) which are very useful for keeping animals to observe them or make experiment but in this cage they did not mate or breed. In larger indoor iron boxes(2 m^2) animals could breed but new born pups were often killed by their dams. In our conclusion the best circumstances for breeding is outdoor permanent above ground cages in which litter size same as those of wild animals were obtained. Food pellets we produced are same as those of guinea pigs containing less crude fibers.
We succeeded in establishing a method for obtaining artificial virus carriers by injecting virus into non virus carrier pupps. Even in 6 weeks old pupps we could get positive results. We conclude 2-4 weeks old pupps are best to be injected with virus as they are very active with open eyes and mortality is already low. Newly obtained carriers do not still develop cancers. We have checked positivity of hepatitis virus by RIA for surface antigen but we developed new technique utilizing polymerase chain reaction. As primer we used DNA part coding surface antigen.
We followed wild spontaneous carriers for detecting hepatic cnacers and found when volume of cancer become larger than 10cm^3 they develop very rapidly. Integration pattern of virus DNA and n-myc rearrangement suggested that cancer develop both multicentric and metastatic. C-myc rearrangement occur very rare.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] 岡本 信明: "ウッドチャック肝細胞癌におけるCーmyc遺伝子の再編成" 肝臓. 31. 220-221 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 志賀 淳治: "肝癌におけるHBV遺伝子の組み込み" 内科. 66. 642-643 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 河村 美奈: "ウッドチャックに対するGOF麻酔の応用" 獣麻酔外科誌. 20. 57-63 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiga,J.: "Brecding and care for wild woodchucks(marmoto mchax.)by indoor and outdoor housing" Exp.Anim.38. 155-158 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 阿部 賢治: "ウッドチャック肝炎ウィルスの感染実験" 肝臓. 30. 98-99 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 青山 弘: "ウッドチャック肝細胞癌の発生様式" 肝臓. 29. 404-405 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 志賀 淳治: "実験動物の生物学的特性デ-タ" ソフトサイエンス, 656 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okamoto, N.: "Rearrangement of C-myc gene in woodchucks hepatocellular carcinoma" Kanzo. 31(2). 220-221 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiga, J.: "Integration of hepatitis B virus in hepatocellular carcinoma" Niaka. 66(4). 642-643 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamura, M.: "Anesthesia of GOF for woodcucks" Anesth. Vet. Surg.20(3). 57063 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiga, J.: "Breeding and care for wild woodchucks(Marmota monax) by indoor and outdoor housing" Exp. Anim.38(2). 155-158 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe, K.: "Infection experiment of woodchucks" Kanzo. 30(6). 98-99 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Aoyama, H.: "Developmental pattern of hepatocellular carcinoma of woodchuck. Study by echographical follow up of cancer and integration pattern of hepatitis virus" Kanzo. 29(3). 404-405 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe, K.: "Establishment and characterization of a woodchuck hepatocellular carcinoma cell line(WH44KA)" Jpn. J. Cancer Res.79(3). 342-349 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe, K.: "Enzyme-altered liver cell foci in woodchuck infected with woodchuck hepatitis virus" Jpn. J. Cancer Res.79(4). (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiga, J.: "A case report of cysticercosis caused by Cysticercus longicollis, a Larval form of Taenia crassiceps in a woodchuck(Marmota monax)" Exp. Anim.36(2). 213-217 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiga, J.: "Special biological data of experimental animals. Woodchucks" Soft Science. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大友 邦: "肝細胞癌に対する酸化鉄粒子による造影 MRIの有用性-ウッドチャックにおける検討-" 日本医放会誌. 51. 433-435 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiga,J.: "Development and growth pattern of small heputocellular carcinomas in wood chucles" EXP.Anim. 40. 545-548 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 原澤 亮: "PCRの基礎と応用" ビフィズス. 4. 141-144 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 原澤 亮: "PCR法によるウッドチャック肝炎ウィルスの検出" 日仏獣医誌. 2. 48- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiga,J.: "Integration pattern and n-myc rearrangement of woodchucls hepatitis virus DNA in heputocellular carcinoma" Hepatalogy.

    • Related Report
      1991 Annual Research Report
  • [Publications] Imamura,I.: "Quantitative evoluation of parevchymal liver cell volume and total hepatocyte number in civrhatic patients" Hepatalogy. 14. 448-453 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ohtomo,K: "Regenerating nodules of liver clrrhosis:MR imaging with Pathological correlation" ATR. 154. 505-507 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohna,Y: "A histological analysis of five cases of adenomatous hyperplosia containing minute hepatocellular carcinoma" Acta.Path.Jpn. 40. 267-278 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masumi,A: "Hcatーshocls pratein and hypertraphy in the liver of rat infased with methicvin free pareuteral nutrition" Bioch.Biophys.A.1052. 502-508 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shiga,J.: "Development and growth puttern of small hepatocellular carcinoma of woodchuck" Exp.Avim.

    • Related Report
      1990 Annual Research Report
  • [Publications] 志賀 淳治: "肝癌におけるHBV遺伝子の組み込み" 内科. 66. 642-643 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohnishi,S.: "Establishment of a new cell line from a woodchuck hepato cellular carcinoma" Hepatology. 8. 104-107 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shiga,J.: "Breeding and care for wild woodchuck(Marmotei monax)by indoor and outdoor housing" Exp.Anim. 38. 155-158 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Abe,K.: "Enzymealtered liver cell foci in woodchucks infected with woodchucks hepatitis virus" Gann. 79. 466-4 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Abe,K.: "Non-A,non-B hepatitis:uisualization of virus-like particles from chimpanzee and human sera" Arch.Virol.104. 351-355 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 河村美奈: "ウッドチャックに対するGOF麻酔の応用" 獣麻酔外科. 20. 57-63 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 阿部賢治: "ウッドチャック肝炎ウィルスの感染実験" 肝臓. 30. 700-701 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 志賀淳治: "実験動物の生物学的特性デ-タ(分担)" ソフトサイエンス社田嶋嘉雄監修, 549-555 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2019-02-15  

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