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Alternative to breast carcinogenicity test using a tissue culture method

Research Project

Project/Area Number 01880013
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

SUZUKI Kunio  Inst. Phys. Chem. Res., Anim. Cell. Lab. Senior Research Scientist, 動物・細胞システム研究室, 副主任研究員 (60100054)

Co-Investigator(Kenkyū-buntansha) MITSUOKA Tomotari  Inst. Phys. Chem. Res., Frontier Res. Prog., Head, 国際フロンティア研究システム, チームリーダー (30157549)
MIZUTANI Takeo  Inst. Phys. Chem. Res., Lab. Anim. Ctr. Senior Technical Scientist, 動物試験室, 室長 (30087598)
大和田 勉  理化学研究所, 動物試験室, 技師補
尾崎 明  理化学研究所, 動物試験室, 技師 (30087605)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1990: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1989: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsBreast cancer / Carcinogens / Mammary epithelial cell / Micronucleus / Alternative / Mammary epitherial cell
Research Abstract

We developed a new in vitro micronucleus test using cultured mouse mammary epithelial cells as a tissue-specific screen for suspected breast carcinogens.
Mammary epithelial cells from pregnant mice were cultured in serumfree Ham's F-12/Dulbecco's modified Eagle's medium supplemented with insulin, epidermal growth factor, transferin, cholera toxin, bovine serum albumin and antibiotics. Several breast carcinogens and other chemicals were added to the culturds at day 6 of culture, and the number of micronuclei per 1, 000 cells was scored at 24hr after treatment.
In the present study, five breast carcinogens, N-methyl-N-nitrosourea, N-butyl-N-nitrosourea, 7, 12-mdimethylbenz (a) anthracene, 20-methylcholanthrene and 2-acetamidofluorene, and one direct carcinogen, N-methyl-N'-nitro-Nnitrosoguanidine increased the micronuclei incidence in the cultures.
Since all breast carcinogens and a direct carcinogen induced micronuclei in the culture of mouse mammary epitherial cells in a dose related manner, but other agents generally do not, these results justify the use of micronuclei as a short term, in vitro screen of suspected breast carcinogens.

Report

(4 results)
  • 1991 Final Research Report Summary
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serum-free culture conditions" Environmental and Molecular Mutagenesis. 14. 195 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 鈴木 邦夫: "動物の組織および細胞に特異的な発癌物質検出法" バイオインダストリ-. 7. 49-55 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Research. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells groun under serum - free culture conditions" Environm. Mol. Mutagen.14. 195 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kunio Suzuki: "Animal Tissue and cell specific screening test for carcinogens (in Japanese)" Bio Industry. 7. 49-55 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serumーfree culture conditions" Enviromental and Molecular Mutagenesis. 14. 195 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 鈴木 邦夫: "動物の組織および細胞に特異的な発癌物質検出法" バイオインダストリ-. 7. 49-55 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Research. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kunio Suzuki: "In Vitro micronuclei in the mouse mammary epitherial cells ground under serum-free culture conditions" Environm. Mol. Mutagen. 14. 195 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kunio Suzuki: "Animal tissue and cell specific screening test for carcinogens (in Japanese)" Bio Industry. 7. 49-55 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Research. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serum-free culture conditions" Environmental and Molecular Mutagenesis. 14. 195 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 鈴木邦夫: "動物の組織および細胞に特異的な発癌物質検出法" バイオインダストリ-. 7. 49-55 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2019-02-15  

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