| Co-Investigator(Kenkyū-buntansha) |
SCHILPEROORT ライデン大学(オランダ), 教授
QUETIER F. ハ゜リXI大学(フランス), 教授
HANDRO W. サンハ゜ウロ大学(ブラジル), 教授
SCHELL J. マックスプランク研究所(西ドイツ), 教授
PANNIE D. カラカス大学(ベネズエラ), 教授
DODDS J.H. 国際ジャカ゛イモ研究所(ペルー), 研究員
ZENK M.H. ミュンヘン大学(西ドイツ), 教授
LAMB C.J. ソーク研究所(米国), 助教授
TEPFER M. INRAベルサイユ研究所(フランス), 研究員
DE Vries S. ワーゲニンゲン農業大学(オランダ), 教授
STURM T. FMI研究所(スイス), 研究員
TANIMOTO Shizufumi Faculty of Agriculture, Saga University, 農学部, 助教授 (20217152)
ONO Michiyuki Faculty of Textile Science and Technology, Sinshu University, 繊維学部, 助手 (50201405)
KYO Masaharu Faculty of Agriculture, Kagawa University, 農学部, 助手 (70195395)
SHIMOMURA Koichiro National Institute of Hygienic Sciences, 国立衛生試験所, 室長
INOGUCHI Masahiko Faculty of Science, Okayama Science University, 理学部, 助手 (00213190)
SATOU Shinobu Institute of Biological Sciences, University of Tsukuba, 生物科学系, 講師 (70196236)
KAMADA Hiroshi Institute of Biological Sciences, University of Tsukuba, 生物科学系, 助教授 (00169608)
W. Handro San Paulo University, Brazil
J. H. Dodds International Potato Center, Peru
D. Pannie Caracas University, Benezuela
M. Tepfer INRA Versailles, France
M. H. Zenk University of Munchen, Germany
C. J. Lamb Salk Institute, U. S. A.
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1990: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Research Abstract |
Cooperative research between Japan and foreign countries, especially U.S.A. and European countries, was carried out in plant bioscience field.
1. On transformation : Several transformation methods for higher plants have already been developed and some successful results have been reported in model plants. However, only limited number of reports are available on transformation of agriculturally important plants. Therefore, cooperative research was carried out on the transformation of potato and sweet potato. Our results showed that Ri plasmid was an adequate vector for introducing foreign genes such as kanamycine resistant and GUS genes. Studies on transformation of Streptocarpus, an important material for investigating flower bud differentiation, has been continued.
2. On glycoproteins : A carrot secretory glycoprotein GP57 isolated by Tsukuba group was compared with a similar glycoprotein isolated in Wageningen Agricultural University. The expression pattern of the both glycoproteins is comparable, but antiserum for GP57 did not react to the another. The gene expression of GP57 was examined in FMI of Switzerland. The result showed that GP57 gene expression was wound-inducible and thought to have a physiological function in defense mechanism.
3. On control mechanism of gene expression : Expression of agropine synthase gene which was identified on T-DNA of Ti plasmid was controlled by the promoter region of the gene and showed a specificity related to cell division. Homology search on nucleotide sequence among various T-DNA genes was carr
ied out in Leiden University and showed no homology among them.
4. On secondary metabolites : Production of some secondary metabolites is related to tissue and organ differentiation. Tropane alkaloid production was directly related to root differentiation. Detailed examination on synthetic pathway of secondary metabolites was carried out in cooperation and some new results were obtained.
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