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Regulation of Glomerular Ultrafiltration by Glomerular Epithelial Cells

Research Project

Project/Area Number 02044057
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNiigata University

Principal Investigator

YAMAMOTO Tadashi  Niigata University, School of Medicine, Assistant Professor, 医学部, 助教授 (30092737)

Co-Investigator(Kenkyū-buntansha) CURTIS B. WI  The Scripps Reseach Institute, Department, Member
ROLAND C. BL  University of California, San Diego, Depa, Professor
KIHARA Itaru  Niigata University, School of Medicine, Professor, 医学部, 教授 (80018324)
HIROSE Shigehisa  Tokyo Institute of Technology, Dept. of Biological Sciences, Professor, 生命理工学部, 教授 (10134199)
BLANTZ Roland C.  University of California, San Diego, Dept. of Medicine, Professor
WILSON Curtis B.  The Scripps Research Institute , Dept. of Immunology, Member
WILSON Curti  The Scripps Research Institute, Member
BLANTZ Rolan  Department of Medicine, University of Cal, Professor
BLANTS Rolan  University of California, Dept.Medicine, 教授
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1990: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsGlomerular ultrafiltration / Glomerular epithelial cell / Atrial natriuretic peptide / Receptor / mRNA / 腎糸球体 / 限外濾過 / 形態計測法 / マイクロパンクチャ-法 / mRNA
Research Abstract

Glomerular filtration is defined by glomerular ultrafiltration pressure and ultrafiltration coefficicnt. The former has been shown to be regulated by constriction or relaxation of the glomerular afferent and efferent arteries, however how the ultrafiltration is controled in the glomerulus has not been clarified yet. Atrial natriuretic peptide (ANP) is a hormone which facilitates diuresis and natriuresis by increasing the ultrafiltration coefficient and subsequently the glomerular filtration rate and by suppressing sodium reabsorption from collecting ducts. To elucidate glomerular cells which play a role on regulation of glomerular ultrafiltration, mRNA for natriuretic peptide receptors was localized in the kidney by ribonucleasc protection assay and in situ hybridization. Ribonuclease protection assay and in situ hybridization showed that the glomerular epithelial cells have mRNA for type A and type C ANP reccptor subtypes. Since type A receptors but not type C receptors have guanylate cyclase domain in the cytoplasmic region, ANP is predicted to excite the glomerular epithelial cells through binding to the type A receptors and to mediate the increase of ultrafiltration coefficient.
The immunological injury of the glomerular epithelial cells by an in vivo administration of a specific antibody in rats distorted the glomerular filtration function such as glomcrular ultrafiltration coefficient. Morphometric analysis revealed no alterations except an increase in the volume of glomerular cpithclial cclls after the immune injury of these cells. In turn, the ultrafiltration coeffieient decreased when mesangial cells were damaged by in vivo administration of another antibody against these cells. These results indicate that the glomerular cpithclial cells play an important role in the regulation of glomerular filtration through changing ultrafiltration coefficient which might be also regulated by mesangial cells as well.

Report

(3 results)
  • 1992 Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Eishin YAOITA: "Desmin-posiuive epithelial cells outgrowing from rat encapsulated glomeruli" Eur J Cell Biol. 54. 140-149 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiromi HAGEWARA: "Properties of rat uterus endothelin receptor sites" Biomed Res. 11. 93-98 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi YAMAMOTO: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat" Kidney International. 40. 705-713 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi KAWASAKI: "Depletion of CD8 positive cells prevents proteinuria and glomeurlar injury in nephrotoxic serum nephritis of WKY rat" Kidney International. 41. 1517-1526 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi KAWASAKI: "Antibodies against intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 prevent glomerular injury in rat experimental crescentic glomerulonephritis" J Immunol. 150. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Roland C. Blantz: "The role of the mesangial cell in the glomerular response to volume and angiotensin II" Am J Physiol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi YAMAMOTO: "Atrial natriuretic peptide receptor subtypes in the kidney" Nishimura Co.Lud,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiromi Hagiwara: "Immunochemical characterization and localization of endothelin ET receptor" Am J Physiol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takeshi Mizuno: "A variant from of the type C atrial natriuretic peptide receptor generated by alternative splicing" J Biol Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Ymmamoto: "Expression of mRNA for atrial natriuretic peptide receptor subtypes in the kidney"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Roland C. Blantz: "The role of the glomerular epithelial cell in the regulation of glomerular hemodynamics"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Eishi Yaoita: "Desmin-positive epithelial cells outgrowing from rat encapsulated glomeruli" Eur J Cell Biol. 54. 140-149 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 'iromi Hagiwara: "Properties of rat uterus endothelin receptor sites" Biomed Res. 11. 93-98 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat" Kidney International. 40. 705-713 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi Kawasaki: "Depletion of CD8 positive cells prevents proteinuria and glomerular injury in nephrotoxic serum nephritis of rat" Kidney International. 41. 1517-1526 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi Kawasaki: "Antibodies against intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 prevent glomerular injury in rat experimental crescentic glomerulonephritis" J Immunol. 150. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Roland C. Blantz: "The role of the mesangial cell in the glomerular response to volume and angiotensin II" Am J Physiol. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiromi Hagiwara: "Immunochemical characterization and localization of endothelin ET_B receptor" Am J Physiol. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takeshi Mizuno: "A variant form of the type C atrial natriuretic peptide receptor generated by alternative RNA splicing" J Biol Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Expression of mRNA for atrial natriuretic peptide receptor subtypes in the kidney"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Roland C. Blantz: "The role of the glomerular epithelial cell in the regulation of glomerular hemodynamics"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: Nishimura Co. Ltd. Atrial natriuretic peptide receptor subtypes in the kidney,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat" Kidney International. 40. 705-713 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Participation of cytokines in proliferation of mesangial cell" Acta Med.Biol.39. 87-91 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Matsuki: "Interleukin-1 mRNA-expressing macrophages in human chronically inflamed gingival tissues" Am.J.Pathol.138. 1299-1305 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Katsutoshi Kawasaki: "Depletion of CD8 positive cells prevents proteinuria and glomerular injury in nephrotoxic serum nephritis of WKY rat" Kidney International.

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Immunohistochemical localization and mRNA expression of atrial natriuretic peptide type C receptor in the kidney"

    • Related Report
      1991 Annual Research Report
  • [Publications] Eishin Yaoita: "Desminーpositive epithelial cells outgrowing from rat encapsulated glomeruli" Eur J Cell Biol. 54. 140-149 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiromi Hagiwara: "Properties of rat uterus endothelin receptor sites" Biomed Res. 11. 93-98 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Immunohistochemical and immunoelectron microscopic localization of atrial natriuretic peptide receptor on glomerular epithelial cells"

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat"

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Systemic localization of type C receptor for atrial natriuretic peptide by immunohistochemistry using monoclonal antibodies"

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Gene expression and immunohistochemical localization of type C receptor for atrial natriuretic peptide on pulmonary endothelial cells"

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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