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Studies on Biochemical Functions of Pyrroloquinoline Quinone

Research Project

Project/Area Number 02044106
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionFaculty of Agriculture, Yamaguchi University

Principal Investigator

ADACHI Osao  Professor, Faculty of Agriculture, Yamaguchi University, 農学部, 教授 (20027189)

Co-Investigator(Kenkyū-buntansha) P.POSTMA  Associate Professor, Delft University of Technology, 助教授
G.BAREND  Technician, Delft University of Technology, 技官
J.JONGEJAN  Associate Professor, Delft University of Technology, 助教授
J.FRANK  Associate Professor, Delft University of Technology, 助教授
J Duine  デルフト工科大学, 教授
TAKIMOTO Kohichi  Professor, Faculty of Agriculture, Yamaguchi University, 農学部, 教授 (00115875)
YAMADA Mamoru  Associate Professor, Faculty of Agriculture, Yamaguchi University, 農学部, 助教授 (30174741)
SHINAGAWA Emiko  Associate Professor, Ube Technical College, 助教授 (20116726)
AMEYAMA Minoru  Professor, Faculty of Engineering, Kansai University, 工学部, 教授 (90022053)
TOYAMA Hirohide  Research Associate, Faculty of Agriculture, Yamaguchi University, 農学部, 助手 (60240884)
MATSUSHITA Kazunobu  Associate Professor, Faculty of Agriculture, Yamaguchi University, 農学部, 助教授 (50107736)
J.A.DUINE  Professor, Delft University of Technology
H.SCHROVER  Postgraduate student, Delft University of Technology
SCHROVER H.  デルフト工科大学, 大学院生
BAREND G.  デルフト工科大学, 技官
JOGEJAN J.A  デルフト工科大学, 助教授
FRANK J.  デルフト工科大学, 助教授
DUINE J.A  デルフト工科大学, 教授
SCHROVER H  デルフト工学大学, 大学院生
GROEN B.W.  デルフト工科大学, 技官
JONGEJAN J.A  デルフト工科大学, 助教授
FRANK.J.  デルフト工科大学, 助教授
DUINE J.A.  デルフト工学大学, 教授
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥19,600,000 (Direct Cost: ¥19,600,000)
Fiscal Year 1992: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1991: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1990: ¥6,600,000 (Direct Cost: ¥6,600,000)
KeywordsPyrroloquinoline quinone / quinoprotein / methanol dehydrogenase / PQQ-dependent alcohol dehydrogenase / ピロロキノリンキノン(PQQ) / メタノ-ル脱水素酵素 / アルコ-ル脱水素酵素 / グルコ-ス脱水素酵素 / エタノ-ル脱水素酵素 / チトクロムC
Research Abstract

The quinoprotein dehydrogenase has been classified into four groups according to their localization and characterization :
(1) methanol dehydrogenase in methylotrophs,
(2) quinoprotein alchold dehydrogenase from non-methylotrophic bacteria, when they are grown on ethanol as sole carbon source.
(3) Quinohemoprotein alcohol dehydrogenase is formed as apo-enzyme in Commamonas testosteroni, while Pseudomonas putida forms holo-enzyme when they are grown on primary alcohols as the carbon source.
(4) Quinohemoprotein alcohol dehydrogenase-cytochrome c complex is formed is formed in the cytoplasmic membranes of acetic acid bacteria.
During the course of this research program, the following new findings have been added to clear up the function of pyrroloquinoline quinone and quinoproteins.
(1) Membrane-bound, pyrroloquinoline quinone-dependent, alcohol dehydrogenase functions as the primary dehydrogenase in the respiratory chain of acetic acid bacteria. An ability of the enzyme to directly react with … More ubiquinone was investigated in alcohol dehydrogenases purified from both Acetobacter aceti and Gluconobacter suboxydans. The reconstitution of alcohol oxidase was done by binding the detergent-free dehydrogenase at room temperature to proteoliposomes that had been prepared in advance from a ubiquinol oxidase and phospholipids containing ubiquinone by detergent dialysis using octyl-glucoside. The proteoliposomes thus reconstituted had a reasonable level of ethanol oxidase activity, the electron transfer reaction of which was also able to generate a membrane potential.
(2) Methanol and ethanol oxidase respiratory chains of the methylotrophic acetic acid bacterium, Acetobacter methanolicus, was investigated. In this study, an 32 KDa peptide was found in alternative type of methanol dehydrogenase which contained three different subunits two of which corresponded to alpha-subunit and beta-subunit. The third subunit was finally concluded to be the MoxJ product in methanol oxidase, because the N-terminal amino acid sequence of the peptide showed a high homology to that of MoxJ gene from Paracoccus denitrificans or Methylobacterium extorquens AMl.
(3) Pseudomonas putida HK5, isolated from soil and identified thereafter, produced three different quinoprotein alcohol dehydrogenase as holo-enzyme. When grown on ethanol, it produced a quinohemoprotein alcohol dehydrogenase, While grown on glycerol, another quinohemoprotein alcohol dehydrogenase which can oxidize glycerol. The third type of quinoprotein alcohol dehyderogenase was similar to those found in non-methylotrophic bacteria which were grown on ethanol. The third alcohol dehydrogenase contained no heme component and was not able to oxidize alcohol with potassium ferricyanide as an electron acceptor. Less

Report

(3 results)
  • 1992 Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 松下 一信,高木 善弘,品川 恵美子,飴山 實,足立 収生: "酢酸菌のアルコール酸化素呼吸〓" 日本農芸化学会欧文誌. 56. 304-310 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Meulerbeng,Sellink,Reigman Postoma: "KlebsiellaのPQQオペロンの構造" 分子遺伝学雑誌. 232. 284-294 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 松下 一信,高橋 和宏,高橋 源尚,飴山 實,足立 収生: "メタノール資化性酢酸菌のメタノール及びエタノール酸化呼吸〓" 日本生化学会欧文誌. 111. 739-747 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 久野 智広,村田 幸作,木村 光,松下 一信,外山 博英,足立 収生: "Citrobacter freundiiの膜佶合型スペルミジン脱水素酵素の性質" 日本農芸化学会欧文誌. 56. 1916-1920 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 山田 守,浅岡 伸太,H,SAIER,山田 康枝: "大腸菌のgcd遺伝子の性質とその発現調節" 米国微生物学会誌. 175. 568-571 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 松下 一信,中村 淳雄,外山 博英,足立 収生,G.BAREUD,J.A.DUINE: "Comamonas testosteroniの可溶性及び膜佶合型キノヘモプロテインADHの構造" 米国生化学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 松下 一信,足立 収生: "Principlesand Applications & Quinoproteins(分担)" Marcel DekRer Iuc., 37 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] J.A.DUINE,J.A.JONGEJAN: "Bioinorganic Catalysis(分担)" Marcel DekRer, (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Matsushita, Y. Takaki, E. Shinagawa, M. Ameyama, O. Adachi: "Ethanol oxidase respiratory chain of acetic acid bacteria. Reactivity with ubiquinone of pyrroloquinoline quinone-dependent alcohol dehydrogenases purified acetic acid bacteria." Biosci. Biotech. Biochem.56. 304-310 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] J. Meulenberg, E. Sellink, N. Reigman, P. Postma: "Nucleotide sequence and structure of the Klebsiella pneumonia PGG operon." Mol. Gen. Genet.232. 284-294 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Matsushita, K. Takahashi, M. Takahashi, M. Ameyama, O. Adachi: "Methanol and ethanol oxidase respiratory chains of the methyloptrophic acetic acid bacterium, Acetobacter methanolicus." J. Biochem.111. 739-747 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Hisano, K. Murata, A. Kimura, K. Matsushita, H. Toyama, O. Adachi: "Characterization of membrane-bound spermidine dehydrogenase of Citrobacter freundii." Biosci. Biotech. Biochem.56. 1916-1920 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Yamada, S. Asaoka, M. Saier, Y. Yamada: "Characterization of the gcd gene from Escherichia coli k-12 W3110 and regulation of its expression." J. Bacteriol.175. 568-571 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Ameyama, K. Matsushita, E. Shinagawa, O. Adachi: Academic Press. Biochemical and physiological functions of pyrroloquinoline quinone in Vitamins and Hormones, 229-270 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Matsushita, O. Adachi: Marcel Dekker. Bacterial quinoprotein glucose dehydrogenase and alcohol dehydrogenase in Principles and Applications of Quinoproteins, 47-63 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Hamagishi,S.Murata,H.Kamei,T.Oki,Osao Adachi and Minoru Ameyama: "New Biological Properties of Pyrroloquinoline Quinone and Its Related Compounds:Inhibition of Chemiluminescence,Lipid Peroxidation and Rat Paw Edema" Journal of Pharmacology and Experimental Therapeutics. 255. 980-985 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazunobu Matsushita,Y.Nagatani,Emiko Shinagawa,Osao Adachi & Minoru Ameyama: "Reconstitution of the Ethanol Oxidase Respiratory Chain in Membranes of Quinoprotein Alcohol Dehydrogenase-Deficient Gluconobacter Suboxydans subsp.α Strains" Journal of Bacteriology. 173. 3440-3445 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Johannis A.Duine: "Quinoproteins:enzymes containing the quinoid cofactor pyrroloquinoline quinone,topaquinone or tryptophan-tryptophan quinone" European Journal of Biochemistry. 200. 271-284 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] P.W.van Ophem,G.Euverink,L.Dijkhuizen & Johannis A.Duine: "A novel dey-linked a lcohol dehydrogenaese activity present in some Gram-Positive bacteria" FEMS Microbiology Letters. 80. 57-64 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazunobu Matsushita,Y.Takaki,Emiko Shinagawa,Minoru Ameyama & Osao Adachi: "Ethanol Oxidase Respiratory Chain of Acetic Acid Bacteria.Reactivity with Ubiquinone of Pyrrologuinoline Quinone-dependent Alcohol Dehydrogenases Purified from Acetobacter aceti and Gluconobacter suboxydans" Bioscience,Biotechnology and Biochemistry. 56. 304-310 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Hisano,K.Murata,A.Kimura,Kazunobu Matushita & Osao Adachi: "Further Properties of Spermidine Dehydrogenase from Citrobacter freundii IFO 126861" Bioscience,Biotechnology and Biochemistry. 56. 311-314 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Minoru Ameyama,Kazunobu Matsushita,Emiko Shinagawa,& Osao Adachi: "Biochemical and Physiological Functions of Pyrroloquinoline Quinone in "Vitamins and Hormones" Vol.46" Academic Press,Inc.Harcourt Jovanovich Publishers,New York, 229-270 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Minoru Ameyama & Osao Adachi: "Biochemical and Physiological Functions of Pyrroloquinoline Quinone in "Enzymes Dependent on Pyridoxal Phosphate and Other Carbonyl Compounds as Cofactors"" Pergamon Press, 465-473 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Emiko Shinagawa,Kazunobu Matsushita,Osao Adachi & Minoru Ameyama: "Evidence for Electron Transfer via Ubiquinone between Quinoproteins DーGlucose Dehydrogenase and Alcohol Dehydrogenase of Gluconobacter suboxydans" Journal of Biochemistry. 107. 863-867 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Osao Adachi,Kazuyuki Okamoto,Kazunobu Matsushita,Emiko Shinagawa & Minoru Ameyama: "An Enzyme Catalyzing Liberation of Pyrroloquinoline Quinone (PQQ) from PQQーAdducts" Agricultural and Biological Chemistry. 54. 2481-2483 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Osao Adachi,Kazunobu Matsushita,Emiko Shinagawa & Minoru Ameyama: "Calcium in Quinoprotein Methanol Dehydrogenase Can Be Replaced by strontium" Agriculture and Biological Chemistry. 54. 2833-2837 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Osao Adachi,Kazunobu Matsushita,Emiko Shinagama & Minoru Ameyama: "Purification and Properties of Methanol Dehydrogenase and Aldehyde Dehydrogenasefrom Methylobacillus glycogenes" Agricultural and Biological Chemistry. 54. 3123-3129 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.van der Meer,B.Groen,J.Jongejan & J.A.Duine: "The redoxーcycling assay is not suited for the detection of pyrroloquinoline quinone in biologicao samples" FEBS Letters. 261. 131-134 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] E.Karhunen,MーL.NikuーPaavola,L.Viikari,T.Haltia,R.van der Meer,& J.A.Duine: "A novel combination of prosthetic groups in a fungal laccase;PQQ and two copper atoms" FEBS Letters. 267. 6-8 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.A.Duine & J.Jongejan: "Pyrroloquinone Quinone:A Novel Cofactor in “VITAMINS AND HORMONES" Vol.45" Academic Press Inc., 223-262 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Frank & J.A.Duine: "Cytochrome c_L and Cytochrome c_H from Hyphomicrobium X in “METHODS IN ENZYMOLOGY" Vol.188" Academic Press inc., 303-308 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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