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Molecular polymorphism, gene regulation and adaptive evolution of duplicated amylase genes in Drosophila.

Research Project

Project/Area Number 02044113
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKyusyu University

Principal Investigator

YAMAZAKI Tsuneyuki  Kyushu University, Faculty of Science, Professor, 理学部, 教授 (10108649)

Co-Investigator(Kenkyū-buntansha) WINIFRED Doane  Arizona State University, Professor, 教授
HICKEY D.  オタワ大学, 教授
CHUCK Langley  California University, Professor, 教授
PHIL Hedrick  Pennsylvania State University, Professor, 教授
LEWONTIN R.C  ハーバード大学, 教授
D. Hickey  Ottawa University, Professor
R. C. Lewontin  Harvard University, Professor
DOANE Winifr  アリゾナ州立大学, 教授
LANGLEY Chuk  カリフォルニア大学, 教授
HEDRICK Phil  ペンシルバニア州立大学, 教授
LANGLEY Chuc  カリフォルニア大学, 教授
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1990: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsIsozyme polymorphism / Amylase / Duplicated genes / Concerted evolution / Drosophila / gene regulation / Inducibility / 調節領域
Research Abstract

DNA sequences of several polymorphic isozymes at duplicated amylase genes were determined in Drosophila melanogaster and its sibling species. Large differences in base sequences between different allozymes compared with identical allozymes indicated long history of different isozymes. In addition, differences between the two duplicated amylase genes within species were much smaller than those of the equivalent locus between different species, indicating the existence of concerted evolution between duplicated genes within species the evidence of concerted evolution was observed only in the coding region but not in the spacer regions. Theoretical study is still under way, concerning the molecular mechanism of this phenomenon. We also cloned a transfacting regulatory gene on the X-chromosome, which appears to affect the inducibility of amylase. The function of this gene is being investigated using P-element mediated transformation technique.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Mukai,T.,K.Harada,Kusakabe and T.Yamazaki: "Estimation of Spontaneous mutation rates at enzyme loci in Drosophila melanogaster." Proc.Natl.Acad.Sci.USA. 66. 29-32 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka,E.and T.Yamazaki: "A novel eye morphology induced by P element in somatic tissue of Drosophila melanogaster." Molecular and General Genetics. 227. 181-186 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka,E.and T.Yamazaki: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet.Res.Camb.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka,E.,M.M.Green,and T.Yamazaki: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster." Proc.Natl.Acad.Sci.USA. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hawkley,S.A.,W.W.Doane and R.A.Norman: "Molecular analysis of cls-regulatory sequenses at the α-amylase locus in Drosophila melanogaster." Genetics. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Doane,W.W.,D.S.Thompson R.A.Norman & S.A.Hawkley: "Molecular genetics of a three-gene cluster in the Amy region of Drosophila." Isozymes. 344. 19-48 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamazaki,T.: "POPULATION BIOLOGY OF GENES AND MOLECULES (Nucleotide polymorphism and evolution of multigine families in Drosophila.)" ed.by N.Takahata and J.F.Crow (Baifukan), 151-167 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka,E.,and T.Yamazaki: "NEW ASPECT OF GENETICS IN MOLECULAR EVOLUTION (Genetic variation and regulation of P elements in Drosophila melanogaster.)" ed.by Kumura,M.and T.Takahata (japan Sci.Press), 1-14 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mukai, T., K. Harada, Kusakabe and T. Yamazaki: "Estimation of Spontaneous mutation rates at enzyme loci in Drosophila melanogaster." Proc. Natl. Acad. Sic. USA.66. 29-32 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka, E. and T. Yamazaki: "A novel eye morphology induced by P element in somatic tissue of Drosophila melanogaster." Molecular and General Genetics.227. 181-186 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka, E., and T. Yamazaki.: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet. Res. Camb.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka, E., M. M. Green and T. Yamazaki: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster." Proc. Natl. Acad. Sci. USA.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hawkley, S. A., W. W. Doane and R. A. Norman: "Molecular analysis of cls-regulatory sequences at the alpha-amylase locus in Drosophila melanogaster." Genetics. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamazaki, T.: N. Takahata and J. F. Crow, Baifukan. Population biology of genes and molecules., 151-167 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nitasaka E. and T. Yamazaki: Kimura, M. and T. Takahata, Japan Sci. Press.New aspect of genetics in molecular evolution, 1-14 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mukai,T.,K.Harada.Kusakabe and T.Yamazaki: "Estimation of Spontaneous mutation rates at enzyme loci in Drosophila melanogaster." Proc.Natl.Acad.Sci.USA. 66. 29-32 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nitasaka,E.and T.Yamazaki: "A novel eye morphology induced by P element in somatic tissue of Drosophila melanogaster." Molecular and General Genetics. 227. 181-186 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nitasaka,E.and T.Yamazaki: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet.Res.Camb.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nitasaka,E.,M.M.Green,and T.Yamazaki: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster." Proc.Natl.Acad.Sci.USA. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hawkley,S.A.,W.W.Doane and R.A.Norman: "Molecular analysis of clsーregulatory sequenses at the αーamylase locus in Drosophila melanogaster." Gnetics. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Doane,W.W.,D.S.Thompson R.A.Norman & S.A.Hawkley: "Molecular genetics of a three-gene cluster in the Amy region of Drosophila." Isozymes. 344. 19-48 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamazaki,T.: "POPULATION BIOLOGY OF GENES AND MOLECULES(Nucleotide polymorphism and evolution of multigine families in Drosophila.)" ed.by N.Takahata and J.F.Crow(Baifukan), 151-167 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nitasaka,E.,and T.Yamazaki: "NEW ASPECT OF GENETICS IN MOLECULAR EVOLUTION(Genetic variation and regulation of P elements in Drosophila melanogaster.)" ed.by Kimura,M.and T.Takahata(Japna Sci.Press), 1-14 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mukai,T.,K.Harada,Kusakabe and T.Yamazaki: "Estimation of Spontaneous mutation rates at enzyme loci in Drosophila melanogaster" Proc.Natl.Acad.Sci.USA. 66. 29-32 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tanaka,T.,and T.Yamazaki: "Fitness and its components in Drosophila melanogaster." Jpn.J.Genet.65. 417-426 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nitasaka,E.and T.Yamazaki: "A novel eye morphology induced by P element in somatic tissue of Drosophila melanogaster." Molecular and General Genetics.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nitasaka,E.,and T.Yamazaki: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet.Res.Camb.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nitasaka,E.,M.M.Green,and T.Yamazaki: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster." Proc.Natl.Acad.Sci.USA.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamazaki,T.: "POPULATION BIOLOGY OF GENES AND MOLECULES (Nucleotide polymorphism and evolution of multigine families in Drosophira.)" ed.by N.Takahata and J.F.Crow(Baifukan), 00151-167 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nitasaka,E.,and T.Yamazaki: "NEW ASPECT OF GENETICS IN MOLECULAR EVOLUTION(Genetic variation and regulation of P elements in Drosophila melanogaster.)" ed.by Kimura,M.and T.Takahata(Japan Sci.Press), 001-14 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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