| Project/Area Number |
02044130
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Keio Gijuku University |
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Principal Investigator |
NISHIKAWA Takeji Keio University School of Medicine, Professor of Dermatology, 医学部・皮膚科, 教授 (50051579)
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| Co-Investigator(Kenkyū-buntansha) |
AMAGAI Masayuki National Institute of Health, National Cancer Institute, Dermatology Branch, Res, 皮膚科, リサーチ:フェロー (90212563)
STANLEY John R. National Institute of Health, National Cancer Institute, Dermatology Branch, Sen, 皮膚科, 上級研究員
EADY Robin A. J. University of London, Institute of Dermatology, Professor of Cell Pathology, & S, 国立皮膚病研究所・細胞病理部, 主任教授
SHIMIZU Hiroshi Keio University School of Medicine, Instructor of Dermatology, 医学部・皮膚科, 助手 (00146672)
HASHIMOTO Takashi Keio University School of Medicine, Assistant Professor of Dermatology, 医学部・皮膚科, 講師 (20129597)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | hereditary and familial bullous diseases / autoimmune bullous diseases / bullous pemphigoid / immunoelectron microscopy / immunoblotting / immunoprecipitation / 抗表皮基底膜部抗体 / 線状IgA皮膚症 |
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| Research Abstract |
There are a number of skin diseases in which epidermal basement membrane zone (BMZ) is affected. Therefore, the research for epidermal BMZ is important for both diagnostic and therapeutic respects. First, we investigated a series of antibodies against various components of BMZ, including LH7.2 and GB3 monoclonal antibodies and human monoclonal antibody against 23OkD bullous pemphigoid (BP) antigen. One approach is the precise localization of the antigens for these antibodies. For this purpose we have established cryofixation and cryosubstitution for immunoelectron microscopy in collaboration with Professor Eady, England.
Another approach is the determination of such antigens by using biochemical and molecular biological techniques. We discussed for these projects with Drs. Stanley and Amagai, U. S. A. and with Dr. Black, England, who also provided us with sera from their BP cases. Using immunoblot and immunoprecipitation method, we first showed that autoantigen profile is almost the same among Japanese, British and U. S. cases. We also found that although 230kD BP antigen is restricted to BMZ, 170kD BP antigen may exist in both BMZ and cell membrane of basal keratinocytes. Furthermore, although 170kD BP antigen was thought not to be detectable by immunoprecipitation, we showed 170kD BP antigen can be detectable by our improved immunoprecipitation method. In addition, we also established ELISA method using recombinant antigen protein produced by CDNA clone for 230kD BP antigen. This method can substitute the conventional immunofluorescence technique to detect anti-BMZ antibodies in BP sera.
Another important disease is linear IgA bullous dermatosis (LAD). We have investigated for LAD autoantigen as collaboration work with Professor Zone, U. S. A. and Drs. Black and Wojnarowska, England. We confirmed that some LAD sera specifically react with 97kD protein. However, the real nature of this 97kD protein should be clarified by further confirmative studies.
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