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Toxicological Study of Fusarium moniliforme

Research Project

Project/Area Number 02044169
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNational Institute of Health

Principal Investigator

KUMAGAI Susumu  National Institute of Health, Department of Biomedical Research of Foods, 食品衛生部, 部長 (60109965)

Co-Investigator(Kenkyū-buntansha) NORREED W. P.  USDA, Agricultural Research Service, Richard B. Russell Agricultural Research Ce, Richard B. Russell農業研究センター, Research L
IWAKI Masaaki  National Institute of Health, Department of Biomedical Research on Foods, 食品衛生部, 研究官 (20176530)
KASUGA Fumiko  National Institute of Health, Department of Biomedical Research on Foods, 食品衛生部, 研究官 (40183777)
ITOH Yoshinori  National Institute of Health, Department of Biomedical Research on Foods, 食品衛生部, 研究官 (00168387)
KAMIMURA Hisashi  Tokyo Metoropolitan Research Laboratory of Public Health, 生活科学部, 主任研究員
ITINOE Masakatu  National Institute of Hygienic Sciences. Division of Microbiology, 衛生微生物部, 室長 (30132880)
NORRED M.P.  USDA, Richard B・Russell Agricultural Rese, Research L
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1991: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1990: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsFusarium moniliforme / fumonisin / toxicity / sphingolipid / 毒素 / カビ / スフィンゴ脂質 / Fusarium moniliforme / カビ毒 / ウマ白質脳症
Research Abstract

A series of toxicological studies of Fusarium moniliforme was conducted in this project.
1. Contamination of various agricultural products with F. moniliforme and relating species was studied. Imported US corn was heavily contaminated with F. moniliforme. Mainly Fusarium species other than F. moniliforme were isolated from domestic barley and wheat : F. moniliforme was isolated only from one out of 10 wheat samples. A few of pearl barley samples imported from China and Thailand were contaminated with F. moniliforme.
Study of distribution of Fusarium species in soil showed the presence of F. moniliforme and F. subglutinans only in soil of sugarcane farm.
2. Method of analysis of fumonisin, one of the toxins produced by F. moniliforme, was developed and established. Determination of the toxin concentration in the US corn with this method revealed heavy contamination. This method also enabled us to study the toxin production by the strains of the F. moniliforme and F. moniliforme var. subgluticans isolated from domestic agricultural products and soil. Several strains showed very high producibility of fumonisin B1 and B2.
3. Toxicity of cultural extracts and their column fractions of F. moniliforme isolated in this project was examined by using various mammalian cultured cells, primary cultured cees and experimental animals. The result suggested possible presence of a new toxin.
4. Biochemical study of toxic effects of fumonisin at the cellular level demonstrated that the toxin inhibited the sphingolipid biosynthesis through inhibition of sphingosine and sphinganine N-acetyltransferase activity.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kasuga F.,Inoeu S.,Asano T.& Kumagai S.: "Effects of isopropanol on development of inflammatory reactions in rats" Food Chemical Toxicology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Wang E.,Norred W.P.,Bacon C.W.,Riley R.L.,& Merrill A.: "Inhibition of sphingolipid biosynthesis by fumonisins.Implications for diseases associated with Fusarium miniliforme." Journal of Biological Chemistry. 266. 14486-90 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noreed W.P.,Bacon C.W.,Plattner R.D.& Vesonder R.F.: "Differential cytotoxicity and mycotoxin content among isolates of Fusarium moniliforme." Mycopathologia. 115. 37-43 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ito Y.,Ichinoe M.,Igimi S.,Iwaki M.& Kumagai S.: "Toxicity of culture extracts of Fusarium moniliforme isolated from soil in Japan."

    • Related Report
      1991 Annual Research Report
  • [Publications] 一戸 正勝: "Fusarium属の分離と分類・同定" 防菌防徽誌. 18. 399-406 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Ikebuchi,R.Teshima,K.Hirai,M.Sato,M.IChinoe and T.Terao: "Production and characterization of monoclonal antibodies to nivalenol tetraacetate and their application to enzymelinked immunoassay of nivalenol." Biol.Chem.Hoppeーseyler. 371. 31-36 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Teshima,K.Hirai,M.Sato,H.Ikebuchi,M.Ichinoe and T.Terao: "Radioimmunoassay of nivalenol in berley" Appl.Environ.Microbiol.56. 764-768 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Teshima,M.Kawase,T.Tanaka K.Hirai,M.Sato,J.Sawada,H.Ikebuchi,M.Ichinoe & T.Terao: "Production and characterization of a specifiソ monoclonal antibody against mycotoxin zearalenone." J.Agric.Food Chem.38. 1618-1622 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] W.P.Norred et al.: "Hepatotoxicity and renal toxicity in rats of corn samples associated with field cases of equine leukoencephalomalacia." Food Chem.Toxicol.27. 89-96 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] W.P.Norred et al.: "Effects of F.moniliforme and corn associated with equine leukoencephalomalacia on rat neurotransmitters and metabolites." P.S.E.B.M. 194. 265-269 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 上村 尚: "カビ毒分析法の動向" フ-ドケミカル. 5. 99-105 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] 上村 尚: "市販食品のカビ毒汚染実態およびカビ毒除去法における最近の知見" フ-ドケミカル. 5. 88-98 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kamimura H.: "Removal of Mycoroxins During Food Processing" Bioactive Molecules Elsevier. 10. 169-176 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] 上村 尚: "食品のカビとカビ毒" 都薬雑誌. 11. 33-39 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 上村 尚: "インドネシアにおけるアフラトキシンの汚染の実態と摂取量" マイコトキシン. 31. 21-25 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 上村 尚: "カビ毒(マイコトキシン)汚染" 臨床栄養. 78. 148-154 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Iwaki,M,Kitagawa,T.,Akamatsu,Y.,and Aibara,K.: "Cytotoxic effects of aflatoxin B1 and its association with cellular components in chicken embryo primary cultured cells." Biochim.Biophys.Acta. 1035. 146-153 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Iwaki,M.,Okahashi,N.,Takanashi,I.,Kanamoto,T.,Konishi,Y.,Aibara,K.,and Koga,T.: "Expression of a surface antigen gene from serotype c Streptococcus mutans in Streptococcus lactis." Infection and Immunity. 58. 2929-2934 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Koga,T.,Okahashi,N.,Takahashi,I.,Kanamoto,T.,Asakawa,H.,and Iwaki,M.: "Surface hydrophobicity,adherence,and aggregation of cellーsurface protein antigen mutants od Streptococcus mutans serotype c." Infection and Immunity. 58. 289-296 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagahara A.& Kumagai S.: "Excretion and Diatribution of Nitriteーtreated or ーuntreated 1ーMethylー1,2,3,4ーtetrahydroーbーcarbolineー3ーcarboxilic acids in Rats." Food and Chemical Toxicology. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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