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Electron Transfer Reactions of Proteins and Their Applications in Bioelectrochemistry

Research Project

Project/Area Number 02045033
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionKumamoto University

Principal Investigator

TANIGUCHI Isao  Kumamoto University, Professor, 工学部, 教授 (90112391)

Co-Investigator(Kenkyū-buntansha) JAN F. CHELE  Virginia Commonwealth大学, 生物化学科, 教授
JAMES TURNER  Virginia Commonwealth大学, 化学科, 準教授
FRED M. HAWK  Virginia Commonwealth大学, 化学科, 教授
KIDA Kenji  Kumamoto University, Associate Professor, 工学部, 助教授 (00195306)
SAKAKI Shigeyoshi  Kumamoto University, Professor, 工学部, 教授 (20094013)
HAWKRIDGE Fred M.  Virginia Commonwealth University, Professor
TURNER James  Virginia Commonwealth University, Associate Professor
CHELEBOWSKI Jan F.  Virginia Commonwealth University, Professor
CHELEBOWSKI ジヤン  Virginia Commonwealth大学, 生物化学科, 教授
TURNER James  Virginia Commonwealth大学, 化学科, 準教授
HAWKRIDGE Fr  Virginia Commonwealth大学, 化学科, 教授
CHLEBOWSKI F  Virginia Commonwealth大学, 生物化学科, 準教授
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsCytochrome c / Myoglobin / Ferredoxin / Electron transfer reaction / Functional modified electrode / In situ spectroelectrochemical technique / Polypeptide / Cell membrane model / 電子移動反応 / 分光電気化学法 / Inーsitu測定 / ポリーLーリジン / 電気化学酵素触媒反応 / タンパク質 / 分光電気化学 / 構造変化 / ダイナミクス / 呼吸鎖モデル
Research Abstract

In the present research project, electron transfer reactions of proteins and their applications have been studied.
Electron-transfer reactions of three components (purified, deamidated and oligomeric) of cytochrome c obtained in the purification procedure have been examined at both Io_2O_3 and promoter modified electrodes to clarify the role of the purification procedure of cytochrome c for the rapid electron transfer. Promoter-modified electrodes have also been applied to study effects of origin and modification of lysine residues of cytochrome c on the electrode reaction and protein-protein interactions.
Myoglobin (from horse heat, Mb) has found to show a stable redox wave at a clean In_2O_3 electrode, for the first time. The heterogeneous electron-transfer rate constant(k^<O'>)depended on the pH of the solution, and >3 x 10^<-4> cm/s was obtained at pH 6.5 as the best value. The largest k^<O'> value was again observed for sperm whale Mb at pH 6.5 independent of the pI values of Mb used(horse heart : 6.8, sperm whale : 8.25).
For ferredoxin (from spinach and maize, Fd), well-defined redox wave was found to be observed at functional electrodes in the presence of small amounts of polycations, such as poly-L-lysine and poly-L-ornithine. The k^<O'> value was estimated to be >5 x 10^<-3> cm/s. The redox wave was stable in the pH 6 to 10 region. Electrochemistry of Fd was applied to develop bioelectrochemical systems using Fd as an electron-donating mediator for suitable enzyme reactions as in the photosynthetic systems in nature.
Also, in the present research project, new spectroelectrochemical techniques have been developed and applied to study the electron transfer reactions of biological molecules, and a model of cell membrane having a function of electron transport was developed using a Meldola's blue incorporated decanethiol modified gold electrode..

Report

(3 results)
  • 1992 Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] TANIGUCHI,Isao: "New Simple Cell for In situ Fluoroelectrochemical Measurements" Chemistry Letters. 1992. 1217-1220 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Direct electron transfer of horse heart myoglobin at an indium oxide electode" Journal of Electroanalytical Chemistry. 333. 331-338 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KING,Bertha C.: "Electrochemistry of Myoglobin" Review of Polarography(Kyoto). 38. 7-10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Heterogeneous Electron Transfer Reactions of Proteins" Review of Polarography(Kyoto). 38. 17-18 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TOMINAGA,Masato: "Application of Promoter Modified Electrodes Bioelectrochemical Measurements on the Effects of Origin and Modification of Lysine Residues of Cytochrome c" Analytical Sciences. 8. 829-836 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of Cytochrome c Components at Indium Oxide and Promoter Modified Electrodes" Denki Kagaku. 60. 1043-1049 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Direct Electron Transfer of Ferredoxin at an Indium Oxide Electrode in the Presence of Poly-L-lysine" Denki Kagaku. 61. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of myoglobin and cyanomyoglobin from different origins" Journal of Electroanalytical Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Direct electron transfer of ferredoxins at various electrodes in the presence of Polycations" Bioelectrochemistry and Bioenergetics.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 谷口 功: "化学工業(特集号:高機能化する電極とその応用)「分子修飾による電極の機能制御」" 化学工業社, 7 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Proceedings of Fifth International Symposium on Redox Mechanisms and Interfacial Properties of Molecules of Biological Importance(Direct Electron Transfer Reactions of Proteins at Functional Electrodes)" The Electrochemical society,Inc., 12 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hawkridge,Fred M.: "Proceedings of Fifth International Symposium on Redox Mechanisms and Interfacial Properties of Molecules of Biological Importance (Myoglabin Electron Transfer and Ligand Binding Reactions)" The Electrochemical Society,Inc.,12 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: "New Simple Cell for In situ Fluoroelectrochemical Measurements" Chemistry Letters. 1992. 1217-1220 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: "Direct electron transfer of horse heart myoglobin at an indium oxide electrode" Journal of Electroanalytical Chemistry. 333. 331-338 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KING, Bertha C.: "Electrochemistry of Myoglobin" Review of Polarography (Kyoto). 38. 7-10 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: "Heterogeneous Electron Transfer Reactions of Proteins" Review of Polarography (Kyoto). 38. 17-18 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TOMINAGA, Masato: "Application of Promoter Modified Electrodes to Bioelectrochemical Measurements on the Effects of Origin and Modification of Lysine Residues of Cytochrome c" Analytical Sciences. 8. 829-836 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: "Direct Electron Transfer of Ferredoxin at an Indium Oxide Electrode in the presence of Poly-L-lysine" Denki Kagaku (Journal of Electrochemical Society, Japan). 61. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: "Electrochemistry of myoglobin and cyanomyoglobin from different origins" Journal of Electroanalytical Chemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: "Direct electron transfer of ferredoxin at various electrode in the presence of polycations" Bioelectrochemistry and Bioenergetics.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: Kagaku Kogyo Sha (Chemical Industry Co. Ltd.). Preparation and Application of Functional Modified Electrodes in "Kagaku Kogyo Sha (Chemical Industry) " (in Japanese), 279-285 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI, Isao: The Electrochemical Society, Inc.Direct Electron Transfer Reactions of Proteins at Functional Electrodes in "Proc. 5th Int. Symp. on Redox Mechanisms & Interfacial Properties of Molecules of Biological Importance", 12-500 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] HAWKRIDGE, Fred M.: The Electrochemical Society, Inc.Myoglobin Electron Transfer and Ligand Binding Reactions in "Proc. 5th Int. Symp. on Redox Mechanisms & Interfacial Properties of Molecules of Biological Importance", 12-500

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TANIGUCHI,Isao: "Preparation of a Second generation Glucose Sensor Using a Platinized carbon Paper (PCP) Electrode" Proc.Korea-Japan Joint Symposium on Chemical Sensors. 1. 31-36 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] TANIGUCHI,Isao: "Application of Promoter Modified electrodes to Protein Electrochemistry" Proc.Fifth International Symposium on Electrochemistry. 1. 107-114 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 冨永 昌人: "カ-ボン電極上でのペルオキダ-ゼの電子移動反応" 分析化学. 40. 859-861 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] TANIGUCHI,Isao: "Electrochemistry of Myoglobin and Ferredoxin at an Indium Oxide Electrode" Rev.of Polarography. 37. 12 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] TANIGUCHI,Isao: "Electrocatalytic Reduction of Greenhouse Gases Using Biofunctional Metal Complexes" Proc.of International Symposium on Chemical Fixation of Carbon Dioxide. 1. 81-88 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] TANIGUCHI,Isao: "Direct Electron Transfer of Horse Heart Myoglobin at an Indium Oxide Electrode" J.Electroanal.Chem.

    • Related Report
      1991 Annual Research Report
  • [Publications] 谷口 功: "実験化学講座(日本化学会編) 第9巻 6章「有機電気化学」(pp.381ー392) 執筆" 丸善, 12 592 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 谷口 功: "化学工業(特集号:高機能化する電極とその応用) 「分子修飾による電極の機能制御」 執筆" 化学工業社, 7 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] X.Yuan: "Rate of Conformational Change of Cytochrome c During Electron Transfer Determined by Double Potential Step Chronoelliptometry" J.Am.Chem.Soc.112. 5380-5381 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Tominaga: "Electrochemical Behavior of Ferredoxin and Cytochrome c in the Presence of Mg^<2+> at Various Electrode Surfaces" Rev.of Polarography. 36. 40 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] I.Taniguchi: "Preparation of an inーsitu fluoroelectrochemical cell and its application to biological molecules" J.Electroanal.Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] I.Taniguchi: "Application of Promoter Modified Electrodes to Cytochrome c Electrochemistry" Denki Kagaku.

    • Related Report
      1990 Annual Research Report
  • [Publications] I.Taniguchi: "Direct electron transfer of spinach ferredoxin at various electrodes in the presence of polycations" J.Electroanal.Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] 冨永 昌人: "カ-ボン電極上でのペルオキシダ-ゼの直接電子移動反応" 分析化学.

    • Related Report
      1990 Annual Research Report
  • [Publications] 谷口 功: "現代の電気化学 第8章:バイオエレクトロケミストリ-(199ー221)第10章:情報変換と電気化学 執筆(241ー269)" 新星社, 50 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 谷口 功: "バイオコンピュ-ティング研究戦略 第3章:電子機能タンパク質(81ー86) 執筆" サイエンスフォ-ラム社, 6 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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