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Cooperative studies of seroepidemiology and pathogenesis of Viral and parasitie diseases in Okinawa and Thailand

Research Project

Project/Area Number 02045036
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionUniversity of the Ryukyus

Principal Investigator

FUKUNAGA Toshihiko  Department of Virology, Faculty of Medicine, 医科部, 教授 (10029796)

Co-Investigator(Kenkyū-buntansha) NIWAT MANEEK  チェンナイ大学, 医学部, 准教授
CHIRASAK KHA  チェンマイ大学, 生命科学研究所, 所長
MAKINO Yoshihiro  Department of Virology, Faculty of Medicine, 医学部, 助教授 (60039930)
SATO Yoshiya  Depratment of Parasitology, Faculty of Medicine, 医学部, 教授 (60092699)
IWAMASA Teruo  Department of Pathology II, Faculty of Medicine, 医学部, 教授 (10110842)
KHAMBOONRUANG Chirasak  Research Institute for Health Sciences, Chiang Mai University
MANEEKARN Niwat  Department of microbiology, Faculty of Medicine, Chiang Mai University
NIWAT Maneek  チェンマイ大学, 医学部, 准教授
CHIRASAK Kha  チェンマイ大学, 医学部, 教授
SITHISOMBUT ノポーン  チェンマイ大学, 医学部, 講師
MANEEKARN Ni  チェンマイ大学, 医学部, 准教授
CHIOWANICHI ピエン  チェンマイ大学, 医学部, 教授
大鶴 正満  琉球大学, 医学部, 客員教授 (60018281)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsJapanese encephailtis / Dengue hemerrhgic fever / Cross immune response in flavivirus infections / RT-PCR / Expressed protein by baculovirus system / Heres simplex virus type 2 / Strongyloidiasis / Serodiagnosis of strongyloidiasis / 糞線虫症の血清学的診断法 / 単純ヘルペスウイルスII型 / 単純ヘルペスウイルスII型の病原性 / 日本脳炎(JE) / デング出血熱(DHF) / 単純ヘルペスウイルスII型(HSVーII) / 分子疫学 / 診断用発現蛋白
Research Abstract

The cooperative study consists of three fields : 1. Flavivirus infections, specifically Japanese encephalitis(JE) and dengue hemorrhagic fever(DHF). 2. Herpes simplex virus type 2(HSV 2) infection. 3. Strongyloidiasis.
Results obtained are described briefly as follows :
1. Cross immune response between JE and DHF was examined by neutralization(N) tests. When individuals with pre-existing N antibody to JE or dengue virus were infected sequentially with dengue or JE virus, significant increases of N titer were observed not only to the causative virus but also to the previously infected virus at the frequency of 35%-40%. Thus, even the N test could not always identify the causative virus in re-infection of flaviviruses.
The truncated dengue-4 E protein produced by a recombinant baculovirus was wxamined for the possibility of diagnostic antigen to differentiate dengue and JE infection. Results indicated that the expressed protein was a useful diagnostic antigen in differentiation of dengue in … More fection from JE with slightly lower specificity than N test.
RT-PCR method was examined for the early diagnosis of DHF. Appropriate selections of primers for RT-PCR resulted in a useful identification method of 4 serotypes of dengue virus in comparison with virus isolation.
2. HSV 2 isolates in Okinawa and Chiang Mai were analyzed by the restriction endonuclease cleavage patterns and virulence to mice. Some cleavage patterns characteristic to each regional isolate were observed. The Okinawan isolates showed higher, intermediate and lower virulence while the Chiand Mai isolates showed only intermediate virulence to mice by peripheral injection
3. Field study on strongyloidiasis in Chiang Mai gave positive rates of 46.3% in adult villagers and 6.9% in school children.
The newly developed ELISA for strongyloidiasis gave well-corresponding results with those of the stool culture examination for the Okinawan samples but did not for the Chiang Mai samples, possibly due to cross reactions caused by co-infections with other intestinal parasites such as Opisthorchis viverrini and hookworm, which were found to be highly prevalent in the Chiang Mai area. Less

Report

(3 results)
  • 1992 Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Y.MAKINO et al.: "Potential use of baculobirus-expressed dengue-4 E protein as a diagnotic antigen in regions endemic for dengue and Japnese encephalitis" American Journal of Tropical Medicine and Hygiene. 45. 636-646 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] YOSHITAKE,H.et al.: "Seroepidemiology og herpes simplex and ristriction endonuclease cleavage analysis of simplex virus type 2 in Okinawa" Acta pathol.Jpn.41. 24-30 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SATO,Y.et al.: "Application of enzyme-limked immunosorbent assay for mass examinaion of strongyloidiasis in Okinawa,Japan" International Journal for Parasitology. 20(8). 1025-1029 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SATO,Y.ea al.: "Gelatine particle inditect agglutination test for mass examination for strongyloidiasis" Transaction of the Royal Society of Tropical Medicine and Hygiene. 85(4). 515-518 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y. Makino et al.: "Potential use of a baculovirus-expressed dengue-4 E protein as a diagnostic antigen in regions endemic for dengue and Japanese encephalitis" American Journal of Tropical Medicine and Hygiene. 45. 636-646 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshitake, H. et al.: "Seroepidemiology of herpes simplex virus and restriction endonuclease cleavage analysis of herpes simplex virus type 2 in Okinawa" Acta Pathol. Jnp.41. 24-30 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato, Y. et al.: "Application of enzyme-linked immunosorbent assay for mass examination for strongyloidiasis in Okinawa, Japan" International Journal for Parasitology. 20(8). 1025-1029 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato, Y. et al.: "Gelatine particle indirect agglutination test for mass examination for strongyloidiasis" Transaction of the Royal Society of Tropical Medicine and Hygiene. 85(4). 515-518 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Makino,M.Tadano,S.Arakaki and T.FUKUNAGA: "Potential use of a baculovirus-Expressed denue-4 E protein as a diagnostic ant igen in regions endemic for dengue and Japanese encephalitis" American Journal of Tropical medicine and Hygiene. 45. 636-646 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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