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Studies on dynamic changes of molecular distribution in plant cells by immuno-electron microscopy

Research Project

Project/Area Number 02304007
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物形態・分類学
Research InstitutionThe University of Tokyo

Principal Investigator

KUROIWA Tsuneyoshi  The Univ. of Tokyo, Facl. Sci., Professor, 理学部, 教授 (50033353)

Co-Investigator(Kenkyū-buntansha) UEDA Katsumi  The Nara Women's Univ., Facl. Sci., Professor, 理学部, 教授 (00031641)
TANAKA Kenji  The Nagoya Univ., Medical College, Professor, 医学部, 教授 (70013315)
MURAKAMI Satoru  The Univ. of Tokyo, College of Arts and Sciences, Professor, 教養学部, 教授 (70012367)
OSAFUNE Tetsuaki  The Tokyo Medical College, Lecture, 医学部, 講師 (70074630)
HORI Terumitsu  The Univ. of Tsukuba, Facl. Sci., Professor, 生物科学系, 教授 (90057563)
山口 正視  東京慈恵会, 医学部, 講師 (90147364)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥20,700,000 (Direct Cost: ¥20,700,000)
Fiscal Year 1992: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1991: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1990: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsimmuno-electron microscopy / molecular distribution / coloidal gold / epifouorescence microscopy / green algae / plant cells / yeast / organelles / 免疫電子顕微鏡法 / 抗DNA抗体 / ミトコンドリア / 葉緑体DNA / ライソゾ-ム / アビジン・ゴ-ルド標識 / 植物形態 / 免疫光学・電子顕微鏡法 / ピレノイド / ゴルジ体
Research Abstract

We have developed an immuno-light-electron microscopy and examined the dynamic changes of DNA and proteins in plant cells. As a result, we have obtained new data about these problems. Especially, Osafune and Ueda improved the immuno-gold-electron microscopy (IGEM) to obtain a high resolution. The newly developed techniques are applied to various fields of these investigators including the group. Kuroiwa developed a technique for the simultaneous observations of light microscopic field and electron microscopic field and applied the technique to analyzing the multiplication of cells in root meristems of Pelargonium zonale, Nicotiana tabacum and Arabidopsis thaliana. The results showed that the mtDNA content per mt-nucleus in the cells just above quiescent center, where the synthesis of mtDNA is active, corresponds to approximately 3000 kilobase pairs but, in the meristematic cells just below the elongation zone of the root it falls less than 170 kbp. Murakami showed using IGEM that the photosystem I is located in thylakoid membrane systems, while the photosystem II in stroma thylakoids. Osafune found by a high resolution IGEM that LHCPII apoprotein transferred to plastid through the Golgi apparatus. Ueda showed that the actin is located on the surface of lysosomes in Trebouxia. Tanaka, Yamaguchi and Miyakawa revealed the behavior of proteinase, the formation of virus-like particles and the existence of mitchondorial nuclear proteins in yeast cells, respectively.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Suzuki,T.and Kuroiwa,t.: "Prefercutial wn'tochondrial and plastid DNA synthesis before multiple cell divisions in Nicotiana tabacum" J.Cell Sci.103. 831-837 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato,N.and Kuroiwa,T.: "Detection and characterization of a plartid envelope DNA-binding protein whicmay anchor nucleoids" The EMBO Journal. 12. 555-561 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Osafune,t.: "Accumulation of LGCP II apoprotein in wax-richcells of Euglena in Iight or in the presence of streptomycin" J.Struct.Bial.109. 97-108 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Cheda,Y.and Ueda: "Detection of actin on oraganlles of Trebouxia pottei,in particular on The surface of lysosoned" Protoplasma. 171. 28-33 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Murakami,S.: "Structural and bunctional argnization of the thylarkoidmembrane system in photosynthetic apparatus" J.Electyon Mecros.41. 424-433 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Homma,M.and Tanaka,K.: "Detection of intracellular forms of secretory aspartic proteinase in Candida albicans" J.Genoral Microbiol.138. 627-633 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Suzuki,T. and Kuroiwa,T.: "Preferential mitochondrial and plastid DNA synthesis before multiple cell divisions in Nicotiana tabacum" J.Cell Sci.103. 831-837 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato,N. and Kuroiwa,T.: "Detection and characterization of a plastid envelope DNA binding protein which may anchor nucleoids" The EMBO Journal. 12. 555-561 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Osafune,T.: "Accumulation of LHCII apoprotein in wax-rich cells of Euglena in light or in the presence of streptomycin" J.Struct.Biol.109. 97-108 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chida,Y. and Ueda,K.: "Detection of actin on organelles of Trebouxia pottei in particular on the surface of lysosomes" Protoplasma. 171. 28-33 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Murakami,S.: "Structural and functional organization of the thylakoid membrane system in photosynthetic apparatus" J.Elec.Micro.41. 424-433 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Homma,M. and Tanaka,K.: "Detection of intracellular forms of secretory aspartic proteinase in Candida albicans" J.General Microbiol.138. 627-633 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Suzuki,T and Kuroiwa,T.: "Preferential mitochondrial and Plastid DNA synthesis before multiple cell divisions in Nicotiana tabacum" J.cell Sci.103. 831-837 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sato,N.and Kuroiwa,T.: "Detection and characterization of a plastid envelope DNA-binding protein whichmay anchor nucleoids" The EMBO Journal. 12. 555-561 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Osafune,T.: "Accumulation of LHCP II apoprotein in Wax-rich cells of Euglena in bight or in the presence of streptomycin" J.Struct.bial.109. 97-108 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Chida,Y.and ueda: "Detection of actin on organelles of Trebouxia pottei,in particular on The surface of lysosomes" Protoplasma. 171. 28-33 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Murakami,S.: "Structural and functional organization of the thy lakoidmembrane system in photosynthetic apparatus" J.General Microbiol.138. 627-633 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kuroiwa,T.: "The replication,differentiation,and inheritance of plastids with emphasis on the concept of organelle nuclei" International Review of Cytolgy. 128. 1-62 (1961)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hori,T.and Green,J.C.: "The ultrastructure of the flagellar root system of Isochrysis galbana Parke(Prymnesiophyta)." J.Mar.Biol.Asso.U.K.71. 137-152 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Chida,Y.and Ueda,K.: "Division of chloroplasts in a green alga,Trebouzia potteri." Ann.Bot.67. 435-442 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Osafune,T.,Schiff,J.F.and Hase,E.: "Stage‐dependent localization of LHCP II apoprotein in the Golgi of synchronized cells of Euglena gracilis by immunogold electronmicroscopy" Exp.Cell Res.193. 320-330 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nemoto,Y.,Kawano,S.,Nagata,T.and Kuroiwa,T.: "Studies on plastid‐nuclei(nucleoids)in Nicotiana tabacum IL.IV.Association of chloroplast DNA with proteins at several specific sites in isolated chloroplast nuclei" Plant Cell Physiology. 131-141 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sando,N.,Miyakawa,I.,Miyamoto,M.,Araki,N.and Kuroiwa,T.: "Configurational changes of mitochondrial nucleoids during the growth of the yeasts,Saccharomyces cerevisiae,uinder aerobic or anaerobic conditions." J.Cell Science.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamaguchi,M.,Sugahara,K.and Mizokami,H.: "Biotechnology Current Progress" Technomic Publishing Co.,Inc.,14 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kuroiwa,T.,Kuroiwa,H.,Mita,T.& M.Fujie: "Fluorescence microscopic study of the formotion of giant mitochondrial nuclci in the young ovules of <Pelargoniam>___ー <zonale>___ー." Protoplasma. 158. 191-194 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuroiwa,T.,Fuje,M.,Mita,T.& H.Kuroiwa: "Application of embedding of samples in Technovit 7100 regin to observations of small amounts of DNA in the cellular organelles associated with cytoplacmic inheritance" Fluores.Tech.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kanbe,T.,Hiraoka,Y.,Tanaka,K.& Yanagida,M.: "The transition of cells of bission yerst βーtubulin mutant <nda3ー311>___ー as seen by freezeーsubstitution electron microscopy" J.Cell Sci.96. 275-282 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ueda,K.& Y.Goto: "The anaphase movement of chromosomes in <Tradescantia>___ー <reflexa>___ー and <Spirogyra>___ー" Cytologia. 55. 265-271 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inouye,I.,Hori,T.& M.Chihara: "Absolute configuration analysis of the flagellar apparatus of <Pteruspermo>___ー <cristatum>___ー(Prasinophyceae)and consideration of its phylogenetic position" J.Phycology. 26. 329-344 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kobayashi,H.,Kitabayashi,K.,Matsumoto,K.& Hirokawa,H.: "Primer Protein of bacteriophage M2 exposes the RGD receptorsite upon linking the first deoxynucleotide" Mol.Gen.Genet.(1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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