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MOLECULAR PATHOLOGY OF PLANT INFECTION MEDIATED BY HOST-SPECIFIC TOXINS

Research Project

Project/Area Number 02304019
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionTOTTORI UNIVERSITY

Principal Investigator

KOHMOTO Keisuke  TOTTORI UNIV., FAC. OF AGRIC., PROFESSOR, 農学部, 教授 (80032093)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hirokazu  SHIZUOKA PREFEC.UNIV., FAC.OF FOOD AND NUTRITION SCIENCE, ASSIST.PROF., 食品栄養科学部, 助教授 (80170348)
TSUGE Takshi  NAGOYA UNIV., FAC. OF AGRIC., ASSIST.PROF., 農学部, 助教授 (30192644)
NAKATSUKA Shinichi  GIFU UNIV., FAC. OF AGRIC., ASSIST.PROF., 農学部, 助教授 (30109318)
ARASE Sakae  SHIMANE UNIV., FAC. OF AGRIC., ASSIST.PROF., 農学部, 助教授 (40127478)
OTANI Hiroshi  TOTTORI UNIV., FAC. OF AGRIC., PROFESSOR, 農学部, 教授 (50032305)
中島 広光  鳥取大学, 農学部, 助教授 (40144646)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥25,700,000 (Direct Cost: ¥25,700,000)
Fiscal Year 1992: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1991: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1990: ¥10,800,000 (Direct Cost: ¥10,800,000)
KeywordsHOST-SPECIFIC TOXIN / HOST-SELECTIVE TOXIN / ALTERNARIA / GENE FOR PATHOGENICITY / BIOSYNTHETIC PATHWAY OF FUNGAL TOXIN / TOXIN RECEPTOR / ULTRASTRUCTURAL MODIFICATION OF CELL MEMBRANE / INFECTION-INDUCING EFFECT / 環状デプシペプチド / 毒素レセプタ- / 宿主識別因子 / 受容性誘導 / 形質転換ベクタ- / 原形質膜 / アルタ-ナリア
Research Abstract

An interdisiplinary project for 3 years was conducted to elucidate the molecular basis of plant infection by toxigenic fungal pathogens with host-specific toxins (HST). A proposed scheme for disease establishment that an HST released during spore germination binds a putative toxin-binding site (receptor) in potential host plant, where the HST-signal induces the state of accessibility to the invader, and then the producing fungus is eventually able to colonize the plant, was developed as follows:
1) ACT-, BZR-, AT- and Pyricularia oryzae-toxins were isolated from germination fluids of the pathogens, and structures of the former two toxins were elucidated. ACT-toxin shared in structure a decatrtienoic acid with AK- and AF-toxins, while BZR-toxin composed of homologs of cyclic depsipeptides that were bioactive only when they co-exisited at least two members (co-toxin). On the other hand, a structure-activity corelation study revealed that there is coincidence between the host ranges of the … More Japanese pear, strawberry and tangerine pathotypes of Alternaria alternata and the plant ranges sensitive to AK-, AF- and ACT-toxins they produced, suggesting that host-specificity of the fungus is precisely determined at the molecular level.
2) Biosynthetic pathways of AK-, AF- and ACT-toxins were proposed, depending on structural analysis of intermediate metabolites and tracer experiments. Enzymatic basis for AM-toxin synthesis also examined.
3) Cascade-like signal transduction processes of HSTs actions were dissected by means of physiological, biochemical and ultrastructural approaches.
4) A highly efficient integrative transformation system was estalished for Alternaria alternata: the vector pDH25rla was constructed by inserting a highly repetitive ribosomal RNA gene cluster (rDNA) sequence into a unique Xbal site of pDH25. Using this, 3 genes for fungal melanin biosynthesis (ALM, BRM1, BRM2) were isolated as a model for cloning pathogenicity genes.
5) For HST-receptor studies, anti-HST anibodies were generated. A possible receptor protein was detected in the plasma membrane fraction of susceptible pear calli but not of resistant cell line derived from the same susceptible calli. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (134 results)

All Other

All Publications (134 results)

  • [Publications] Shimomura,N.,et al.: "Two action sites of AM-toxin produced by Alternaria alternata apple pathotype and their pathological significance" Ann.Phytopathol.Soc.Japan. 57. 247255 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kodama,m.,et al.: "Effect of AL-toxin produced by Alternaria alternata tomato bathotype on viability of cultured tomato cells determined by MTT-colorimetric assay" Ann.Phytopathol.Soc.Japan. 57. 663-670 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Feng,B.-N.,et al.: "Biosynthesis of host-selective toxins produced by Alternaria alternata pathogens.I.(8R,9S)-9,10-epoxy 8-hydroxy-9-methyl-deca(2E,4Z,6E)-trienoic acid,a precursor of AK-toxins" Agric.Biol.Chem.54. 845-848 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakatsuka,S.,et al.: "Biosynthetic origin of (8R,9S)-9,10-epoxy-8-hydroxy-9-methyl-deca-(2E,4Z,6E)-trienoic acid,a percursor of AK-toxins produced by Alternaria alternata Japanese pear pathotype" Phytochemistry. 29. 1529-1531 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakajima,H.,et al.: "Spiciferones B and C,minor phytotoxins from Cochliobolus spicifer" Phytochemistry. 30. 2563-2565 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao,J.-Z.,et al.: "Isolation and characterization of a factor from Bipolaris zeicola race 3 that induces susceptibility in rice plants" Ann.Phytopath.Soc.Japan. 56. 605-612 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsuge,t.,et al.: "Efficient integrative transformation of the phytopathogenic fungus Alternaria alternata mediated by the repetitve rDNA sequences" Gene. 92. 207-214 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] HAYASHI,N.etal.: "Determination of host-selective toxin production during spore germina-tion of Alternaria alternata by high perfomance liquid chromatography" Phyropathology. 80. 1088-1091 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kobayashi,H.,et al.: "Transcriptional regulation and DAN methylation in plastids during transitional conversion of chloroplasts to chromoplasts" EMBO Journal. 9. 307-313 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao,J.-Z.,et al.: "Rice-specific toxins produced by Bipolaris zeicola race 3:Evidence for role as pathogenicity factors for rice and maize plants" Physiol.Molec.Plant Pathol.38. 67-82 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee,S.-S.,et al.: "Effext of host-specific Af-toxins from Alternaria alternata strawberry pathotype on viability of strawberry cultured cells" Ann.Phytopath.Soc.Japan. 57. 671-679 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto,K.,et al.: "Host recognition by toxigenic plant pathogens" Experientia. 47. 755-764 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 尾谷 浩 他: "宿主特異的毒素についての最近の研究成果" 植物防疫. 44. 450-454 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 甲元 啓介: "宿主特異的毒素と病害防除戦略" 日本植物病理学会感染生理談話会論文集. 26. 37-46 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Arase,S.,et al.: "Induction of inaccessibility to Pyricularia oryzae by pre-inoculation of P.grisea in rice leaf-sheath cells" Jour.Phytopathology. 134. 97-102 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakajima,H.,et al.: "Biosynthesis of spiciferinin, a unique metabolite of the phytopathogenic fungus,Cochliobolus spicifer" J.Chem.Soc.,Chem.Commun.1992. 1654-1656 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee,S.-S.et al.: "Protective effect of homologues of host-specific AF-toxin I produced by Alternaria alternata strawberry pathotype on strawberry cells" Jour.Phytopathology. 135. 177-186 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Park,P.,et al.: "Temporary activation of cellular metabolism in susceptible Japanese pear leaves responding to AK-toxin(Uitrastructural morphometric studies)" Ann.Phytopath.Soc.Japan. 58. 234-243 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto,K.,et al.: "Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to host-specific toxins" Phytopathology. 81. 719-722 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimomura,N.,et al.: "Effects of light and SH-reagent on ultrastructural changes in leaf cells induced by AM-toxin from Alternaria alternata apple pathotype" Ann.Phytopath.Soc.Japan. 58. 305-309 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza,N.P.,et al.: "Effect of AL-toxin produced by Alternaria altrenata tomato pathotype on cultured roots of tomato" Ann.Phytopath.Soc.Japan. 58. 411-415 (1192)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Park P.,et al.: "Slightly damaged type of plasma membrane modification in strawberry leaves treated with AF-toxin I produced by Alternaria alternata strawberry pathotype" Ann.Phytopath.Soc.Japan. 58. 431-445 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakajima,H.,et al.: "Spiciferinone,an azaphilone phytotoxin produced by the fungus Cochliobolus spicifer" Phytochemistry. 32. 105-107 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao,J.-Z.,et al.: "Further evaluation of the significance of BZR-toxin produced by Bipolaris zeicola race 3 in pathogenesis on rice and maize plants" Physiol.Molec.Plant Pathol.40. 359-370 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柘植 尚志 他: "植物病原糸状菌のRFLP" 植物防疫. 46. 315-319 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 尾谷 浩 他: "病原菌の宿主特異性とその分子機構:宿主特異珍と宿主特異的毒素" 植物細胞工学. 5. 5-11 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nutsugah,S.K.,et al.: "Production of a host-specific toxin by germinating spores of Alternaria tenuissima causing leaf spot of pigeon pea" Jour.Phytopathology. 136. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nutsugah,S.K.,et al.: "Iltrastructural changes in pigeon pea cells caused by a host-specific toxin from Alternaria tenuissima" Ann.Phytopath.Soc.Japan. 59. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto,K.,et al.: "Occurrence of the Japanese pear pathotype of Alternaria alternata in pear orchards in Italy" Phytopathologia Mediterranea. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 甲元 啓介: "植物病原糸状菌による宿主識別:宿主特異的毒素の介在とその分子機構" 学術月報. 46. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Arase,S.,et al.: "Effects of thermal and cycloheximide per-treatments on light-dependent necrosis formation to rice leaves by Pyricuraria oryzae toxin(s)" Jour.Phytopathol.135. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Itoh,Y.,et al.: "A toxin from the tangerine pathotype of Alternaria alternata that has selective toxicity to Japanese pear" Ann.Phytopath.Soc.Japan. 59. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto,K.,et al.: "Isolation and biological activities of two host-specific toxins from the tangerine pathotype of Alternaria alternata" Phytopathology. 83. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 中島 路可 他: "長鎖α-およびβ-アミノ酸の花粉管の伸長阻害効果" Chemistry Express. 7. 481-484 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 上田 和生 他: "トウモロコシの病原菌B.zeicola race3が生産する宿主特異的BZR毒素群の構造" 第33回天然有機化合物討論会講演要旨集(大阪). 33. 659-666 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 奥 八郎,甲元 啓介 他(編): "植物感染生理学最近の進歩" 植物感染生理学最近の進歩刊行会(名古屋大学), 250 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Patill,S.S.,et al.(Eds.): "Molecular Srtategies of Pathogens and Host Plants" Springr-Verlag,Berlin & New York, 250 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] J.Chelkowski et al.(Ede.): "Alternaria-Metabolites,Biology and Plant Diseases" Elsevier Sci.Publ., 573 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 久能 均(編): "生物相互間の認識機構" 日本植物病理学会, 145 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.F.Linskens et al.: "Modern Methods of Plant Analysis (N.S.)vol.13,Plant Toxin Analysis" Springer-Verlag, 389 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakatsuka, S., et al.: "Biosynthetic origin of (8R,9S)-9,10-epoxy-8-hydroxy-9-methy-deca-(2E,4Z,6E)-trienoic acid, a precursor of AK-toxins produced by Alternaria alternata Japanese pear pathotype" Phytochemistry. 29. 2563-2565 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Feng, B.-N., et al.: "Biosynthesis of host-selective toxins produced by Alternaria alternata pathogens. I.(8R,9S)-9,10-epoxy-8-hydroxy-9-methyl-deca-(2E,4Z,6E)-trienoic acid, a precursor of AK-toxins" Agric.Biol. Chem.54. 845-848 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao,J.-Z., et al.: "Isolation and characterization of a factor from Bipolaris zeicola race 3 that induces susceptibility in rice plants" Ann.Phytopath.Soc. Japan. 56. 605-612 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsuge, T., et al.: "Efficient integrative transformation of the phytopathogenic fungus Alternaria alternata mediated by the repetitive rDNA sequences" Gene. 92. 207-214 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hayashi, N., et al.: "Determination of host-selective toxin production during spore germination of Alternaria alternata by high performance liquid chromatography" Phytopathology. 80. 1088-1091 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Otani, H., et al.: "Recent advances in research of host-specific toxins (in Japanese)" Shokubutsu-Boueki. 44. 450-454 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto, K.: "Disease control strategies from the viewpoint of host-specific toxins (in Japanese)" Proc. 26th Symp.on Physiol.Plant Pathol.37-46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimomura, N., et al.: "Two action sites of AM-toxin produced by Alternaria alternata apple pathotype and their pathological significance" Ann.Phytopath.Soc. Japan. 57. 247-255 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kodama, M., et al.: "Effect of AL-toxin produced by Alternaria alternata tomato pathotype on viability of cultured tomato cells determined by MTT-colorimetric assay" Ann.Phytopath.Soc. Japan. 57. 663-670 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee, S.-S., et al.: "Effect of host-specific AF-toxins from Alternaria alternata strawberry pathotype on viability of strawberry cultured cells" Ann.Phytopath.Soc. Japan. 57. 671-679 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao, J.-Z., et al.: "Rice specific toxins produced by Bipolaris zeicola race 3: Evidence for role as pathogenicity factors for rice and maize plants" Physiol.Molec. Plant Pathol.38. 67-82 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto, K., et al.: "Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to host-specific toxins" Phytopathology. 81. 719-722 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto, K., et al.: "Host recognition by toxigenic plant pathogens" Experientia. 47. 755-764 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakajima, H., et al.: "Spiciferones B and C, minor phytotoxins from Cochliobolus spicifer" Phytochemistry. 30. 2563-2565 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ueda, K., et al.: "Structures of host-selective BZR-toxins produced by B. zeicola race 3 that causes disease in corn" 33rd Symp.Papers Chem.Natural Pro- ducts. 659-666 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Arase, S., et al.: "Induction of inaccessibility to Pyricularia oryzae by pre-inoculation of P. grisea in rice leaf-sheath cells" J. Phytopathol. 134. 97-102 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee, S.-S., et al.: "Protective effect of homologues of host-specific AF-toxin I produced by Alternaria alternata strawberry pathotype on strawberry cells" J. Phytopathol. 135. 177-186 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakajima, H., et al.: "Biosynthesis of spiciferinin, a unique metabolite of the phytopathogenic fungus, Cochliobolus spicifer" J.Chem.Soc.,Chem. Commun.1654-1656 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Park, P., et al.: "Temporary activation of cellular metabolism in susceptible Japanese pear leaves responding to AK-toxin (Ultrastructural morphometric studies)" Ann.Phytopath.Soc. Japan. 58. 234-243 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsuge, T., et al.: "RFLP analysis in phytopathogenic fungi (in Japanese)" Shokubutsu-Boueki. 46. 315-319 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimomura, N., et al.: "Effects of light and SH-reagent on ultrastructural changes in leaf cells induced by AM-toxin from Alternaria alternata apple pathotype" Ann.Phytopath.Soc. Japan. 58. 305-309 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza, N., et al.: "Effect of AL-toxin produced by Alternaria alternata tomato pathotype on cultured roots of tomato" Ann.Phytopath.Soc. Japan. 58. 411-415 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Park, P., et al.: "Slightly damaged type of plasma membrane modification in strawberry leaves treated with AF-toxin I produced by Alternaria alternata strawberry pathotype" Ann.Phytopath.Soc. Japan. 58. 431-445 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakajima, H., et al.: "Spiciferinone, an azaphilone phytotoxin produced by Cochliobolus spicifer" Phytochemistry. 31. 105-107 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao, J.-Z., et al.: "Further evaluation of the significance of BZR-toxin produced by Bipolaris zeicola race 3 in pathogenesis on rice and maize plants" Physiol. Molec. Plant Path.40. 359-370 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nutsugah, S.K., et al.: "Production of a host-specific toxin by germinating spores of Alternaria tenuissima causing leaf spot of pigeon pea" J.Phytopathol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nutsugah, S.K., et al.: "Ultrastructural changes in pigeon pea cells caused by a host-specific toxin from Alternaria tenuissima" Ann.Phytopath.Soc. Japan. 58.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto, K., et al.: "Occurrence of the Japanese pear pathotype of Alternaria alternata in pear orchards in Italy" Phytopath. Medit.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Arase, S., et al.: "Effects of thermal and cycloheximide pre-treatments on light-dependent necrosis formation to rice leaves by Pyricularia oryzae toxin(s)" J.Phytopathol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Itoh, Y., et al.: "A toxin from the tangerine pathotype of Alternaria alternata that has selective toxicity to Japanese pear" Ann.Phytopath.Soc. Japan. 58.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto, K., et al.: "Isolation and biological activities of two host-specific toxins from the tangerine pathotype of Alternaria alternata" Phytopathology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kohmoto, K.: "Molecular biology of host-specific toxin-mediated host recognition by plant fungal pathogens (in Japanese)" Gakujutsu-Geppo.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Oku, H., et al.(Eds.): The Publishing Committee, Nagoya. Recent Advances in Physiological Plant Pathology (in Japanese), 233 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Patill, S.-S., et al.(Eds.): Springer-Verlag, Berlin and New York. Molecular Strategies of Pathogens and Host Plants, 250 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kunoh, H.(Ed.): Phytopathological Society of Japan, Tokyo. The Dynamics of Recognition in Interactions of Organisms (in Japanese), 145 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chelkowski, J., et al.(Eds.): Elsevier Sci.Public.Alternaria: Metabolites, Biology and Plant Diseases, 573 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Linskens, H.F., et al.(Eds.): Springer-Verlag, Berlin. Modern Methods of Plant Analysis (N.S.) vol.13, Plant Toxin Analysis, 389 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Arase,S.,et al.: "Induction of inaccessibility to Pyricularia oryzae by pre-inoculation of P.grisea in rice leaf-sheath cells" J.Phytopathology. 134. 97-102 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Arase,S.,et al.: "Effects of thermal and cycloheximide pre-treatments on light-dependent necrosis formation to rice leaves by Pyricuraria oryzae toxin(s)" J,Pgytopathology. 135. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] ITOH,Y.,et al.: "A toxin from the tangerine pathotype of Alternaria alternata that has selective toxivety to Japanese pear" Ann.Phytopathol.Soc.Japan. 59. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kohmoto,K.,et al.: "Isolation and biological activities of two host-specific toxins from the tangerine pathotype of Alteinaria alternata" Phytopathology. 83. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 甲元啓介: "植物病原糸状菌による宿主識別に宿主特異的毒素の介在とその分子機構" 学術月報. 46. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakajima,H.,et al.: "Biosynghesis of spicifernin,a unique metabolieta of the phytopathogenic fungus,Cochliobolus rpicifer" J.Chim.SoC.,Chem.Commun.,. 1992. 1654-1656 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Lee,S.-S.,et al.: "Protective effect of hoologues of host-specific AF-toxin I produced by Alternaria alternata strawberry paghotype on strawberry cells" J.Phytopathol.135. 177-186 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nutsugah,S.K.,et al.: "Production of a host-specific toxin by germinating spores of Alternaria tinuissima causing leaf spot of pigeon pea" J.Phytopathol.136. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nutsugah,S.K.,et al.: "Ultrastructural changes in pigeon pea cells caused by a host-specific toxin from Alternaria temuissima" Ann.Phytopathol.Soc.Japan. 59. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Orolaza,N.P.,et al.: "Effect of AL-toxin produced ny Alternaria alternata tomato paghotype on cultured roots of tomato" Ann.Phytopathol.Soc.Japan. 58. 411-415 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 尾谷 浩 他: "病原菌の宿主特異性とその分子機構:宿主特異性と宿主特異的毒素" 植物細胞工学. 5. 5-11 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Park,P.,et al.: "Slightly damaged type of plasma membrane in strawberry leaves treated with AF-toxin I produced by Alternaria alternata straw-berry pathotype" ANN.Phytopathol.Soc.Japan. 58. 431-445 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Park,P.,et al.: "Temporary sctivation of cellular metabolism in susceptible Japanese pear leaves responding to AK-toxin(Ultrastructural morphometric studies)" Ann.Phytopathol.Soc.Japan. 58. 234-243 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimomura,N.,et al.: "Effects of light and SH-reagent on ultrastructural changes in leaf cells induced by AM-toxin from Alternaria altermata apple pathotype" Ann.Phytopathol.Soc.Japan. 58. 315-319 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柘植 尚志 他: "植物病原糸状菌のRFLP" 植物防疫. 46. 315-319 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Xiao,J.,-Z.,et al.: "Further evaluation of the significance of BZR-toxin produced by Bipolaris zeicola race 3 in pathogenesis on rice and maize plants" Physiol.Molec Plant Pathol.40. 359-370 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kohmoto,K.,et al.: "Occurrence of ghe Japanese pear pathotype of Alternaria alternata in pear orchards in Italy" Phytopathologia Mediterranea. 31. 141-147 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Chelkowski et al.(Eds.)(H.Otani & K.Kohmoto,): "Alternaria:Biology,Plant Diseases and Metabolites(Chapter,Host-Specific Toxins Alternaria species)" Elsevier, 573 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.F.Linskens et al.(Eds.)(K.Kohmoto): "Modern Methods of Plant Analysis(N.S.)vol.13 Plant Toxin Analysis(Chapter,Determination of Host-Spec2fic Toxins)" Springer-Verlag, 389 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimomura,N,et al.: "Two action sites of AMーtoxin produced by Alternaria alternata apple pathotype and their pathological significance" Ann.Phytopath.Soc.Japan. 57. 247-255 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kodama,M.,et al.: "Effect of ALーtoxin produced by Alternaria alternata tomato pathotype on viability of cultured tomato cells determined by MTTーcolorimetric assay" Ann.Phytopath.Soc.Japan. 57. 663-670 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kawaguchi,I.,et al.: "Changes in amino acid and amine contents of susceptible tomato infected with Alternaria alternata tomato pathotype and and treated with ALーtoxin" Ann.Phytopath.Soc.Japan. 57. 526-533 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Lee,S.ーS.,et al.: "Effect of hostーspecific AFーtoxins from Alternaria alternata strawberry pathotype on viability of strawberry cultured cells" Ann.Phytopath.Soc.Japan. 57. 671-679 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kohmoto,K.,et al.: "Host recognition by toxigenic plant pathogens" Experientia. 47. 755-764 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kohmoto,K.,et al.: "Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to hostーspecific toxins" Phytopathology. 81. 719-722 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Xiao,J.Z.,et al.: "Riceーspecific toxins produced by Bipolaris zeicola race 3: Evidence for role as pathogenicity factors for rice and maize prants" Physiol.Mol ec.Plant Pathol.38. 67-82 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kobayashi,H.,et al.: "Sequence and expression of genes encoding the large and small subunits of ribulose 1,5ーbisphosphate carboxylase/oxygenase from Chromatium vinosum" Gene. 97. 55-62 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Park,P.,et al.: "Temporary activation of cellular metabolism in susceptible Japanese pear leaves responding with AKーtoxin(Ultrastructural morphometric study)" Ann.Phytopath.Soc.Japan. 58. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shimomura,N.,et al.: "Effects of light and SHーreagent on ultrastructural changes in leaf cells induced by AMーtoxin from Alternata alternata apple pathotype" Ann.Phytopath.Soc.Japan. 58. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nakajima,H.,et al.: "Spiciferinone,an azaphilone phytotoxin produced by the fungus Cochliobolus spicifer" Phytochemistry. 30. 105-107 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Lee,S.ーS.,et al.: "Protective effect of homologues of hostーspecific AFーtoxin I produced by Alternaria alternata strawberry pathotype on strawberry cells" J.Phytopathology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Arase,S.,et al.: "Induction of inaccessibility to Pyricularia oryzae by preーinoculation of P.grisea in rice leafーsheath cells" J.Phytopathology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Arase,S.,et al.: "Effect of thermal and cycloheximide preーtreatments on light dependent necrosis formation to rice leaves by Pyricularia oryzae toxins" J.Phytopathology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久能 均(編) (柘植・分担執筆): "生物相互間の認識機構" 日本植物病理学会, 145 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Chelkowski(ed.) (Otani,H & Kohmoto,K.分担執筆): "AlternariaーMetabolites,Biology and Plant Diseases" Elsevier Sci.Publ.,

    • Related Report
      1991 Annual Research Report
  • [Publications] Shimomura,N.,et al.: "Two action sites of AMーtoxin produced by <Alternaria>___ー <alternata>___ー apple pathotype and their pathological significance" Ann.Phytopath.Soc.Japan. 57. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kodama,M.,et al.: "Effect of ALーtoxin on viability of cultured tomato cells determined by MTTーcolorimetric assay" Ann.Phytopath.Soc.Japan. 57. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 尾谷 浩、他: "宿主特異的毒素についての最近の研究成果" 植物防疫. 44. 450-454 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 甲元 啓介: "宿主特異的毒素と病害防除戦略" 日本植物病理学会感染生理談話会文集. 26. 37-46 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Feng,B.ーN.,et al.: "Biosynthesis of hostーselective toxins produced by <Alternaria>___ー <alternata>___ー pathogens.I.(8R,9S)ー9,10ーepoxyー8ーhydroxyー9ーmethylーdecaー(2E,4Z,6E)ーtrienoic acid,a precursor of AKーtoxins" Agric.Biol.Chem.54. 845-848 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakatsuka,S.,et al.: "Biosynthetic origin of (8R,9S)ー9,10ーepoxyー8ーhydroxyー9ーmethylーdecaー(2E,4Z,6E)ーtrienoic acid,a precursor of AKーtoxins produced by <Alternatria>___ー <alternata>___ー Japanese pear pathotype" Phytochemistry. 29. 1529-1531 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Xiao,J.ーZ.,et al.: "Isolation and characterization of a factor from <Bipolaris>___ー <zeicola>___ー race 3 that induces susceptibility in rice plants" Ann.Phytopath.Soc.Japan. 56. 605-612 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakajima,H.,et al.: "Spiciferones B and C,minor phytotoxins from <Cochliobolus>___ー <spicifer>___ー" Phytochemistry. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Arase,S.,et al.: "Studies on hostーselective infection mechanism of <Pyricularia>___ー <oryzae>___ー Cavara (4) Role of toxin in pathogenesis" Ann.Phytopath.Soc.Japan.

    • Related Report
      1990 Annual Research Report
  • [Publications] Park,P.,et al.: "Improved fixation and embedding methods for electron microscopy of <Alternaria>___ー <alternata>___ー spores" Ann.Phytopath.Soc.Japan. 56. 16-25 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tsuge,T.,et al.: "Efficient integrative transformation of the phytopathogenic fungus <Alternaria>___ー <alternata>___ー mediated by the repetitive rDNA sequences" Gene. 92. 207-214 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hayashi,N.,et al.: "Determination of hostーselective toxin production during spore germination of <Alternaria>___ー <alternata>___ー by high performance liquid chromatography" Phytopathology. 80. 1088-1091 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Xiao,J.ーZ.et al.: "Riceーspecific toxins produced by <Bipolaris>___ー <zeicola>___ー race 3:Evidence for role as pathogenicity factors for rice and maize plants" Physiol.Molec.Plant Pathol.37. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 柘植 尚志: "植物病原糸状菌<Alternaria>___ー <alternata>___ーの宿主特異的毒素." 土と微生物. 37. 15-25 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 中島 路可,他: "長鎖アルキル基を有するαーアミノ酸の合成とその花粉管伸長阻害活性" Chemistry Express.

    • Related Report
      1990 Annual Research Report
  • [Publications] 田平 弘基,他: "ナシのウイルスフリ-化と試験管内増殖" バイオホルティ. 4. 109-113 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 裕和,他: "ハイブリダイゼ-ション:原理と応用" 植物細胞工学. 2. 543-555 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kobayashi,H.,et al.: "Transcriptional regulation and DNA methylation in plastids during transitional conversion of chloroplasts to chromoplasts" EMBO Journal. 9. 307-313 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Patill,S.S.,et al.(ed.): "Molecular Strategies of Pathogens and Host Plants(分担執筆)" SpringerーVerlag,Berlin, 250 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 奥 八郎,他(編): "植物感染生理学最近の進歩" 西村正暘失生追悼記念植物感染生理学最近の進歩刊行会(名古屋大学), 250 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 菊本 敏雄(編)(分担執筆): "植物病原体の分子生態" 東北大学遺伝生態研究センタ-, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉山 達夫,他: "「生物化学実験法シリ-ズ」光合成酵素系の研究法:細胞から遺伝子まで" 学会出版センタ-,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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