| Project/Area Number |
02304020
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
蚕糸学
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| Research Institution | Nagoya University |
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Principal Investigator |
YAMASHITA Okitsugu Nagoya Univ. Fac. Agric. Professor, 農学部, 教授 (50023411)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Masahiko Univ. Tokyo, Fac. Agric. Professor, 農学部, 教授 (60162020)
MAEKAWA Hideaki Natl. Inst. Health Senior Researcher, 技術部, 室長 (60100096)
OSANAI Minoru Kanazawa Univ. Fac. Sci. Professor, 理学部, 教授 (50113486)
IZUMI Susumu Tokyo Met. Univ. Fac. Sci. assistant, 理学部, 助手 (10145659)
KOGA Atsumi Kyushu Univ. Fac. Agric. Professor, 農学部, 教授 (40038261)
佐々木 卓治 名古屋大学, 農学部, 助手 (20023492)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 1992: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1991: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1990: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | larval serum protein / 30k protein / storage protein l / protease inhibitor / aldolase / sorbitol dehydrogenase / initiatolin / prothoracicotropic hormone / プロテアーゼインヒヒター / 家蚕 / 幼虫特異タンパク質 / 貯蔵タンパク質 / プロテア-ゼインヒビタ / ソルビト-ルデヒドロゲナ-ゼ / イニンアトリン / アルドラ-ゼ / 卵特異タンパク質 / プロテア-ゼインヒビタ- |
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| Research Abstract |
In this project research, 7 proteins which have unique function in silkworms have been selected for the comprehensive elucidation of their primary structure, gene organization and regulation of the gene expression. The Bombyx mori larval serum protein was found to be a new protein which characterizes the larval life. A pupal serum protein, 30k protein, was shown to be biosynthesized by expression of the gene under the influence of the 5^1promoter sequence. The expression of storage protein 1 gene was stimulated by a nuclear proteins of larval fat body, although the biochemical properties of the proteins remain unknown. Protease inhibitors isolated from silkworm hemolymph were demonstrate to be produced by the regulation of insect hormones which stimulate expression of the protease inhibitor gene. There was a rapid change in isozyme pattern of aldolases during embryogenesis of silkworms. Sorbitol dehydrogenese, which was a rate-limiting enzyme for sorbitol degradation at diapause termination, was produced by the transcription of the gene. Initiatolin, a protease responsible for sperm activation was identifies in spermatophore of silkworms and characterized biochemically. A neuropeptide hormone, prothoracicotropic hormone, was mapped to 2.7 of the 22nd linkage group of silkworms, and diapause hormone was to the 11th linkage.
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