| Project/Area Number |
02304021
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
土壌・肥料
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
ISHIZUKA Junji KYUSYU UNIVERSITY, FACULTY OF AGRICULTURE, PROFESSOR, 農学部, 教授 (20193336)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASI Shirou KAGOSHIMA UNIVERSITY, FACULTY OF SCIENCE, PROFESSOR, 理学部, 教授 (60041216)
YAMAMOTO Yukio NAGOYA GAKUIN UNIVERSITY, FACULTY OF FOREIGN LANGUAGE, PROFESSOR, 外国語学部, 教授 (40022561)
TAJIMA Shigeyuki KAGAWA UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (50116894)
FUJITA Kounosuke HIROSHIMA UNIVERSITY,FACULTY OF APPLIED BIOLOGICAL SCIENCE, PROFESSOR, 生物生産学部, 教授 (90002170)
ARIMA Yasuhiro TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, FACULTY OF AGRICULTURE, ASSOCIAT, 農学部, 助教授 (90011973)
庄野 邦彦 東京大学, 教養学部, 教授 (60050457)
南沢 究 茨城大学, 農学部, 助教授 (70167667)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥25,400,000 (Direct Cost: ¥25,400,000)
Fiscal Year 1992: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1991: ¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1990: ¥11,400,000 (Direct Cost: ¥11,400,000)
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| Keywords | Host specificity / Bradyrhizobium / Nod gene / Nodulin / Lac operon / Flavonoid compound / Uricase / R. fredii / 宿主特異性 / ダイズ根粒菌 / グルコサミンテトラサッカライド / カ-リング / nodulin遺伝子 / ウリカ-ゼ / クロ-ニング / 菌体外多糖 / レグヘモグロビン / 共生特異的遺伝子 / ノジュリン遺伝子 / 宿主域の拡大 / フラボノイド処理 / 根毛のカ-リング |
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| Research Abstract |
In relation to host specificity of soybean rhizobia and preferences of soybean for rhizobia, the lectin agglutinating and growth-stimulating the compatible bacterial strain with the Rj_2-cultivar was extracted from seeds of Rj_2 cultivar. The isolated rhizobia from wild and cultivated soybeans and sairatro were classified into 3 nod-groups which corresponded to each host plant. Host specific process was seemed to be the formation of infection thread and not the attachment of rhizobia. The plasmid which has a fragment containing nodulation genes from R. leguminosarum. lac operon and Tn5 were transferred into R. fredii. In the transconjugant CT24, common nod genes interfere with R. fredii nod genes, causing poor nodulation in soybean. Effects of exudates from seeds and roots of cowpea on nodulation for cowpea were examined, and flavonoid commpounds were identified. Nitrate nitrogen uptake in roots was transferred rapidly into the shoots, and hardly transaferred into nodules. The reduction of nitrogen fixing activity by removal of developing pods was due to reduction of removal of fixed nitrogen from nodules. Infected and uninfected cells shared nitrogen fixing process, xanthine oxidase and malate dehydrogenase were detected in the formers, and CO_2 fixing enzyme, uricase and amino acid synthesizing-enzyme were revealed mainly in the latters. One of nodulins, uricase in soybean nodule were examined for the characteristics, and cotyledons were shown to contain a uricase that cross-react with antibodies raised against the nodule uricase. Novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development were isolated and chracterized.
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