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El mice : Seizure mechanisms and their management

Research Project

Project/Area Number 02304045
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionOkayama University

Principal Investigator

MORI Akitane  Okayama Univ. Med. Sch., Professor, 医学部, 教授 (20028434)

Co-Investigator(Kenkyū-buntansha) MIZIKAWA Kiminao  Okayama Univ. Med. Sch., Assistant Professor, 医学部, 講師 (40137154)
SUZUKI Jiro  Toho Univ. Sch. of Med., Professor, 医学部, 教授 (10090408)
SUGAYA Eiichi  Kanagawa Dental Univ., Professor, 歯学部, 教授 (50084710)
YAMAGAMI Sakae  Osaka Univ. Med. Sch., Associate Professor, 医学部, 助教授 (20047004)
MITA Takashi  Univ. of Occupation and Environmental Health Sch. of Med., Professor, 医学部, 教授 (20076988)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1991: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsEl*mice / Poly A^+ RNA / c-fos mRNA / Glutamic Aicd / Aspartic Acid / GABA / Tyrosine / serotonin / トリプトファン / シタロプラム / mRNA異常発現 / NMDAレセプタ- / GABA作動系異常 / 神経伝達物質 / Ca輸送 / baclofen
Research Abstract

All experimental results by our research group are as follows :
1. The amino acid metabolism and gene activity, including poly-A^+ RNA and sodium channel mRNA expression, were unusual at the early postnatal stage in the brain and liver.
2. The expression of c-fos RNA was observed just after convulsion in the brain of seizure experienced El mice.
3. Disturbances of neuronal growth, development and differentiation were observed. Excitatory state of neuronal membrane was detected without any convulsant in the primary curtured neuron.
4. Mechanisms for onset of secondary generalized seizures were studied in the hippocampal neurons.
5. Decreased cell numbers and dendrites, and also muscimol binding sites were observed in the hippocampal region.
6. Epileptogenic abnormalities in the excitatory neurotransmitter binding sites were recognized.
7. Disorders in the GABAergic, noradrenergic, and glutamateric receptors were found in the brain.
8. GABAergic and glutamatergic neuronal systems changed with growth, development or stimulations.
9. Abnormal release of excitatory and inhibitory neurotransmitters was observed in the cerebral and hippocampal slices.
10. Genetically and extraordinally higher level of kynurenine, lower level of dopamine, and other metabolic disorders, were found in the brain.
11. Decreased transport of Ca into blood from the bone was recognized. This decreased availability of Ca may result in a decrease of calmodulin dependent synthesis of amines, which leads to a lowered seizure threshold.
12. El mouse seizures were inhibited by baclofen, a GABA_B receptor agonist, and citalopram, an inhibitor for serotonin re-uptake.
13. El mouse strain has been controlled genetically and microbiologically to keep and to breed and also to be easy available to any epileptologists for further experiments.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Mori,Akitane: "Genetically higher level of guanine and guanosine in the brain of epileptic El mice." Med.Sci.Res.19. 445-446 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitani,Takashi: "Unusual biochemical development of genetically seizure-susceptible El mice." Develop.Brain Res.64. 27-35 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugaya,Eiichi: "Cellular physiology of epileptogenic phenomena and its application of therapy against intractable epilepsy." Comp.Biochem.Physiol.98C. 249-270 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoneda,Yukio: "Neurochemical aspects of the N-methyl-D-aspartate receptor complex." Neuroscience Res.10. 1-33 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Murashima,Yoshiya: "Temporal and Spacial differences during development in GABAergic systems of the El mouce." Jpn.J.Psy.Neurol.45. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki,Jiro: "Inhibition,propagation and generalization of paroxysmal discharges in an epileptic mutant animal." Jpn.J.Psy.Neurol.45. 271-274 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mori,Akitane: "International Perspectives on Self-Regulation and Health" Plenum Press, 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoneda,Yukio: "Neuroreceptor Mechanisms in Brain" Plenum Press, 20 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mori, Akitne: "Genetically higher level of guanine and guanosine in the brain of epileptic El mice." Med. Sci. Res.19. 445-446 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitani, Takashi: "Unusual biochemical development of genetically seizure-susceptible El mice." Develop. Brain Res.64. 27-35 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugaya, Eiichi: "Cellular physiology of epileptogenic phenomena and its application of therapy against intractable epilepsy" Comp. Biochem. Physiol.98C. 249-270 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoneda, Yukio: "Neurochemical aspects of the N-methyl-D-aspartate meceptor complex." Neuroscience Res.10. 1-33 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Murashima, Yoshiya: "Temporal and spacial differences during development in GABAergic systems of the El mouse." Jpn. J. Psy. Neurol.45. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki Jiro: "Inhibition, propagation and generalization of paroxysmal discharges in an epileptic mutant animal." Jpn. J. Psy. Neurol.45. 271-274 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mori, Akitane: "International Perspectives on Self-Regulation and Health" Plenum Press. 11. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoneda, Yukio: "Neuroreceptor Mechanisms in Brain" Plenum Press. 20. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mori,Akitane: "Genetically higher level of guanine and guanosine in the brain of epileptic El mice." Med.Sci.Res.19. 445-446 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mitani,Takashi: "Unusual biochemical development of genetically seizureーsusceptible El mice." Develop.Brain Res.64. 27-35 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sugaya,Eiichi: "Cellular physiology of epileptogenic phenomena and its application of therapy against intractable epilepsy." Comp.Biochem.Physiol.98C. 249-270 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoneda,Yukio: "Neurochemical aspects of the NーmethylーDーaspartate receptor complex." Neuroscience Res.10. 1-33 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Murashima,Yoshiya: "Temporal and spacial differences during development in GABAergic systems of the El mouce." Jpn.J.Psy.Neurol.45. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Suzuki,Jiro: "Inhibition,propagation and generalization of paroxysmal discharges in an epileptic mutant animal." Jpn.J.Psy.Neurol.45. 271-274 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,Akitane: "International Perspectives on SelfーRegulation and Health" Plenum Press, 11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoneda,Yukio: "Neuroreceptor Mechanisms in Brain" Plenum Press, 20 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Osaki,Y.et al.: "Uptake and K^+ーstimulated release of glutamate from cerebral cortical and hippocampal slices in El mice,and dose effect of glutamate" Neurosciences. 16. 561-567 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Higashihara,Y.et al.: "Aspartic acid release from cerebral cortical slices of El mice with high seizure susceptibility" Res.Commun..Chem.Path.70. 155-171 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiramatsu,M.et al.: "Regional excitatory and inhibitory amino acid levels in epileptic El mouse brain." Neurochem.Res.15. 821-825 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sato,K.et al.: "Effect of noncompetitive antagonist (MKー801) of NMDA receptors on convulsions and brain amino acid level in El mice" Jpn.J.Psychiat.Neurol.44. 434-435 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamashita,K.et al.: "Changes in brain thyrotropinーreleasing hormone (TRH) of seizureーprone El mice" Exp.Neurol.108. 71-75 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.et al.: "Tryptophan hydroxylase activities in brain various regions of El mice" Med.Sci.Res.18. 521-522 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mori,A.et al.: "International Perspectives on Selfーregulation and Health" Plenum Publishing Corporation, 12 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ito,M.et al.: "Amino Acids:;Chemistry,Biology and Medicine" ECOM Science Publishers B.V., 12 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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