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Regulation Mechanisms of Cotraction-Relaxation of Muscle

Research Project

Project/Area Number 02304066
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionNagoya University

Principal Investigator

MIHASHI Koshin  Nagoya Univ., Facult.of Sci.Ass.Prof., 理学部, 助教授 (30022594)

Co-Investigator(Kenkyū-buntansha) OBINATA Yakashi  Chiba Univ.Facult.of Sci., 理学部, 教授 (40012413)
MASAKI Tomoo  Kyoto Univ.Facult.of Med.Prof., 医学部, 教授 (60009991)
NONOMURA Yoshiaki  Tokyo Univ. Facult.of Med.Prof., 医学部, 教授 (80009993)
YAMADA Kazuhiro  Ooita Med.College Prof., 医学部, 教授 (20053027)
OOTSUKI Iwao  Kyushu Univ.Facult.of Med., Prof., 医学部, 教授 (70009992)
江橋 節郎  岡崎国立共同研究機構, 生理学研究所, 教授 (10009863)
丸山 工作  千葉大学, 理学部, 教授 (60012267)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1990: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordsactin binding proteins / gizzard muscle / regulatory proteins / caged-ATP / MLCK / connectin / structural repeat / sliding velocity / 調節タンパクの交叉実験 / cagedーATP / 構造の不均一性 / 筋の収縮弛緩 / コフィリン / αーアクチニン / ゲルゾリン / アクトミオシン / アクチン フィラメント
Research Abstract

1. Characterization and Function of Regulatory Proteins
(1) Distribution of cofilin in situ (Obinata). (2) Screening of actomyosin system by means of specific inhibition of "wortmannin" in the variety of cells & functions (Nonomura). (3) Fragmentation of connectin under the influence of Ca ion (Maruyama, Takahashi). (4) Leiotonin in gizzard muscle (Ebashi). (5) Structural repeat in LMM (Ooi). (6) Genetic expression mechanism of M-protein (Masaki).
2. Regulation of Actin-Myosin Interaction
(1) Recovery of Ca-induced tension after recombination of Tn-C from various animal tissues (Ohtsuki). (2) Relaxation of skeletal muscle induced by ATP released from caged-ATP by flash (Yamada). (3) Additivity of sliding velocity of actin-myosin driven by ATP and GTP (Mihashi).

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Toshiro ODA: "Additivity of the sliding velocity of Acto-H.werowyosu in vitro Driveu by ATP and GTP" Pro.Japan Acad., Ser B.69. 1-2 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Tanokura: "Preparation and claralterization of Tropouis,C from Bull frog skeletal Muscle" J.Biochem.112. 514-522 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Horiuti: "Effects of carbon dioxide on tefavic contraction of frog skeletal muscle" J.Pkysiol.453. 247-259 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Kanoh: "Eubacing effect of worfmauuin on muscarivic stimalation of pkospholipase D" Biochem d Bioptys.Res.Comm.188. 510-515 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Toshiro ODA: "Additivity of the sliding velocity of Acto-H-werowgosin in vitro Driven by ATP and GTP" Pro-Japan Acad.Ser B.69. 1-2 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Tanokura: "Preparation and Characterization of Troponin, C from Bull frog skeletal wuscle" J.Biodun. 112. 514-522 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.HORIUCHI: "Effects of carbon dioxide on tetanic contraction of forg skeletal wuscle" J.Physiol. 453. 247-259 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Kanoh: "Enlecenciug eHect of wortwaunin on wuscarinic stiwulation of phospholipase D" Biochem + Biopleus. Res.Cowni.188. 510-515 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Toshiro,ODA: "Additivity of the Sliding Velocity of Acto-H-meromyosin in vitro Drived by ATP and GTP" Proc.Japan Acad.,ser.B. 69. 1-2 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Tanokura: "Preparation and Characterization of Troponin C from Bullfrog Skeletal Muscle" J.Biochem.112. 514-522 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Horiuti: "Effects if Carbom dioxide on tetamic contract fion of frog skeletal muscle" J.Physiol.453. 247-259 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Kamoh: "Enhamcing effect of Wortmammim on musearimic stimulation of phospholipass D" Biochem.d Bioptugs.Rus.Comm. 188. 510-515 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] N.Suzuki,and K.MIHASHI: "Binding Mode of Cytochalasin B to F‐actin is Altered by Lateral Binding of Regulatory Proteins." J.Biochem.109. 19-23 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Suzuki,and K.MIHASHI: "The High‐affinity Binding of Cytochalasin B to the B‐end of F‐actin loses its Inhibition Effect on the Subunit Exchange When the Bound Nucleotide is ADP" J.Biochem.110. 514-519 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hamada,Y.: "Distinct vascular and intestinal smooth muscle myosin heavy chain mRNA are encoded by a singlecopy gene in the chicken" Biochem.Biophys,Res.Commun.(1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Abe,H.: "Sequence of cDNAs encoding actin depolymering factor and cofilin of embryonic chicken skeletal musde:two functionally distinct actin regulatory proteins" Biochemistry. 29. 7420-7425 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuwayama,H.: "Porcine skeletal Muscle Dystrophin" Proc.Japan Acad.66(B). 145-150 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Maruyama,K.: "βーActinin is equivalent to CapーZ protein" J.Biol.Chem.265. 8712-8715 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kometani,K.: "Mechanical and energetic properties of dystrophic (mdx) mouse muscle" Jpn.J,Physiol.40. 541-549 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Suzuki,N.: "Binding mode of cytochalasin B to Fーaetin is altered by lateral binding of regulatory proteins" J.Biochem.109. 19-23 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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