| Project/Area Number |
02304068
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物物性学
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| Research Institution | Nagoya City University |
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Principal Investigator |
NAKANISHI Mamoru Nagoya City Univ. Fac. Pharm. Sci. Professor, 薬学部, 教授 (90090472)
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| Co-Investigator(Kenkyū-buntansha) |
美宅 成樹 東京農工大学, 工学部, 助教授 (10107542)
KUSUMI Akihiro Tokyo Univ. Fac. Culture Assist Prof., 教養学部, 助教授 (50169992)
KIRINO Yutaka Kyusyu Univ. Fac. Pharm. Sci. Professor, 薬学部, 教授 (10012668)
KINOSITA Kazuhiko Keio Univ. Fac. Sci. Professor, 理工学部, 教授 (30124366)
KASAI Michiki Osaka Univ. Fac. Eng. Professor, 基礎工学部, 教授 (40022595)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1991: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1990: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Confocal Microscope / Calcium Signal / Time-resolved Microscope / Ion Channel / Ca^<2+>-ATPase / Dual-view Microscope / Membrane Fluidity / Immune Responses / カルシウムATPase / パルスレ-ザ顕微鏡 / 蛋白質前駆体 / 全重水素化リン脂質 / クロライドチャネル / 血小板 / 金コロイド |
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| Research Abstract |
We have studied the molecular mechanisms of bio-communication on the membrane. First, we investigated new methods for fluorescence microscopes. Dr. Nakanishi investigated a confocal fluorescence microscope and studied the calcium signals in the immune responses. He found that the receptormediated calcium signals were transferred not only to the cytoplasm but also to the nucleus. Dr. Kinosita investigated a dual-view microscope and Dr. Kusumi investigated a fluorescence life-time imaging microscope. By thses methods they studied bio-communications on the membrane surfaces. Dr. Kitagawa studied platelet membrane fluidity by fluorescence method and found asymmetry of membrane fluidities. Dr. Kasai and Dr. Kirino studied ion channels and they discovered roles of ion channels in bio-communications. Dr. Inagaki and Dr. Endo determined the structures of proteins which work in membrane bio-communications. Dr. Hatta and Dr. Mitaku proposed new models for dynamic structures of proteins and lipids which regulated the bio-communication on the membranes. From these results we demonstrated a new picture for dynamics of bio-communications on the membranes.
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