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Establishment of X-Ray Structure Analysis System for Protein Crystals by Multiwavelength Anomalous Diffraction Methods.

Research Project

Project/Area Number 02402051
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionUniversity of Tokyo

Principal Investigator

SATOW Yoshinori  University of Tokyo Faculty of Pharmaceutical Sciences Professor, 薬学部, 教授 (30150014)

Co-Investigator(Kenkyū-buntansha) NAKASAKO Masayoshi  University of Tokyo Faculty of Pharmaceutical Sciences Assistant, 薬学部, 助手 (30227764)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥24,500,000 (Direct Cost: ¥24,500,000)
Fiscal Year 1991: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1990: ¥22,200,000 (Direct Cost: ¥22,200,000)
KeywordsProtein Crystallography / Multiwavelength Anomalous / X-Ray Crystallography / Diffraction Intensity / Immunoglobulin / Fv Fragment / Synchrotron Radiation / Anomalous Scattering / アミラ-セ阻害剤 / ダンシルリジン
Research Abstract

The objective of this. research project is to establish a system for direct and rapid structure determination of protein crystals by use of tunable and intense synchrotron X-radiation.
First, a dead time correction method for scintillation detectors and automatic optimization procedures for alignment of the X-ray optical components were newly developed for the diffraction intensity measurement system, which consists of the double-crystal monochromator, focussing mirror, and horizontal-type four-circle diffractometer, installed at Photon Factory, National Laboratory for High Energy Physics. The measurement system improved so as to accommodate these procedures is applicable to four-circle diffractometry as well as to oscillation methods, and provides excellent-quality diffraction data at any practical wavelengths. The system has been in open use to various synchrotron experiments. Next, a kappa-axis diffractometer-with a sealed X-ray tube was equipped with a portable crystal-cooling unit … More which decreases radiation damages on protein crystals. Finally, computer programs for processing diffraction data measured at several wavelengths as well as for direct phasing of crystal structure factors were developed. The programs combined with structure refinement, graphics, and other programs were built into a complete system for multiwavelength anomalous diffraction analyses.
Crystal Structure of an Fv fragment from mouse anti-dansyllysine immunoglobulin IgG2a has been determined at 2.5 * resolution. The structure clearly elucidates its complementarity determining regions. Structure refinements of the native crystal and a dansyllysine complex crystal are in progress at hislier resolution. Furthermore, crystal structures of ferous spincrossover complexes as well as those of aspartaine IA and IB forms liave been solved in the course of performance tests of the measurement and proprain systems.
The systems established in this research project are widely applicable to crystal structure studies of proteins and others. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] 佐藤 能雅: "シンクロトロン放射光と蛋白質結晶解析" 蛋白質核酸酵素. 37. 567-579 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 能雅: "タンパク質の活性、機能と進化の情報を与える立体構造" 現代化学. 245. 24-31 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshinori Satou: "Optimized Anomalous Dispersion Studies" Acta Crystallographica. A46. C-15 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wei Yang: "Structure of Ribonuclease H Phased at 2Å Resolution by MAD Analysis of Selenomethionyl Protein" Science. 249. 1398-1405 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Janet L.Smith: "Multiwavelength Anomalous Diffraction Analysis of a Large Protein" Proc.of Isomorphous Replacement and Anomalous Scattering.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中迫 雅由: "Crystal Structure of anti・Dansyllysine Jmmunoglobulin FvーFragment"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wei Yang: "Determination of Escherichia coli Selenomethionyl RNase at 2.0 Å Resolution." Acta Crystallographica. A46. C-90 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Croig M.Ogata: "1.8Å X-ray Crystal Structure of Chromomycin-DNA Complex" Acta Crystallographica. A46. C-146-C-147 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Janet L.Smith: "Multiwavelength Anomalous Diffraction Experiments on Glutamine PRPP Amidotrasterase" Acta Crystallographica. A46. C-17 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshio Takeuchi: "Refined Crystal Structure of the Complex of Subtilisin BPN' and Streptomyces Subtilisin Inhibitor at 1.8Å Resolution" J,Mol.Biol.221. 309-325 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 能雅: "回折法による結晶構造解析の基礎(分担は,「放射光の利用ー放射光X線の特徴と主体高分子の解析pp.111〜127)" 日本結晶学会, 127 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshinori Satow: "Optimized Anomalous Dispersion Studies." Acta Crystallographica A46. C-15. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wei Yang, W. A. Hendrickson, R. J. Crouch, & Y. Satow: "Structure of Ribonuclease H Phased at 2 * Resolution by MAD Analysis of Selenomethionyl Protein." Science. 249. 1398-1405 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Janet L. Smith, E. J. Zaulec, J. -P. Wery, & Y. Satow: "Multiwavelength Anomalous Diffraction Analysis of a Large Protein." Proc. of Isomorphous Replacement and Anomalous Scattering.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshio Takeuchi, Y. Satow, K. T. Nakamura, & Y. Mitsui: "Refined Crystal Structure of the Complex of Subtilisin BPN' and Streptomyces Subtilisin Inhibitor at 1.8 * Resolution" J. Molec. Biol.221. 309-325 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshinori Satow: "Use of Synchrotron Radiation - Characteristics of Synchrotron Radiation and Macromolecular Crystallography." Fundamentals in Crystal Structure Analysis by Diffraction Methods, Japanese Crystallographic Society. 111-127 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshinori Satow: "Protein Crystallography and Synchrotron Radiation." Tanpakushitsu Kakusan Kouso. 37. 567-579 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshinori Satow: "Crystal Structures give Information about Activities, Functions, and Evolutions of Proteins." Chemistry Today (Gendaikagaku). 245. 24-31 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Janet L. Smith, E. J. Zaulec, R. L. Switzer, Y. Satow, & J. -P. Wery: "Multiwavelength Anomalous Diffraction Experiments on Glutamine PRPP Amidotransferase." Acta Cryst. A46. C-17. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wei Yang, W. A. Hendrickson, R. J. Crouch, & Y. Satow: "Determination of Escherichia coli Selenomethionyl RNase H at 2.0 * Resolution." Acta Cryst. A46. C-90. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Craig M. Ogata, W. A. Hendrickson, Y. Satow, & X. Gao: "1.8 * X-ray Crystal Structure of a Chromomycin-DNA Complex." Acta Cryst. A46. C-146-147. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayoshi Nakasako, Y. Satow et al.: "Crystal Structure of Mouse Anti-Dansyllysine Immunoglobulin Fv Fragment"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 能雅: "シンクロトロン放射光と蛋白質結晶解析" 蛋白質核酸酵素. 37. 567-579 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshinori Satow: "Optimized anomalaus Dispersion Studies" Acta Crystallographica. A46. C-15 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Janet L.Smith: "Multiwavelength Anomalous Diffraction Analysis of a Large Protein" Proc.of Isomorphous Replacement and Anomalaus Scatlering.

    • Related Report
      1991 Annual Research Report
  • [Publications] Wei Yong: "Structure of Ribonuclease H Phased at 2A Resolution by MAD Analysis of Selenomethionyl Protein" Science. 249. 1398-1405 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 中迫 雅由: "Crystal Stracture of AntiーDansyllysine Immunoglobulin FvーFragment"

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 能雅: "タンパク質の活性,機能と進化の情報を与える立体構造" 現代化学. 245. 24-31 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshinori Satow(佐藤 能雅): "Optimized Anomalous Dispersion Studies." Acta Crystallographica. A46. C-15 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Wei Yang: "Structure of Ribonuclease H Phased at 2A^^° Resolution by MAD Analysis of the Selenomethionyl Protein." Science. 249. 1398-1405 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤 能雅: "X線構造解析による生体高分子の立体構造研究" 現代化学. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Janet L.Smith: "Multiwavelength Anomalous Diffraction Experiments on Glutamate PRPP Amidotransferase." Acta Crystallographica. A46. C-17 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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