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Molecular bases of initial processes for formation of crown gall tumor

Research Project

Project/Area Number 02404003
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionNagoya University

Principal Investigator

MACHIDA Yasunori  Nagoya University,Faculty of Science,Department of Biology,Professor, 理学部, 教授 (80175596)

Co-Investigator(Kenkyū-buntansha) USAMI Shoji  Nagoya University,Faculty of Science,Department of Biology,Research Associate, 理学部, 助手 (80242816)
YOSHIOKA Yasusi  Nagoya University,Faculty of Science,Department of Biology,Research Associate, 理学部, 助手 (60202397)
丹羽 康夫  静岡県立大学, 食品栄養科学部, 助見 (00222191)
高橋 陽介  東京大学, 理学部, 助手 (90183855)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥32,100,000 (Direct Cost: ¥32,100,000)
Fiscal Year 1992: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1991: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 1990: ¥18,900,000 (Direct Cost: ¥18,900,000)
KeywordsAgrobacterium / T-DNA / Crown gall tumor / Plants / Ti plasmid / vir genes / TーDNA / クラウンゴ-ル腫瘍
Research Abstract

(1)Mechanisms of plant signal perception by Agrobacterium We have demonstrated that the periplasmic domain and TM2 of a VirA sensor protein are involved in recognition of sugar signal, but not in that of phenolic signal. We have isolated chvE mutations which restore enhancement of vir induction by sugars in the vir A mutant which shows a sugar-non-responsive phenotype. This result indicates that the ChvE protein physically interacts with the VirA protein in the signal pathway mediated by these proteins. (2)vir genes responsible for movement of T-DNA from Agrobacterium to plant cells We have that products of virB and virD4 genes are strictly required for transfer of T-DNA from Agrobacterium to plant cells and that a product of virE2 markedly potentiates this process, but is not essential. (3)Integration of T-DNA into plant nuclear DNA We have shown that in each case of two T-DNA-transformed tobacco tumors, significant sequence homology is present between the region adjacent to the integration target site and both external regions of the T-DNA breakpoints. These findings suggest that sequence homology between the incoming T-DNA and plant chromosomal DNA has an important function in T-DNA integration. We also found that members of a new family of SINE (short interspersed DNA element), which is designated as the TS family, were present in DNA region adjacent to T-DNA integration targets of tobacco chromosomes. A relationship between integration events of T-DNA and the Ts family remains to be proved.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Hiroharu Banno: "Isolation and characterization of cDNA clones encoding protein kinases of Nicotiana tabacum" Journal of Plant Research. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasunori Machida: "Molecular interactions between Agrobacteium and plant cells in Molecular Genetics of Plant-Microbe Interactions" Kluwer Academic Publishers,Netherlands, 11 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasushi Yoshioka: "Mechanism of transfer and integration of T-DNA into plant chromosomes in Molecular Machanisms of Bacterial Virulence" Kluwer Academic Publishsers,Netherlands, (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroharu Banno: "Isolation and characterization of cDNA clones encoding protein kinases of Nicotiana tabacum" Journal of Plant Research. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasunori Machida: Kluwer Academic Publishers,Netherlands. Molecular interactions between Agrobacterium and plant cells in Molecular Genetics of Plant-Microbe Interactions, (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasushi Yoshioka: Kluwer Academic Publishers,Netherlands. Mechanisms of transfer and integration of T-DNA into plant chromosomes in Molecular Mechanisms of Bacterial Virulence, (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroharu Banno: "Isolation and characterization of cDNA clones encoding kinases of Nicotiana tabacum" Journal of Plant Research. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasunori Machida: "Molecular interactions between Agrobacterium and plant cells in Molecular of Plant-Microbe Interactions" Kluwer Academic Publishers,Netherlands, 11 (1996)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasushi Yoshioka: "Mechanism of transfer and integration of T-NDA into plant chromosomes in Molecular Mechanisms of Bacterial Virulence" Kluwer Academec Publishers,Netherlands, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shigehisa Okamoto: "Localization and orientation of the VirD4 protein of Agrobacterium tumefaciens in the cell membrane" Molecular General Genetics. 228. 24-32 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hitoshi Onouchi: "Operation of an efficient siteーspecific recombination system of Zygosaccharomyces rouxii in tobacco cells" Nucleic Acids Research. 19. 6373-6378 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 吉岡 泰: "TーDNAの植物染色体組込みに関する最近の知見" 植物細胞工学. 3. 458-463 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shogo Matsumoto: "Integration of Agrobacterium TーDNA into a tobacco chromosome:Possible involvement of DNA homology between TーDNA and plant DNA" Molecular General Genetics. 224. 309-316 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nobuyoshi Shimoda: "Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides" Proceedings of National Academy of Science,USA. 87. 6684-6688 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ken Matsuoka: "Vacuolar targetting and post translational processing of the precursor to the sweet potato tuberousーroot storage protein in heterologous plant cells" Journal of Biological Chemistry. 265. 19750-19757 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigehisa Okamoto: "Membrane localization and topology of Agraobacterium tumefaciens VirD4 protein" Submitted to Molecular General Genetics.

    • Related Report
      1990 Annual Research Report
  • [Publications] 松本 省吾: "植物形質転換法" 現代化学. 6. 25-30 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 松本 省吾: "Tiプラスミドベクタ-による植物への遺伝子導入" 蛋白質 核酸 酵素. 35. 2476-2489 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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