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Cell biology analyses on the synthesis and localization of the proteins in fungal cells

Research Project

Project/Area Number 02404005
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 植物形態・分類学
Research InstitutionNagoya University School of Medicine

Principal Investigator

TANAKA Kenji  School Medicine Professor, 医学部, 教授 (70013315)

Co-Investigator(Kenkyū-buntansha) AKASHI Tomohiro  Sch.Medicine Res. Assoc., 医学部, 助手 (00222513)
KANBE Toshio  School Medicine Assist. Prof., 医学部, 講師 (50093018)
HOMMA Michio  Faculty Science Assoc. Prof., 理学部, 助教授 (50209342)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥22,000,000 (Direct Cost: ¥22,000,000)
Fiscal Year 1992: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1991: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1990: ¥16,700,000 (Direct Cost: ¥16,700,000)
KeywordsYeast / Candida albicans / Schizosaccharomyces pombe / proteinase / secretion / actin / immunocytochemistry / electron microscopy / Yest / Candia albicans / 免疫組織化学 / 電子顕微鏡 / プロテア-ゼ / 酵素誘導 / 免疫電子顕微鏡法
Research Abstract

1. Biochemical studies on the detection of intracellular forms of secreted aspartic proteinase in Candida albicans. C. albicans secretes acid proteinase (EPR) in the medium which contains bovine serum albumin as a sole source of nitrogen. By immunoblotting with anti-EPR serum, 43 and 45 kDa proteins were detected in the cytoplasmic fraction, which recovered from the different membrane fractions. 45 kDa would be a precursor of the secreting 43 kDa protein. 2. Ultrastructure studies on the proteinase secreting cells by alkaline bismuth staining and immunocytochemistry. We found many flocculent granules at the cell periphery of the proteinase-secreting cells, but the granules were not specific for the secretion. This was deduced from observations of alkaline bismuth staining, of effects of a secretion inhibitor Brefeldin A, and of immunoelectron microscopy using anti-EPR serum. 3. Biochemical studies on the induction of extracellular proteinase (EPR) in Candida albicans. It was shown that EPR was induced without the addition of nitrogen source so long as glucose as a carbon source is present, and not induced at a pH greater than 6.0 irrespective of the presence of nitrogen source. the results suggested EPR production and germ tube formation play different roles in the invasive process. 4. Studies on the behavior of F-actin in Schizosaccharomyces pombe by fluorescent and electron microscopy. (i) Starvation in distilled water dispersed the actin dots localized at the cell ends into thick patches and cables throughout the cytoplasm. (ii) Cytochalasin A inhibited the appearance of actin ring at a cell equator before septum formation and cytoplasmic vesicles and dictyosomes were altered in to dense bodies in the cytoplasm. Actin network maintains the organization of the secretory pathway involving dictyosomes and vesicles.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] M.Homma,T.Kanbe H.Chibana,K.Tanaka: "Detection of intracellular forms of secreted aspartic proteinase in Candida albicans." Journal of General Microbiology. 138. 627-633 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Akashi,M.Homma T.Kanbe,K.Tanaka: "Ultrastructure of proteinase-secreting cells of Candida albicans studied by alkaline bismuth staining & immunocytochemistry" Journal of General Microbiology.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Homma,H.Chibana K.Tanaka: "Induction of extracellular proteinase (EPR) in the dimorphic yeast Candida albicans." Journal of General Microbiology.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Kanbe,T.Akashi K.Tanaka: "Change of the distribution of F-actin in the fission yeast Schizosaccharomyces pombe by arresting the growth in distilled water:Studies with fluorescent & EM."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Kanbe,T.Akashi K.Tanaka: "Effect of cytochalasin A on actin distribution of the fission yeast Schizosaccharomyces pombe studied by fluorescent and electron microscopy"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Homma,M. Kanbe,T. Chibana,H. Tanaka,K: "Detection offintracellular forms of secreted aspartic proteinase in Candida albicans" J.General Microbiology. 138. 627-633 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Akashi,T. Homma,M. Kanbe,T. Tanaka,K: "Ultrastructure of proteinase-secreting cells of Candida albicans studied by alkaline bismuth staining and immunocytochemistry." J.General Microbiology. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Homma,M. Chibana,H. Tanaka,K: "Induction of extracellular proteinase (EPR) in the dimorphic yeast Candida albicans." J.General Microbiology. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanbe,T. Akashi,T. Tanaka,K: "Changes of the distribution of F-actin in the fission yeast Schizosaccharomyces pombe by arresting the growth in distilled water: Studies with fluorescent and electron microscopy."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanbe,T. Akashi,T. Tanaka,K: "Effect of cytochalasin A on actin distribution of the fission yeast Schizosaccharomyces pombe studied by fluorescent and electron microscopy."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Homma,T.Kanbe,H.Chibana,K.Tanaka: "Detection of intracellular from of secreted aspartic proteinase in Candida albicans." Journal of General Microbiology. 138. 627-663 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Akashi,M.Homma,T.Kanbe,K.Tanaka: "Ultrastructure of proteinase-secreting cells of Candida albicans studied by alkaline bismuth staining & immunocytochemestry" Journal of General Microbiology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Homma,H.Chibana,K.Tanaka: "Induction of extracellrlar proteinase(EPR)in the dimorphec year Candia albicans" Jounal of General Microbiology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 本間 道夫: "Candida albicansの菌体外プロテア-ゼ" 細胞. 22. 54-57 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Homma,T.Kanbe,H.Chibana,K.Tanaka: "Detection of intracellular forms of secreted aspartic proteinase in Candida albicans" Journal of General Microbiology. 138. 627-633 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Akashi,M.Homma,K.Tanaka: "Intracelluar localization of the acid proteinase in Candia albicans cells studied by immunoelectron microscopy" Journal of General Microbiology In preparation.

    • Related Report
      1991 Annual Research Report
  • [Publications] 本間 道夫: "Candida albicansの菌体外プロティナ-ゼ" 細胞. 22. 54-57 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Homma,M.: "Induction and intracellular location of extracellular proteinase in Candida albicans"

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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