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Molecular pharmacological investigation on pharmaco-mechanical process in smooth muscles.

Research Project

Project/Area Number 02404024
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKyushu University

Principal Investigator

KURIYAMA Hirosi  Kyushu University, Faculty of Medicine, Professor, 医学部, 教授 (40037495)

Co-Investigator(Kenkyū-buntansha) ITOH Takeo  Kyushu University, Faculty of Medicine, Lecturer, 医学部, 講師 (70159888)
KITAMURA Kenji  Kyushu University, Faculty of Medicine, Lecturer, 医学部, 講師 (30112345)
ITO Yushi  Kyushu University, Faculty of Medicine, Assistant Professor, 医学部, 助教授 (80037506)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥22,200,000 (Direct Cost: ¥22,200,000)
Fiscal Year 1991: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1990: ¥17,600,000 (Direct Cost: ¥17,600,000)
Keywordsvascular smooth muscle / histaminergic receptor current / purinergic receptor current / GTP-binding protein / patch clamp / voltage-dependent Ca current / nonselective cation channel / ヒスタミン受容体電流 / プリン受容体電流 / パッチ固定法 / 電位依存性Ca電流 / 非選択的カチオンチャネル / 薬物ー収縮連関機構 / Ca transient測定法 / 受容体活性化 / 電位依存性Caチャネル / 百日咳毒素
Research Abstract

TO clarify the underlying mechanism of pharinaco-mechanical coupling in vascular smooth muscle, features of the receptor-operated ion channel, effects of second messengers and mechanical responses were investigated. We clarified distributions of at least two different purinergic II receptors (PII_A and PII_B) and features of both channel currents differed. The activations of PII_A and PII_B receptor produced the fast shortlasing and slow sustained inward currents, respectively. The latter was sensitive to GTP and GTPgammaS and enhanced the current amplitude. This action was blocked by GDPbetaS. ATP at low concentrations enhanced the voltage-dependent Ca channel but high concentrations of ATP inhibited this current. This inhibition was accelerated by GTP and GTPgammaS, and prevented by GDPbetaS. Histamine also modified the ionic channel through different three subtypes, i. e. the H_1 receptor activation produced the non-selective cation channel and the H_3 receptor activation inhibited the release of transmitter (mainly noradrenaline and ATP from nerve terminals and also accelerated the voltage-dependent Ca channel. Protein kinase C independent acceleration), but the H_1-induced receptor operated nonselective cation channel was accelerated. In addition, activations of the H_2 receptor by histamine accelerated the cyclic AMP synthesis. Using fura-2 and patch-clamp procedures, when voltagedependent Ca channel and Ca transient were simultaneously recorded, amplitudes of the inward current were enhanced the Ca transient in a concentration dependent manner, this channel activity was modulated by extra- and intra-cellular Na. Presumably, in vascular smooth muscle cells, Na-Ca exchange diffusion mechanism may contribute for determine the cytosolic Ca.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Takeo Itoh: "Roles of protein kinase C on the mechanical acivity of vascular smooth muscles." American Journal of Hypertension. 3. 215s-219s (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junko Kajikuri: "Inhibitory action of α-human atrial natriuretic peptide on noradrenaline-induced synthesis of myo-inositol 1,4,5-trisphosphate in the smooth muscle cells of rabbit aorta" British Journal of Pharmacology. 99. 536-540 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kohji Okabe: "Actions of cromakalim on ionic currents recorded from single smooth muscle cells of the rat portal vein." The Journal of Pharmacology&Experimental Therapeutics. 252. 832-839 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shunichi Kajioka: "Mechanisms of vasodilatation induced by potassium-channel acivators." Clinical Science. 81. 129-139 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Zhiling Xiong: "ATP activates cationic currents and modulates the calcium current through GTP-binding protein in rabbit portal vein." Journal of Physiology. 440. 143-165 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shunichi Kajioka: "Guanosine diphosphate activates an adnosine 5'-triphosphate-sensitive K^+ channel in the rabbit portal vein." Journal of Physiolgoy. 444. 397-418 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Kitamura: "Effects of nifedipine of the calcium channel in the smooth muscle cell membranes.in "Recent advances in calcium channela and calcium antagonists"" eds by K.Yamada&S.Shibata Pergamon Press,New York, 25-32 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Zhiling Xiong: "Actions of endogenous and exogenous ATP on vascular smooth muscle cell membranes.in "Role of adnosine and adenine nucleotides in the biological system"" eds by S.Imai & M.Nakazawa Elsevier, 398-406 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeo Itoh: "Roles of protein kinase C on the mechanical activity of vascular smooth muscles." American Journal of Hypertension. 3. 215Z-219S (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junko Kajikuri: "Inhibitory action of alpha-human atrial natriuretic peptide on noradrenaline-induced synthesis of myo-inositol 1, 4, 5-trisphosphate in the smooth muscle cells of rabbit aorta." British Journal of Pharmacology. 99. 536-540 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kohji Okabe: "Actions of cromakalim on ionic currents recorded from single smooth muscle cells of the rat portal vein." The Journal of Pharmacology & Experimental Therapeutics. 252. 832-839 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shunichi Kajioka: "Mechanisms of vasodilatation induced by potassium-channel acivators." Clinical Science. 81. 129-139 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Zhiling Xiong: "ATP activates cationic currents and modulates the calcium current through GTP-binding protein in rabbit portal vein." Journal of Physiology. 440. 143-165 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shunichi Kajioka: "Guanosine diphosphate activates an adnosine 5'-triphosphate-sensitive K^+ channel in the rabbit portal vein." Journal of Physiology. 444. 397-418 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Kitamura: "Effects of nifedipine of the calcium channel in the smooth muscle cell membranes." "Recent advances in calcium channels and calcium antagonists". Pergamon Press. 25-32 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Zhiling Xiong: "Actions of endogenous and exogenous ATP on vascular smooth muscle cell membranes." "Role of adnosine and adenine nucleotides in the biological system". Elsevier. 398-406 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Zhiling Xiong: "Pinacidil ingibits the rynodineーsensitive outward current and glibenclamide antagonizeds its action in cells from the rabbit portal vein." British Journal of Pharmacology. 102. 788-790 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takeo Itoh: "Effects of pinacidil on contractile proteins in high K^+ーtreated intact,and in βーescinーtreated skinned smooth muscle of the rabbit mesentric artery." British Journal of Pharmacology. 103. 1697-1702 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shinichi Ito: "Effects of lemakalim on changes in Ca^<2+> concentration and mechanical activity induced by noradrenaline in the rabbit mesenteric artery." British Journal of Pharmacology. 104. 227-233 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shunichi Kajioka: "Mechanisms of vasodilatation induced by potassiumーchannel acivators." Clinical Science. 81. 129-139 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Zhiling Xiong: "ATP activates cationic currents and modulates the calcium current through GTPーbinding protein in rabbit portal vein" Journal of Physiology. 440. 143-165 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shunichi Kajioka: "Guanosine diphosphate activates an adnosine 5'ーtriphosphateーsensitive K^+ channel in the rabbit portal vein." Journal of Physiology. 444. 397-418 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kenji Kitamura: "Characteristics of vascular smooth muscle cell membrane and their modifying factors.in “Coronary Circulation in Physiological and Pathophysiological States"" eds by M.Nakamura & P.M.Vanhoutte SpringerーVerlag, 125-156 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Zhiling Xiong: "Actions of endogenous and exogenous ATP on vascular smooth muscle cell membranes.in “Role of adnosine and adenine nucleotides in the biological system"" eds by S.Imai & M.Nakazawa Elsevier, 398-406 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Itoh: "Roles of protein kinase C on the mechanical activity of vascular smooth muscles." American Journal of Hypertension. 3. 215S-219S (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Kajikuri: "Inhibitory action of αーhuman atrial natriuretic peptide on noradrenalineーinduced synthesis of myoーinositol 1,4,5ーtrisphosphate in the smooth muscle cells of rabbit aorta." British Journal of Pharmacology. 99. 536-540 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.D.Gunasekera: "The influence of thyroid states upon responses of the rat aorta to catecholamines." British Journal of Pharmacology. 99. 541-547 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Okabe: "Actions of cromakalim on lonic currents recorded from single smooth muscle cells of the rat portal vein." The Journal of Pharmacology and Experimental Therapeutics. 252(2). 832-839 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Kitamura: "“Effects of nifedipine of the calcium channel in the smooth muscle cell membrtanes."RECENT ADVANCES IN CALCIUM CHANNELS AND CALCIUM ANTAGONISTS 25ー32" ed by K.Yamada,S.Shibata PERGAMON PRESS,New York, 176 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Kitamura: "“Similarity and diversity of electrical activity in vascular smooth muscles."Progress in Clinical and Biological Research 327;257ー276" ed by N.Sperelakis&J.D.Wood WileyーLiss,New York, 849 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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