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Cell biological studies on cell-cell, cell-matrix interaction in bone metabolism.

Research Project

Project/Area Number 02404071
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNiigata University.

Principal Investigator

OZAWA Hidehiro  Niigata University, School of Dentistry, Professor., 歯学部, 教授 (60018413)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Hiroaki  Niigata University, School of Dentistry, Research Associate., 歯学部, 助手 (50227930)
IRIE Kazuharu  Niigata University, School of Dentistry, Research Associate., 歯学部, 助手 (70223352)
EJIRI Sadakazu  Niigata University, School of Dentistry, Associate Professor., 歯学部, 助教授 (40160361)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥26,100,000 (Direct Cost: ¥26,100,000)
Fiscal Year 1991: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1990: ¥21,700,000 (Direct Cost: ¥21,700,000)
KeywordsOsteoclasts / Osteoblasts / Calcitonin / Vitamine D3 Receptor / Carbohydrate chain / Heparan sulfate proteoglycan / Osteocytes / Intracellular Calcium / 細胞間相互作用 / カテプシン / 骨吸収 / 細胞内Ca / レ-ザ顕微鏡 / 細胞骨格
Research Abstract

We conducted 6 series of experiments on the correlation between osteoclasts and osteoblasts : 1) Changes in Ca concentration in osteoclasts was determined by fluorescence-labeling of Ca on single cells, and the effect of calcitonin on Ca movement using an Olympus OSP-3. Ca movement within osteoclasts was transiently enhanced by CT but rapidly decreased, after which the cells recovered their functional activities. The experiment was repeated on osteoclasts cultured on bone tissue, then the osteociasts were also submitted to a 2-dhnentional analysis using an Olympus CASARUS. Ca transport pathway within osteociasts and its inhibition by CT was evidenced. 2) Changes induced by CT on osteociast cytoskeleton was assessed with an Olympus IMT2-21CA3, by observing the changes in the localization of actin filametns and intermediate filaments occurring after addition of CT. 3) To clarify the differentiation of osteoclastic multinuclear giant cells (MNGCs), we cocultured osteoblasts and spleen cel … More ls in the presence of vitamin D3. The PMNGCs thus formed were TRACPasepositive and expressed CT receptors, which lead to consider them as osteociastic. Moreover, we found that a direct cell-to-cell contact with osteoblasts was required in the formation of osteoclastic MNGCs. 4) We also demonstrated by immunocytochemistry in vitro and in vivo that VDR was densely localized in the nucleus, especially in the heterochromatin, and that VDR was transported within the cytoplasm over and along microtubules to reach the nucleus. 5) We studied the interaction between differentiation/activation of osteoclasts and osteoblasts by lectin histochemistry and immunocytochemistry. Reaction of ConA, PNA, WGA, UEA-1 and MPA was intense on the membrane surface of osteoclast and osteoclasts where the two types of cells were in contact each other. The contact surface was also strongly heparan sulphate proteoglycan-positive. These two findings suggest an involvement of carbohydrate chains in the intercellular recognition between osteoclasts and osteoblasts, and to the local reservoir of growth factors and cytokines. 6) We attempted to clarify the inter-relationship between osteoclasts and osteocytes by cytochemistry, whereby we found that the latter cells enters in a cell-ceil interaction with the former : Osteocytes could promote bone resorption by this mechanism. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] 小澤 英浩: "骨代謝のしくみと骨の細胞群" 診断と治療. 78. 2107-2111 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小澤 英浩: "Bone Cellsの微細構造と機能" 日本臨牀. 12. 2736-2741 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mohri,T.,K.Hanada & H.Ozawa.: "Coupling phenomena between bone resorption and formation on bone remodeling in orthodontic tooth movement." J.Bone Mineral Metab.9. 57-69 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 入江 美華,入江 一元,中村 浩彰,江尻 貞一,小澤 英浩: "破骨細胞の分化に関する微細構造学的・オ-トラジオグラフィ-的研究" 日本骨代謝学会誌. 9. 47-53 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Amizuka,N and Ozawa,H: "Intracellular localization and translocation of 1 α,25-dihydroxyvitamin D receptor in osteoblasts." Arch.Histol.Cytol.55. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura,H and Ozawa,H.: "Characteristic localization of carbohydrates in osteoclasts by lectin cytochemistry." Bone. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ejiri,S & H.Ozawa.: "Aerospace Science" University Research Center,Nihon University, 615 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小澤 英浩,江尻 貞一,中村 浩彰,入江 一元,入江 美華,網塚 憲生: "骨代謝調節因子ー最近の進歩" 羊土 社, 189 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ozawa H, Irie M, Nakamura H, Ejiri S.: "Autoradiographic, fine structural and cytochemical relationship between osteoclastic and osteoblastic cells in bone remodeling" Proceedings of the Third International Symposium on osteoporosis. 2096-2101 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Irie K, Ozawa H.: "Relationships between tooth eruption, occlusion and alveolar bone resorption : histochemical and cytological studies of bone remodeling on rat incisor alveolar bone facing the enamel after root resection." Arch. Histol. Cytol.53(5). 511-522 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ejiri S, Ozawa H.: "Mechanical stress and bone remodeling" Aerospace Science. University Research University, Tokyo.419-425 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nagata M, Ohashi Y, Ozawa H.: Arch. Histol. Cytol.54(3). 267-278 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Irie K, Ozawa H.: "Relationships between tooth eruption, occlusion and alveolar bone resorption : Cytological and cytochemical studies of bone resorption on rat incisor alveolar bone facing the enamel." Arch. Histol. Cytol.53(5). 497-509 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mohri T, Hanada K, Ozawa H.: "Coupling of resorption and formation on bone remodeling sequence in orthodontic tooth movement A histochemical study" J Bone and Mineral Metabolism. 9(2). 57-69 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Amizuka M, Ozawa H.: "Intracellular localization and translocation of 1alpha, 25-dihydroxyvitamin D3 receptor in osteoblasts." Arch. Histol. Cytol.55(1). 77-88 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wu C H, Hara K, Ozawa H.: "97-Enhanced osteoinduction by intramuscular grafting of BMP-beta-TCP compound pellets into murine models." Arch. Histol. Cytol.55(1). 112 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura H, Ozawa H.: "Characteristic localization of carbohydrates in osteoclasts by lectin cytochemistry." Bone. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小澤 英浩: "骨代謝のしくみと骨の細胞群" 診断と治療. 78. 2107-2111 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小澤 英浩: "Bone Cellsの微細構造と機能" 日本臨牀. 12. 2736-2741 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mohri,T.,K.Hanada & H.Ozawa: "Coupling phenomena between bone resportion and formation on bone remodeling in orthodontic tooth movement." J.Bone Mineral Metab.9. 57-69 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 入江 美華,入江 一元,中村 浩彰,江尻 貞一,小澤 英浩: "破骨細胞の分化に関する微細構造学的・オ-トラジオグラフィ-的研究" 日本骨代謝学会誌. 9. 47-53 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Amizuka,N and Ozawa,H: "Intracellular localization and translocation of 1 α,25,ーdihydroxyvitamin D receptor in osteoblasts." Arch.Histol.Cytol.55. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nakamura,H and Ozawa,H.: "Characteristic localization of carbohydrates in osteoclasts by lectin cytochemistry." Bone. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ejiri,S & H.Ozawa: "Aerospace" University Research Center,Nihon University, 615 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小澤 英浩,江尻 貞一,中村 浩彰,入江 一元,入江 美華,網塚 憲生: "骨代謝調節因子ー最近の進歩" 羊土社, 189 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小澤 英浩: "骨芽細胞と破骨細胞の細胞連関。" ホルモンと臨床. 38. 415-421 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小澤 英浩: "骨改造現象における細胞連鎖機構に関する細胞生物学的知見。" 新潟歯学会誌. 20. 1-17 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小澤 英浩: "骨リモデリングの形態学。" 総合臨床. 39. 2539-2550 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Irie,K.and Ozawa,H.: "Relationships between tooth eruption,occlusion and alveolar bone resorption:Cytological and cytochemical studies of bone resorption on rat incisor alveolar bone facing enamel." Arch.Histol.Cytol.53. 497-509 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Irie,K.and Ozawa,H.: "Relationships between tooth eruption,occlusion and alveolar bone resorption:Histochemical and cytological studies of bone remodelling on rat incisor alveolar bone facing enamel after root resection." Archs.Histol.Cytol.53. 511-522 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小澤 英浩: "骨芽細胞と破骨細胞のCellーCell,CellーMatrlx Interactionの形態学的知見。" 日本骨代謝学会誌. 9. 1-8 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 安田 寛基等編,小澤 英浩: "各種硬組織における基質・細胞間、基質・基質間等界面領域の形態学的特徴。「バイオサ-ファクタント」" サイエンスフォ-ラム社, 464 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 尾形 悦朗編,小澤 英浩: "骨改造における骨吸収と骨形成のカップリング現象に関する細胞生物学的知見。「骨粗鬆症研究の進歩」" トプコ出版部, 137 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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