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Gene expression and DNA replication triggered by growth factors and their receptors

Research Project

Project/Area Number 02404086
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

ARAI Ken-ichi  Institute of Medical Science, The University of Tokyo Professor, 医科学研究所, 教授 (00012782)

Co-Investigator(Kenkyū-buntansha) MURAMATSU Masa-aki  Institute of Medical Science, The University of Tokyo Assistant Professor, 医科学研究所, 教務職員 (50230008)
MASAI Hisao  Institute of Medical Science, The University of Tokyo Assistant Professor, 医科学研究所, 助手 (40229349)
NAKAYAMA Naoki  Institute of Medical Science, The University of Tokyo Assistant Professor, 医科学研究所, 助手 (80227967)
YOKOTA Takashi  Institute of Medical Science, The University of Tokyo Associate Professor, 医科学研究所, 助教授 (50134622)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥17,800,000 (Direct Cost: ¥17,800,000)
Fiscal Year 1991: ¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1990: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsDNA replication / G-protein / Signal transduction / primosome / kinase / Cytokine / Transcription factor / Receptor / 増殖因子 / 遺伝子発現 / 細胞周期 / 発現クロ-ニング / 分子生物学
Research Abstract

We intended to clarify the intracellular mechanisms of the cell cycle transition from G1 to S. 1) Control of replication in E. coli : G1 to S phase transition is represented by the initiation of chromosomal DNA replication. The new DNA strand synthesis in E. coli, the most thoroughly studied organism, is initiated by the action of primosome, a protein complex essential for priming DNA templates for DNA polymerase. We demonstrated that two types of primosomes, phiXI74 type (priA-dependent) and ABC type (dnaA-dependent), are functionally interchangeable in the ColEl replication. Furthermore, replication of F, R6K, and Rstl plasmids requires dnaA but not priA, whereas recA-dependent and dnaA-independent stable replicafion in E coli requires priA. We proposed that there are two types of replicons in E. coli, one dependrnt on the ABC primosome, and the other dependent on the phiXI74 type primosome. 2) GI to S transition in yeast : Mating pheromone is a yeast peptide that inhibits the initia … More tion of DNA replicafion, thereby arrests the cell cycle of target cells at Gl phase. We have previously demonstrated that its effect is exerted through a specific membrane receptor and a Gprotein. We further showed that a beta-gamma fusion polypeptide is as active as the natural beta/gamma subunit of the G-protein, so that, as a complex, both subunit are the key elements to introduce the mating pheromone signal. We have been looking for proteins that interact with the core sequence of putative chromosomal replication origins of yeast, which are likely to be the initiation factors for DNA replication. We have found several proteins and purified one of them, 100kD protein, to homogeneity. The CDC7 kinase is implicated to be involved in the initiation of DNA replication in yeast. We have constructed an overproducer of the CDC7 protein, and made antibodies against CDC7. Purification and characterization of these proteins are underway. 3) G1 to S transition in hematopoietic cells : Cytokines are a set of polypeptides that are mainly secreted from activated T cells, and act on various types of cells including hematopoietic cells. They not only support cell's viability and cell cycle progression from Gl to S, but also promote subsequent differentiation. Our research has been focusing on the role of IL-3 and GM-CSF on hematopoietic progenitor cells. Both act on very early progenitor cells to stimulate their proliferation and differentiation. Such similarities in the effects of these factors can be explained by our recent finding that receptors for both cytokines are composed of two polypeptides, alpha and beta, of which beta chain is shared. Analysis of the downstream events initiated upon binding of these cytokines to their receptors are underway. T cells are largely in GO/GI phase during the cell cycle. Upon the activation by antigen, they secrete a number of cytokines including IL-3 and GM-CSF, then they start to initiate DNA replication. We have been analyzing promoter Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (101 results)

All Other

All Publications (101 results)

  • [Publications] Mauxion,F.et.al.: "Comparison of constitutive and inducible transcriptional enhancement mediated by KB-related sequences:modulation of activity in B cells by human T cell leukemia virus type I tax gene" Proceedings of National Academy of Science USA. 88. 2141-2145 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyatake,S.et.al.: "Characterization of the mouse GM-CSF promoter:nuclear factors that interact with an element shared by three lymphokine genes:GM-CSF,IL-4,and IL-5" Molecular and Cellular Biology. 11. 5894-5909 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kitamura,T.et.al.: "Expression cloning of human IL-3 receptor cDNA and reconstitution of high affinity receptors for human IL-3 and GM-CSF" Cell. 66. 1165-1174 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuboi,A.et.al.: "A nuclear factor NFGM2,essential for induction of GM-CSF gene in response to T-cell activation :purification from T cell leukemia line Jurkat cell and relationship to NF-kB" International Immunology. 3. 807-817 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nomura,N.et.al.: "Identification of eleven single strand inifiation sequences(ssi) for priming of DNA replication in the F,R6K,R100 and ColE2 plasmids" Gene. 108. 15-22 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Arai,K.et al.: "Cytokine network and regulation of inflammatory response" Excepta Medica. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishida,J.et.al.: "Definition of GC rich motif as regulatoy sequence of thehuman IL-3 gene:coordinate regulation of the IL-3 gene by CLE2/GC box of the GM-CSF gene in T cell activation" International Immunology. 3. 245-254 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Watanabe,S.et.al.: "Colony stimulating factors and cytokine receptor network" Current Opinion in Biotechnology. 2. 227-237 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kitamura,T.et.al.: "Reconstitution of functional receptors for human granulocyte-macrophage colony stimulating factor: requirement of the b subunit for signaling" Proceedings of National Academy of Science USA. 88. 5082-5086 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Schreurs,J.et.al.: "AIC2A is a component of the purified high affinity mouse IL-3 receptor: temperature-dependent modulation of AIC2A structure" International Immunology. 3. 1231-1242 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ozu,M.et.al.: "Regulation of the IL-3 gene and structure and function of the IL-3 receptor" Advance in Neuroimmunology. 2. 32-52 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wang,H.M.et.al.: "Structure of mouse IL-3 binding protein (AIC2A):Amino acid residues critical for IL-3 binding" Journal of Biological Chemistry. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Garret,K.P.et.al.: "The C-terminus of rat RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function" (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hisatsune,T.et.al.: "A suppressive lymphokine derived from Ts clone 13G2 is IL-10" Lymphokine Research. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyajima A.et.al.: "Cytokine receptors and signal transduction" Annual Review of Immunology. 10. 295-331 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe,E.et.al.: "Eleven-base pair DNA sequence motif unique to human IL-4 gene confers responsiveness to T cell activation signals" Proceedings of National Academy of Science USA. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hatake,K.et.al.: "Human Cytokines" Blackwell Scientific Publications Inc.(Aggarwal,B.B. and Gutterman,J.U.,eds), 135-142(8) (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyatake,S.et.al.: "Molecular Biology of Lymphokines" VCH Publishers,Inc., 35-37(3) (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokota,T.et.al.: "Cytokines and Infectious Disease" Marcel Decker Inc.(Bermudes,L.Z. and Steems,P.,eds), (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji, K., Nakahata, T., Takagi, M., Kobayashi, T., Ishiguro, A., Kikuchi, T., Naganuma, K., Koike, K., Miyajima, A., Arai, K., Akabane, T.: "Effects of interleukin-3 and interleukin-4 on the development of connective tissue-type mast cells : interleukin 3 support their survival and interleukin-4 triggers and supports their proliferation synergistically with interleukin-3" Blood.75. 421-427 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji, K., Nakahata, T., Takagi, M., Kobayashi, T., Ishiguro, A., Kikuchi, T., Naganuma, K., Koike, K., Miyajima, A., Arai, K., Akabane, T.: "Synergistic action of phorbol ester and interleukin 3 in the induction of connective tissue type mast cell proliferation." J. Immunol.144. 678-684 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Schreurs, J., Arai, K., Miyajima, A.: "Evidence for a low affinity interleukin 3 receptor." Growth Factors. 2. 221-234 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Arai, K., Lee, F., Miyajima, A., Miyatake, S., Arai, N., Yokota, T.: "Cytokines : coordinators of immune and inflammatory responses." Ann. Rev. Biochem.59. 783-836 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Nomoto, Nakayama, N., Arai, K., Matsumoto, K.: "Regulation of the yeast pheromone response pathway by G protein subunits." EMBO J.2. 691-696 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsumoto, K., Miyajima, I., Nomoto, S., Nakayama, N., Arai, K.: "Mating pheromone signal transduction in yeast." "Developmental Biology : Molecular and cellular biology of yeast and filametous funji"UCLA Symposia. Wiley-Liss Inc. 289-306 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masai, H., Nomura, N., Kubota, Y. and Arai, K.: "Role of phiX174 type primosome- and G4 type primase-dependent primings in initiation of lagging and leading strand synthesis of DNA replication." J. Biol. Chem.266. 15124-15133 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyatake, S., Yokota, T., Miyajima, A., Kitamura, T., Arai, N. and Arai, K.: "Molecular biology of lymphokines." "Immunology and Biotechnology in Biomedical Research Vol II Current Concepts in Immunology". Verlag chemie International.35-77 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Itoh, N., Yonehara, S., Schreurs, J., Gorman, M. D., Maruyama, K., Ishii, A., Yahara, I., Arai, K. and Miyajima, A.: "Cloning of an interleukin-3 receptor gene : a member of a distinct receptor gene family." Science. 247. 324-327 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yonehara, S., Ishii, A., Yonehara, M., Koyasu, S., Miyajima, A., Schreurs, J., Arai, K. and Yahara, I.: "Identification of a cell surface 105 Kd protein which binds interleukin 3" Int. Immunol.2. 143-150 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyajima, A., Itoh, N., Schreurs, J., Gorman, D. M., Maruyama, K., Yonehara, S., Yahara, I. and Arai, K.: "Cloning of a cDNA encoding a murine interleukin 3 receptor." "Molecular and Cellular Biology of Cytokines". Wiley-Liss Inc. 149-154 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Schreurs, J., Sugimoto, K., Wang, H. -M., Nishida, J., Heike, T., Miyatake, S., Abe, E., Ninomiya-Tsuji, J., Muramatsu, M., Ito, N., Gorman, D., Maruyama, K., Xie, G. -X., Kitamura, T., Hatake, K., Hayashida, K., de Waal Malefyt, R., Shlomai, J., Tto, N., Yokota, T., Arai, N., Miyajima, A. and Arai, K.: "Lymphokine receptor signal transduction and regulation of lymphokine genes." EOS J Immunol. Immunopharm.(1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokota, Y., Arai, N., Arai, K. and Zlotnik, A.: "Interleukin-4." "Handbook of Experimental Pharmacology. ". Springer-Verlag. 577-607 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishida, J., Arai, K. and Yokota, T.: "Regulation of B cell response by cytokines, IL-4, IL-5, IL-l : Biological and Molecular aspects." Ann. Natl. Health Inst. Rome. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Maruyama, K., Miyajima, A., Yokota, T., Arai, K. and Goldstein, A.: "Chimeric opioid peptides : tools for identifying opioid receptor types. Xie, G. -X." Proc. Natl. Acad. Sci, USA. 87. 3180-3184 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masai, H., Nomura, N. and Arai, K.: "The ABC primosome : a novel priming system employing dnaA, dnaB, dnaC and primase on a hairpin containing a dnaA box sequences." J. Biol. Chem.265. 15134-15144 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugimoto, K., Miyatake, S., Arai, K., and Arai, N.: "Inducible and non-inducible factors cooperatively activate the GM-CSF promoter by interacting with two adjacent DNA motifs." Int. Immunol.2. 787-794 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Arai, N., Miyatake, S., Heike, T., Takebe, Y., Sugimoto, K., Arai, K.: "Activation of lymphokine genes and viral promoters by BPV E2 and HTLV-I p40^<tax> proteins" "Papillomaviruses". Wiley-Liss. 523-532 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Gorman, D. M., Itoh, N., Kitamura, T., Schreurs, J., Yonehara, S., Yahara, I., Arai, K. and Miyajima, A.: "Cloning and expression of a novel IL-3 receptor-like gene : identification of another member of the cytokine receptor gene family." Proc. Natl. Acad. Sci. USA. 87. 5459-5463 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashida, K., Kitamura, T., Gorman, D. M., Arai, K., Yokota, T. and Miyajima, A.: "Molecular cloning of a second subunit of the receptor for human granlocyte-macrophage colony stimulating factor (GM-CSF) : Reconstitution of a high-affinity GM-CSF receptor." Proc. Natl. Acad. Sci. USA. 87. 9655-9659 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe, E., Yoshida, Arai, K., Arai, N.: "Mapping of a human Il-4 gene regulatory region that mediates the response to T cell activation signals, HYLV-I p40^<tax> and BPVE2" "Hematopoiesis". Allan R. Liss Inc.91-95 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishida, J., Yoshida, M., Arai, K., and Yokota, T.: "Definition of GC rich motif as regulatory sequence of the human IL-3 gene : Coordinate regulation of the IL-3 gene by CLE2/GC box of the GM-CSF gene in T cell activation" Int. Immunol.3. 245-254 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Watanabe, S., Nakayama, N., Yokota, T., Miyajima, A. and Arai, K.: "Colony stimulating factors and cytokine receptor network" Current Opinion in Biotechnol.2. 227-237 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kitamura, T., Hayashida, K., Sakamaki, K., Yokota, T., Arai, K. and Miyajima, A.: "Reconstitution of functional receptors for human granulocyte-macrophage colony stimulating factor : Requirement of the b subunit for signaling" Proc. Natl. Acad. Sci. USA. 88. 5082-5086 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Arai, K., Nakayama, A. and Yokota, T.: "Cytokine network and regulation of Inflammatory Response" Excepta Medica.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hatake, K., Arai, K. and Yokota, T.: "Interleukin-5" "Human Cytokines". Blackwell Scientific Publications Inc. 135-142 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mauxion, F., Jamieson, C., Yoshida, M., Arai, K. and Sen, R.: "Comparison of constitutive and inducible transcriptional enhancement mediated by KB-related sequences : modulation of activity in B cells by human T cell leukemia virus type I tax gene." Proc. Natl. Acad. Sci. USA. 88. 2141-2145 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyatake, S., Shlomai, J., Arai, K. and Arai, N.: "Characterization of the mouse GM-CSF promoter : Nuclear Factors That Interact with an Element Shared by Three Lymphokine Genes : GM-CSF, IL-4 and IL-5" Mol. Cell. biol.11. 5894-5909 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyatake, S., Yokota, T., Miyajima, A., Kitamura, T., Arai, N. and Arai, K.: "Molecular Biology of Lymphokines." VCH Publishers, Inc.35-37 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katamura, T., Sato, N., Arai, K. and Miyajima, A.: "Expression cloning of human IL-3 receptor cDNA and reconstitution of high affinity receptors for human IL-3 and GM-CSF" Cell. 66. 1165-1174 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuboi, A., Sugimoto, K., Yodoi, J., Miyatake, S., Arai, K., and Arai, N.: "A nuclear factor NFGM2, essential for induction of GM-CSF gene in response to T-cell activation ; purification from human T-cell leukemia line Jurkat cells and relationship to NF-KB." Int. Immunol.3. 807-817 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nomura, N., Masai, Hisao., Inuzuka, M., Miyazaki, C., Ohtsubo, E., Itoh, T., Sasamoto, S., Matsui, M., Ishizaki, R. and Arai, K.: "Identification of eleven single strand initiation sequences (ssi) for priming of DNA replication in the F, R6K, R100 and ColE2 plasmids" Gene. 108. 15-22 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyajima, A., Kitamura, T., Harada, N., Yokota, T. and Arai, K.: "Cytokine receptors and signal transduction" Ann. REv. Immunol. 10. 295-331 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Arai, K. and Watanabe, S.: "New era of biochemistry : cytokine signal network and its implication for biomedical research" "Medicinal Chemistry for 21st Century". IUPAC in press. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Abe, E., Matsuda, I., de Waal Malefijit, R., Arai, K., and Arai, N.: "Eleven-base pair DNA sequence motif unique to human IL-4 gene confers responsiveness to T cell activation signals." Proc. Natl. Acad. Sci, USA. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Schreurs, J., Hung, P., May, W. S., Arai, K., and Miyajima, A.: "AIC2A is a component of the purified high affinity mouse IL-3 receptor : temperature-dependent modulation of AIC2A structure." Int. Immunol.3. 1231-1242 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yokota, T., Hatake, K., Ave, E., Harada, N., Miyajima, A., Arai, N. and Arai, K.: "Interleukin 4" "Cytokines and Infectious Disease". Marcel Decker. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Watanabe, S., Yssel, H., Yokota, T., Arai, N., Miyajima, A. and Arai, K.: "Molecular biology of T cell derived cytokines and cytokine receptor network" "XIV International Congress of Allergology and Clinical Immunology". (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ozu, M., Arai, K. and Yokota, T.: "Regulation of the IL-3 gene and structure and function of the IL-3 receptor" Advance in Neuroimmunol.2. 32-52 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hisatsune, T., Minai, Y., Nishisima, K., Enomoto, A., Moore, K., Yokota, T., Arai, K. and Kaminogawa, S.: "A Suppressive lymphokine derived from Ts clone 13G2 is IL-10" Lymphokine Res.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Wang, H. M., Ogorochi, T., Arai, K., and Miyajima, A.: "Structure of mouse IL-3 binding protein (AIC2A) : Amino acid residues critical for IL-3 binding." J. Biol. Chem.267. 979-983 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Arai, N., and Arai, K.: "IL-4 gene : regulation of expression." "IL-4 : structure and function". Marcel Decker. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Garret, K. P., Serizawa, H., Bradsher, J. N., Hansley, J. P., Tsuboi, A., Arai, N., Yokota, T., Arai, K., Conaway, R. C., Conaway, J. W.: "The C-terminus of rat RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function" (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyajima, A., Hayashida, K., Itoh, N., Schreurs, J., Gorman, D. M., Yonehara, S., Yahara, I., Takagi, K., Yokota, T., Arai, K. and Kitamura, T.: "Molecular structure of the receptors for GM-CSF, IL-3 and IL-5" "Second International Symposium on Molecular Biology of Hematopoiesis". (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mauxion,F.et.al.: "Comparison of constitutive and inducible transcriptional enhancement mediated by KBーrelated sequences:modulation of activity in B cells by human T cell leukemia virus type I tax gene" Proceedings of National Academy of Science USA. 88. 2141-2145 (1991)

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      1991 Annual Research Report
  • [Publications] Miyatake,S.et.al.: "Characterization of the mouse GMーCSF promoter:nuclear factors that interact with an element shared by three lymphokine genes:GMーCSF,ILー4,and ILー5" Molecular and Cellular Biology. 11. 5894-5909 (1991)

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      1991 Annual Research Report
  • [Publications] Kitamura,T.et.al.: "Exprssion cloning of human ILー3 receptor cDNA and reconstitution of high affinity receptors for human ILー3 and GMーCSF" Cell. 66. 1165-1174 (1991)

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      1991 Annual Research Report
  • [Publications] Tsuboi,A.et.al.: "A nuclear factor NFGM2,essential for induction of GMーCSF gene in response to Tーcell activation:purification from T cell leukemia line Jurkat cell and relationship to NFーKB" International Immunology. 3. 807-817 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nomura,N.et.al.: "Identification of eleven single strand initiation sequences(ssi) for priming of DNA replication in the F,R6K,R100 and ColE2 plasmids" Gene. 108. 15-22 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Arai,K.et.al.: "Cytokine network and regulation of inflammatory response" Excepta Medica. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishida,J.et.al.: "Definition of GC rich motif as regulatoy sequence of thehuman ILー3 gene:coordinate regulation of the ILー3 gene by CLE2/GC box of the GMーCSF gene in T cell activation" International Immunology. 3. 245-254 (1991)

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      1991 Annual Research Report
  • [Publications] Watanabe,S.et.al.: "Colony stmulating factors and cytokine receptor network" Curren Opinion in Biotechnology. 2. 227-237 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kitamura,T.et.al.: "Reconstitution of functional receptors for human granulocyteーmacrophage colony stimulating factor:requirement of the b subunit for signaling" Proceedings of National Academy of Science USA. 88. 5082-5086 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Schreurs,J.et.al.: "AIC2A is a component of the purified high affinity mouse ILー3 receptor:temperatureーdependent modulation of AIC2A structure" International Immunology. 3. 1231-1242 (1991)

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      1991 Annual Research Report
  • [Publications] Ozu,M.et.al.: "Regulation of the ILー3 gene and structure and function of the ILー3 receptor" Advance in Neuroimmunology. 2. 32-52 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Wang,H.M.et.al.: "Structure of mouse ILー3 binding protein (AIC2A) Amino acid residues critical for ILー3 binding" Journal of Biological Chemistry. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Garret,K.P.et.al.: "The Cーterminus of rat RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function" submitted. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hisatsune,T.et.al.: "A suppressive lymphokine derived from Ts clone 13G2 is ILー10" Lymphokine Research. (1992)

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      1991 Annual Research Report
  • [Publications] Miyajima A.et.al.: "Cytokine receptors and signal transduction" Annual Review of Immunology. 10. 295-331 (1992)

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      1991 Annual Research Report
  • [Publications] Abe,E.et.al.: "Elevenーbase pair DNA sequence motif unique to human ILー4 gene confers responsiveness to T cell activation signals" Proceedings of National Academy of Science USA. (1992)

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      1991 Annual Research Report
  • [Publications] Hatake,K.et.al.: "Human Cytokines" Blackwell Scientific Publications Inc.(Aggarwal,B.B.and Gutterman,J.U.,eds), 135-142(8) (1991)

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      1991 Annual Research Report
  • [Publications] Miyatake,S.et.al.: "Moleculoar Biology of Lymphokines" VCH Publishers,Inc., 35-37(3) (1991)

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      1991 Annual Research Report
  • [Publications] Yokota,T.et.al.: "Cytokines and infectious Disease" Marcel Decker Inc.(Bermudes,L.Z.and Steems,P.,eds), (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishida,J.: "Deinition of GC rich motif as regulatory sequence of the human ILー3 gene:Coordinate regulation of the ILー3 gene by CLE2/GC box of the GMーCSF gene in T cell activation." Int Immunol.(1991)

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      1990 Annual Research Report
  • [Publications] Hayashida,K.: "Molecular cloning of a second subunit of the human GMーCSF receptor:Resonsitution of a high affinity GMーCSF receptor by coーexpression of the low affinity GMーCSF receptor and an ILー3 receptorーlike molecule." Proc Natl Acad Sci U S A.87. 9655-9659 (1990)

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      1990 Annual Research Report
  • [Publications] Itoh,N.: "Molecular cloning of a cDNA encoding the murine interleukinー3 receptor reveals a consensus motif for a new cytokine receptor gene family." Science. 247. 324-327 (1990)

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      1990 Annual Research Report
  • [Publications] Gorman,D.M.: "Cloning and expression of a gene encoding an interleukin 3 receptorーlike protein:Identification of another member of the cytokine receptor gene family." Proc Natl Acad Sci U S A.87. 5459-5463 (1990)

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  • [Publications] Sugimoto,K.: "Inducible and nonーinducible factors coーoperatively active the GMーCSF promoter by interacting with two adjacent DNA motifs." Int Immunol.2. 787-794 (1990)

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      1990 Annual Research Report
  • [Publications] Arai,K.: "Cytokines;Cordinators of immune and inflammatory responses." Ann Rev Biochem. 59. 783-836 (1990)

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      1990 Annual Research Report
  • [Publications] Tsuji,K.: "Effects of interleukinー3 and interleukinー4 on the development of connective tissueーtype mast cells:interleukinー3 supports their survival and interleukinー4 triggers and supports their proliferation synergistically with interleukinー3." Blood. 75. 421-427 (1990)

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      1990 Annual Research Report
  • [Publications] Tsuji,K.: "Synergistic action of phorbol ester and interleukin 3 in the induction of connective tissue type mast cell proliferation." J Immunol. 144. 678-684 (1990)

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      1990 Annual Research Report
  • [Publications] Xie,G.: "Chimeric opioid peptides:tools for identifying opioid receptor types." Proc Natl Acad Sci USA. 87. 3180-3184 (1990)

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      1990 Annual Research Report
  • [Publications] Schreurs,J.: "Evidence for a low affinity interleukin 3 receptor." Growth Factors. 2. 221-234 (1990)

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      1990 Annual Research Report
  • [Publications] Watanabe,S.: "Colony stimulating factors and cytokine receptor network." Current opinion in Biotechnology. (1991)

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      1990 Annual Research Report
  • [Publications] Masai,H.: "Roles of φX174 type primosome and G4 type primaseーdependent primings in initiation of lagging and leading strand synthesis of DNA replication" The Journal of Biological Chemistry. 265. 15124-15133 (1990)

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      1990 Annual Research Report
  • [Publications] Masai,H.: "The ABC primosome:A novel priming system employing dnaA,dnaB,dnaC and primase on a hairpin containing a dnaA box sequence" The Journal of Biological Chemistry. 265. 15134-15144 (1990)

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      1990 Annual Research Report
  • [Publications] Lee,E.H.: "The priA gene encoding the primosomal replicativen' protein of Escherichia coli." Proc Natl Acad Sci USA. 87. 4620-4624 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nomura,N.: "Identification of eleven singleーstranded sequences (ssi) for priming of DNA replication in the F,R6K,R100 and ColE2 plasmids" Gene. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nomoto,S.: "Regulation of the yeast pheromone response pathway by G protein subunits." EMBO J. 9. 691-696 (1990)

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      1990 Annual Research Report
  • [Publications] Matsumoto,K.: "Mating pheromone signal transduction in yeast." “Molecular and cellular biology of yeast and filamentous funji"UCLA Symposia. (1990)

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      1990 Annual Research Report
  • [Publications] Yokota,T.: "Handbook of Experimental Pharmacology,Vol.95/I Peptide Growth.Factors and Their Receptors I." SpringerーVerlag, 31 (1990)

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      1990 Annual Research Report
  • [Publications] Miyatake,S.: "Molecular biology of cytokines." Verlag Chemie International, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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