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DNA-PROTEIN INTERACTION IN DNA REPLICATION

Research Project

Project/Area Number 02404088
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionNATIONAL INSTITUTE OF GENETICS

Principal Investigator

HORIUCHI Kensuke  National Institute of Genetics, Dept.Cell Genetics, Professor, 細胞遺伝研究系, 教授 (70219210)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Yukinobu  Toho University, Faculty of Science, Professor, 理学部, 教授 (30029699)
NISHIMURA Akiko  National Institute of Genetics, Genet.Stock Res.Center, Associate Professor, 微生物保存研究室, 助教授 (20142002)
HIGASHITANI Atsushi  National Institute of Genetics, Dept.Cell Genetics, Assistant Professor, 細胞遺伝研究系, 助手 (40212162)
HARA Hiroshi  National Institute of Genetics, Dept.Cell Genetics, Assistant Professor, 細胞遺伝研究系, 助手 (00173071)
YASUDA Seiichi  National Institute of Genetics, Dept.Cell Genetics, Associate Professor, 細胞遺伝研究系, 助教授 (90037250)
Project Period (FY) 1990 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥29,000,000 (Direct Cost: ¥29,000,000)
Fiscal Year 1993: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1992: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1991: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1990: ¥12,400,000 (Direct Cost: ¥12,400,000)
Keywordsreplication origin / filamentous phage / DNA bending / superhelix / rolling circle / Escherichia coli / SOS / cell division / 大腸菌 / DNA結合蛋白 / 超〓旋 / 熱ショック蛋白 / シャペロン / RNAポリメラーゼ / IHF蛋白 / 繊維状ファ-ジ / 蛋白リン酸化 / 浸透圧 / 細胞分裂 / fts遺伝子群 / 染色体地図
Research Abstract

1) We studied protein-DNA interactions in initiation of DNA replication of filamentous bacteriophages of E.coli. In relation to replication of the leading (plus) strand, we found that (a) the initiation frequency is a function of negative superhelical density of template DNA, (b) the initiator protein (gpII) specifically binds and bends DNA in a stepwise fashion, (c) this leads to melting of duplex in the nicking region, (d) the melting is a prerequisite for the initiation, and (e) negative superhelicity is required for the melting to occur. The binding of gpII to DNA is co-operative and involves protein-protein interactions. These results indicate that the initiation of replication is preceded by an ordered series of protein-induced DNA conformational changes. The replication enhancer region contains a strong binding site for the host IHF protein, which bends DNA.Our results indicate that interaction between two regions flanking the IHF site is essential for the function of the enhancer. Replication of the lagging (minus) strand is initiated by a RNA primer that is synthesized by the host RNA polymerase. We studied the interaction between the enzyme and the origin DNA.The results suggested that there is certain similarity in recongnition mechanisms between initiation of transcription and primer synthesis. We also found that mutant phage that are defective in the origin for lagging strand synthesis induce the SOS response in the infected cells, and demonstrated that single-stranded DNA per se is the SOS signal in vivo. 2) We studied how a dnaK mutation in E.coli depresses initiation of chromosomal replication, and found that DnaK stabilizes the initiator protein DnaA in vitro. 3) We completed mapping of a large number of fts genes, which are involved in cell division. 4) We identified the natural promoter for penicillin-binding protein 3. This promoter appears to serve for transcription of a large operon that includes many genes involved in cell division.

Report

(5 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • 1990 Annual Research Report

Research Products

(89 results)

All Other

All Publications (89 results)

  • [Publications] Higashitani,A.et al.: "Multiple DNA conformational changes induced by an initiator protein preceed the nicking reaction in a rolling circle replication origin." J.Mol.Biol.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani,N.: "Nucleotide sequence of the primer RNA for DNA replication of filamentous bacteriophages." J.Virol.67. 2175-2181 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani,A.: "Osmoregulation of the fatty acid receptor gene fadL in Esherichia coli." Mol.Gen.Genet.240. 339-347 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda,Y.: "Isolation of temperature-sensitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid." Mol.Gen.Genet.238. 169-176 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda,Y.: "chpA and chpB,Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." J.Bacteriol.175. 6850-6856 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani,A.: "A single amino acid substitution reduces the superhelicity requirement of a replication initiator protein." Nucleic Acids Res.20. 2685-2691 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani,N.: "SOS induction in Escherichia coli by infection with mutant filamentous phage that are defective in initiation of the complementary strand DNA synthesis." J.Bacteriol.174. 1612-1618 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Specthrie,L.: "Construction of a microphage variant of filamentous bacteriophage." J.Mol.Biol.228. 720-724 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsuchimoto,S.: "The stable maintenance system pem of plasmid R100:Degradation of PemI protein may allow PemK protein to inhibit cell growth." J.Bacteriol.174. 4205-4211 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishimura,A.: "Correlation of a subset of the pLC-plasmids to the physical map of Escherichia coli K12." Microbiol.Rev.56. 137-151 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Russell,D.W.: "The mutH gene regulates the replication and methylation of the pMB1 origin." J.Bacteriol.173. 3209-3214 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hara,H.: "Cloning,mapping,and characterization of the Escherichia coli prc gene,which is involved in C-terminal processing of penicillin-binding protein 3." J.Bacteriol.173. 4799-4813 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishimura,A.: "A rapid and highly efficient method for preparation of competent Escherichia coli cells." Nucleic Acids Res.18. 6169 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato,J.: "New topoisomerase essential for chromosome segregation in E.coli." Cell. 63. 393-404 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujisaki,S.: "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli." J.Biochem.108. 995-1000 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Greenstein,D.: "Replication enhancer-independent mutation increases the co-operativity with which an initiator protein binds its origin." J.Mol.Biol.211. 91-101 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 堀内賢介: "繊維状ファージの複製機構と蛋白質展示ベクターとしての応用." 蛋白質核酸酵素. 37. 2589-2598 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 堀内賢介: "繊維状ファージのDNA複製開始域." 遺伝学雑誌. 65. 225-241 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hitashitani, A.et al.: "Multiple DNA conformational changes induced by an initiator protein preceed the nicking reaction in a rolling circle replication origin." J.Mol.Biol.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani, N.et al.: "Nucleotide sequence of the primer RNA for DNA replication of filamentous bacteriophages." J.Virol.67. 2175-2181 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashinati, A.et al.: "Osmoregulation of the fatty acid receptor gene fadL in Esherichia coli." Mol.Gen.Genet.240. 339-347 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda, Y.et al.: "Isolation of temperature-sensitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid." Mol.Gen.Genet.238. 169-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda, Y.et al.: "chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." J.Bacteriol.175. 6850-6856 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani, A.et al.: "A single amino acid substitution reduces the superhelicity requirement of a replication initiator protein." Nucleic Acids Res.20. 2685-2691 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashitani, N.et al.: "SOS induction in Escherichia coli by infection with mutant filamentous phage that are defective in initiation of the complementary strad DNA synthesis." J.Bacteriol.174. 1612-1618 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Specthrie, L.et al.: "Construction of a microphage variant of filamentous bacteriophage." J.Mol.Biol.228. 720-724 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsuchimoto, S.et al.: "The stable maintenance system pem of plasmid R100 : Degradation of PemI protein may allow PemK protein to inhibit cell growth." J.Bacteriol.174. 4205-4211 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishimura, A.et al.: "Correlation of a subset of the pLC-plasmids to the physical map of Escherichia coli K12." Microbiol.Rev.56. 137-151 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Russell, D.W.& Horiuchi, K.: "The mutH gene regulates the replication and methylation of the pMB1 origin." J.Bacteriol.173. 3209-3214 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hara, H.et al.: "Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3." J.Bacteriol.173. 4799-4813 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishimura, A.et al.: "Mapping of a whole set of cell division genes in Escherichia coli K-12." Control of cell growth and division (eds. A.Ishihama and H.Yoshikawa). pp. 205-223. Japan Sci.Soc.Press, Tokyo/Springer-Verlag, Berlin. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishimura, A.et al.: "A rapid and highly efficient method for preparation of competent Escherichia coli cells." Nucleic Acids Res.18. 6169 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato, J.et al.: "New topoisomerase essential for chromosome segregation in E.coli." Cell. 63. 393-404 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujisaki, S.et al.: "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli." J.Biochem.108. 995-1000 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Greenstein, D.& Horiuchi, K.: "Replication enhancer-independent mutation increases the co-operativity with which an initiator protein binds its origin." J.Mol.Biol.211. 91-101 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Horiuchi, K.& Higashitani, A.: "Filamentous bacteriophage of E.coli. (in Japanese)" Protein, Nucleic Acid and Enzyme. 37. 2589-2598 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Horiuchi, K.: "Origin of DNA replication of filamentous coliphages. (in Japanese)" Jpn.J.Genet.65. 225-241 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Higashitani.: "Multiple DNA conformational changes induced by an initiator protein precede the nicking reaction in a rolling circle replication origin." J.Mol.Biol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Higashitani: "Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli." Mol.Gen.Genet.240. 339-347 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Higashitani: "Nucleotide sequence of the primer RNA for DNA replication of filamentous bacteriophages." J.Virol.67. 2175-2181 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Masuda: "Isolation of temperature-sensitive aminoacy1-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid." Mol.Gen.Genet.238. 169-176 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Masuda: "chpA and chpB,Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." J.Bacteriol.175. 6850-6856 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Higashitani: "Recognition of DNA replication origin of bacteriophage f1 by RNA polymerase." Annual Report of National Institute of Genetics. 43. 34-35 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Asano: "Requirement of the correct phasing within the replication enhancer of phage f1." Annual Report of National Institute of Genetics. 43. 35-36 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Higashitani: "Conformational changes induced in a rolling circle replication origin by initiator protein." Annual Report of National Institute of Genetics. 43. 36-38 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Yasuda: "Stabilization of replication initiator protein DnaA OF Escherichia coli by DnaK chaperone." Annual Report of National Institute of Genetics. 43. 38-38 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Hara: "A1.9-kb upstream sequence is required for expression of the ftsI gene coding for penicillin-binding protein 3of Escherichia coli." Annual Report of National Institute of Genetics. 43. 39-40 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Yamamoto: "A chain-forming strain,PM61,of Escherichia coli contains,in addition to the envC mutation,an IS4 insertion in the prc gene which causes accumulation of the precursor form of penicillin-binding protein3." Annual Report of National Institute of Genetics. 43. 40-41 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Higashitani: "Osmoregulation of the fatty acid receptor gene fadL through OmpR protein in Escherichia coli." Annual Report of National Institute of Genetics.43. 41-42 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Nishimura: "Molecular mechanism in determination of timing of cell division in Escherichia coli K-12(I)." Annual Report of National Institute of Genetics. 43. 42-42 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Ukai: "Molecular mechansim in determination of timing of cell division in Escherichia coli K-12(II)." Annual Report of National Institute of Genetics. 43. 43-43 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Higashitani,N.: "Nucleotide sequence of primer RNA for DNA replication of filamentous bacteriophages" J.Virol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Higashitani,A.: "A single amino acid substitution reduces the superhelicity requirement of a replication initiator prltein" Nucleic Acids Res.20. 2685-2691 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Specthrie,L.: "Construdtion of a microphage variant of filamentous bacteriophage" J.Mol.Biol.228. 720-724 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Higashitani,N.: "SOS induction in Escherichia coli by infection with mutant filamentous phage that are defective in initiation of the complementary strand DNA synthesis" J.Bacteriol.174. 1612-1618 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishimura,A.: "Correlation of a subset of the pLC-plasmids to the physical map of Escherichia coli K-12" Microbiol.Rev.56. 137-151 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masuda,Y.: "Isolation of temperature senaitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemk,plasmid" Mol.Gen.Genet.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsuchimoto,S.: "The stable maintenance system pem of plasmid R100:Degradation of PemI protein may allow Pemk protein to inhibit cell growth" J.Bactiriol.174. 4205-4211 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Higashitani,N.: "Sequencing analysis of primer RNA for minus strand synthesis of bacteriophage fl" Annual Report of National Institute of Genetics. 42. 38-39 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Higashitani,A.: "A single amino acid substitution reduces the superhelicity requirement of a replication initiator protein" Annual Report of National Institute of Genetics. 42. 39-40 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasuda,S.: "Dnak protein is involved in replication of Escherichia coli chromosome by protecting the initiation protein DnaA from heat inactivation" Annual Report of National Institute of Genetics. 42. 41-41 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Higashitani,A.: "In vitro protein phosphorylation in Escherichia coli cell cycle mutants" Annual Report of National Institute of Genetics. 42. 41-42 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Higashitani,A.: "Osmotic regulation of uptake of long-chain fatty acids in Escherichia coli:In vitro interaction of OmpR protein with a fatty acid receptor gene fadL" Annual Report of National Institute of Genetics. 42. 43-44 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishimura,A.: "Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K 12" Annual Report of National of Genetics. 42. 44-44 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Russell,D.W.: "The mutH gene regulates the replication and methylation of the pMB1 origin." J.Bacteriol.173. 3209-3214 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hara,H.: "Cloning,mapping,and characterization of the Escherichia coli prc gene,which is involved in Cーterminal processing of penicillinーbinding protein 3." J.Bacteriol.173. 4799-4813 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishimura,A.: "Correlation of a subset of the pLCーplasmids to the physical map of Escherichia coli Kー12." MiCrobiol.Rev.56. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Higashitani,N.: "SOS induction in Escherichia coli by infection with mutant filamentous phage that are defective in initiation of the complementary strand DNA synthesis." J.Bacteriol.174. 1612-1618 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Higashitani,A.: "A single amino acid substitution reduces the superhelicity requirement of a replication initiator protein."

    • Related Report
      1991 Annual Research Report
  • [Publications] Higashitani,N.: "Mutational analysis of the minus strand origin of bacteriophage fl." Annual Report of National Institute of Genetics. 41. 31-32 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Higashitani,N.: "SOS induction in E.coli by infection with mutant filamentous phages that are defective in complementary strand synthesis." Annual Report of National Institute of Genetics. 41. 33-34 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Higashitani,A.: "Replication of filamentous phage fl: Relationship between DNA topology and the nicking activity of the initiator protein(gp II)." Annual Report of National Institute of Genetics. 41. 34-36 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujisaki,S.: "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli." Annual Report of National Institute of Genetics. 41. 36-37 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 堀内 賢介: "繊維状ファ-ジのDNA複製開始域" 遺伝学雑誌. 65. 225-241 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishimura,Akiko: "A rapid and highly efficient method for preparation of competent Escherichia coli cells." Nucleic Acids Res.18. 6169-6169 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kato,Junーichi: "New topoisomerase essential for chromosome segregation in E.coli." Cell. 63. 393-404 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujisaki,Shingo: "Cloning and nucleotide sequence of the ispA gene responsible for fornesyl diphosphate synthase activity in Escherichia coli." J.Biochem.108. 995-1000 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Greenstein,David: "Replicationーindependent mutation increases the coーoperativity with which an initiator protein binds its origin." J.Mol.Biol.211. 91-101 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Higashitani,Atsushi: "Replication of filamentous phage fl:Enhancerーindependent mutation of an initiator protein (gpII)." Annual Report of National Institute of Genetics. 40. 43-44 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Higashitani,Atsushi: "Multicopy suppression in E.coli:Cloning of sufB,a dosagedependent suppressor of a thermosensitive allーdivision mutation,ftsB." Annual Report of National Institute of Genetics. 40. 44-46 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kato,Junーichi: "A novel topoisomerase essential for the E.coli chromosome partition." Annual Report of National Institute of Genetics. 40. 46-48 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hara,Hiroshi: "Identificatior and cellular localization of the product of prc,a gene involved in the Cーterminal processing of penicillinーbinding protein 3 of Escherichia coli." Annual Report of National Institute of Genetics. 40. 49-51 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamamoto,Yoshihiro: "Nucleotide sequence of the prc gene of Escherichia coli." Annual Report of National Institute of Genetics. 40. 51-52 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujisaki,Shingo: "A mutant producing temperatureーsensitive fornesyl diphosphate synthase of Escherichia coli." Annual Report of National Institute of Genetics. 40. 52-53 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishimura,Akiko: "Mapping a whole set of cell division genes in Escherichia coli kー12." Annual Report of National Institute of Genetics. 40. 53-54 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishimura,Akiko: "Correlation of pLCーplasmids to the physical map of Escherichia coli Kー12."

    • Related Report
      1990 Annual Research Report
  • [Publications] Hara,Hiroshi: "Cloning,mapping and characterization of the Escherichia coli prc gene involved in Cーterminal processing of penicillinーbinding protein 3."

    • Related Report
      1990 Annual Research Report
  • [Publications] Higashitani,Atsushi: "Replication enhancerーindependent mutations reduce superhelicity requirement of the initiator protein of bacteriophage f1."

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishimura,Akiko: "Control of Cell Growth and Division (H.Yoshikawa and A.Ishihama,eds.)の内、Mapping of a whole set of cell division genes in Escherichia coli Kー12." Academic Press, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-03-31   Modified: 2016-04-21  

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