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Membrane protein transfer from intact cell to liposome

Research Project

Project/Area Number 02453099
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子物性・高分子材料(含機械材料)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SUNAMOTO Junzo  Kyoto University, Polymer Chemistry, Professor, 工学部, 教授 (80037811)

Co-Investigator(Kenkyū-buntansha) SATO Toshinori  Kyoto University, Polymer Chemistry, Assistant Professor, 工学部, 助手 (00162454)
AKIYOSHI Kazunari  Kyoto University, Polymer Chemistry, Assistant Professor, 工学部, 助手 (90201285)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1991: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1990: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordsmembrane protein / liposome / transfer / liposomal vaccine / cancer cell / boundary lipid / erythrocyte / platelet / 膜タンパク再構成リポソ-ム / 人工ワクチン
Research Abstract

Perfect extraction of intrinsic membrane proteins from intact cells and reconstitution of them without any denaturation and deactivation are basic requirement in investigation of the function of membrane proteins. For this purpose, artificial cell liposome seem to be a desirable and promising tool in both basic investigation and application. In order to improve the reconstitution efficiency of membrane proteins into liposomes, we have developed a novel artificial boundary lipid, 1,2-dimyristoylamido-1,2-deoxy- phosphatidylcholine (DDPC), of which alkyl chains are linked by an amide bond. When DDPC-containing liposomes were coincubated with intact cells such as erythrocyte, platelet and tumor cell, several membrane proteins were able to be effectively transferred from the intact cell to the liposome. Using this technique, furthermore, we succeeded in development of liposomal vaccine against BALB RVD leukemia cell for tumor surface antigenic proteins (TSAP) transferred from the tumor cells to liposomes.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] J.Sunamoto: "Synthesis and Characterization of 1,2-Dimyristoylamide-1,2-deoxyphosphatidylcholine as a Boundary Lipid Model." Biochim.Biophys.Acta. 1024. 209-219 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Sunamoto: "Deuterium Nuclear Magnetic Resonance Studies on the Interaction of Glycophorin with 1,2-Dimyristoylamido-1,2-deoxyphosphatidylcholine" Biochim.Biophys.Acta. 1024. 220-226 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Sunamoto: "Effective Transfer of Membrane Proteins from Human Erythrocytes to Artificial Boundary Lipid Containing Liposomes" Chem.Lett.1990. 1249-1252 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Sunamoto: "Structural Stability of Lecithin Liposomes as Improved by Adding an Artificial Boundary Lipid." Chem.Lett.1990. 2141-2144 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Sunamoto: "Effective Transfer of Membrane Proteins from Intact Cells to Liposomes and Preparation of Liposomal Vaccines" Ann.N.Y.Acad.Sci.(Proceedings of the 10th Enzyme Engineering Conference). 613. 116-127 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R.Shibata: "Induction of in vitro and in vivo Anti-Tumor Responses by Sensitization of Mice with Liposomes Containing a Crude Butanol Extract of Leukemia Cells and Transferred Intermembranously with Cell Surface Proteins" Int.J.Cancer. 48. 434-442 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Sunamoto: "Direct Transfer of Tumor Surface Antigen-Presenting Protein(TSAP)from Tumor Cell to Liposome for Making Liposomal Vaccine" J.Controlled Release.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Sunamoto: "Synthesis and Characterization of 1,2-Dimyristoylamido-1,2-deoxyphosphatidylcholine as a Boundary Lipid Model" Biochim. Biophys. Acta. 1024. 209-219 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Sunamoto: "Deuterium Nuclear Magnetic Resonance Studies on the Interaction of Glycophorin with 1,2-Dimyristoylamido-1,2-deoxyphosphatidylcholine" Biochim. Biophys. Acta. 1024. 220-226 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] "Effective Transfer of Membrane Proteins from Human Erythrocytes to Artificial Boundary Lipid Containing Liposomes" Chem., Lett.1990. 1249-1252 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Sunamoto: "Structural Stability of Lecithin Liposomes as Improved by Adding an Artificial Boundary Lipid" Chem. Lett.1990. 2141-2144 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Sunamoto: "Effective Transfer of Membrane Proteins from Intact Cells to Liposomes and Preparation of Liposomal Vaccines" Ann. N. Y. Acad. Sci.(Proceedings of the 10th EnzymeEngineering Conference). 613. 116-127 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R. Shibata: "Induction of in vitro and in vivo Anti-Tumor Responses by Sensitization of Mice with Liposomes Containing a Crude Butanol Extract of Leukemia Cells and Transferred Intermembranously with Cell Surface Proteins" Int. J. Cancer. 48. 434-442 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Sunamoto: "Direct Transfer of Tumor Surface Antigen-Presenting Protein (TSAP) from Tumor Cell to Liposome for Making Liposomal Vaccine" J. Controlled Release.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Sunamoto: "Synthesis and Characterization of 1,2ーDimyristoylamidoー1,2ーdeoxyphosphatidylcholine as a Boundary Lipid Model." Biochim.Biophys.Acta. 1024. 209-219 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Sunamoto: "Deuterium Nuclear Magnetic Resonance Studies on the Interaction of Glycophorin with 1,2ーDimyristoylamidoー1,2ーdeoxyphosphatidylcholine" Biochim.Biophys.Acta. 1024. 220-226 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Sunamoto: "Effective Transfer of Membrane Proteins from Human Erythrocytes to Artificial Boundary Lipid Containing Liposomes" Chem.Lett.1990. 1249-1252 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Sunamoto: "Structual Stability of Lecithin Liposomes as Improved by Adding an Artificial Boundary Lipid," Chem.Lett.1990. 2141-2144 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Sunamoto: "Effective Transfer of Membrane Proteins from Intact Cells to Liposomes and Preparation of Liposomal Vaccines" Ann.N.Y.Acad.Sci.(Proceedings of the 10th Enzyme Engineering Conference). 613. 116-127 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] R.Shibata: "Induction of in vitro and in vivo AntiーTumor Responses by Sensitization of Mice with Liposomes Containing a Crude Butanol Extract of Leukemia Cells and Transferred Intermembranously with Cell Surface Proteins" Int.J.Cancer. 48. 434-442 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Sunamoto: "Direct Transfer of Tumor Surface AntigenーPresenting Protein(TSAP) from Tumor Cell to Liposome for Making Liposomal Vaccine" J.Controlled Release.

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Sunamoto: "Effective Transfer of Membrane Proteins from Human Erythrocytes to Artificial Boundary Lipid Containing Liposomes" Chem.Lett.1990. 1249-1252 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Sunamoto: "Sythesis and Characterization of 1,2ーDimyrixtoylamidoー1, 2ーdeoxyphosphatidylcholine as a Boundary Lipid Model." Biochim.Biophys.Acta. 1024. 209-219 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Sunamoto: "Direct Transter of Tumor Surface AntigenーPresenting Protain(TSAP) from Tumor Cell to Liposome for Making Liposomal Vaccine" J.Controlled Release.

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Sunamoto: "Effective Transfer of Membrane Proteins from Intact Cells to Liposomes and Preparation of Liposomal Vaccines" Proceeding of the 10th Enzyrme Engineering Conference.

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Shibata: "Induction of in vitro and in vivo AntiーTumor Responses by Sensitization of Mice with Liposomes Containing a Crude Butanol Extract of Leukemia Cells and Transferred Intermembranously with Cell Surface Protains" Int.J.Cancer.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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