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Research on the Effect of Light Irradiation on Metabolite Production using Cultured Plant Cell

Research Project

Project/Area Number 02453115
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 反応工学
Research InstitutionThe University of Tokyo

Principal Investigator

FURUSAKI Shintaro  Univ. of Tokyo, Dept. Chem. Eng., Professor, 工学部, 教授 (40011209)

Co-Investigator(Kenkyū-buntansha) SEKI Minoru  Univ. of Tokyo, Dept. Chem. Eng., Res. Associate, 工学部, 助手 (80206622)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsPlant Cell / Bioreactor / Alkaloid / Light Irradiation / Coffee / Caffeine / Immobilization / ベニバナ / ビタミンE
Research Abstract

1) Effect of light irradiation on the secondary-metabolite production of coffee plant cells
The effect of light on caffeine production by Coffea arabica cells was investigated using chemical actinometry for the purpose of industrial application of plant cell cultures. Caffeine production by the Coffea arabica cells was greedy increased by light. About a six-day irradiation period was required to increase the caffeine production in a batch culture. Caffeine production significantly increased at a low light irradiation rate of 0.11 J/L/S. Meanwhile, cell growth was suppressed by increasing the irradiation rate. It was shown there was a light irradiation rate that optimized production. Since longterm continuous light greatly suppressed cell growth, the culture was divided into two stages : the production period in the light and the growth period in the dark. The high production and good growth were maintained for 65 days.
2) Estimation of light adsorption rate by a cell.
We developed a novel method for quantitative determination of the ratio of light transmission to reflection in cell suspension using chemical actinometry, and estimated light adsorption rate by a cell, which presents useful information for design strategy of photobioreactor.
3) Construction of photo-bioreactors
We proposed the culture system with adsorption column for the product removal and presented good results. Immobilized cell system using the plant cell in a bubble column reactor achieved higher productivity and cell growth.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] H.Kurata,M.Seki S.Furusaki,T.Furuya: "Influence of light irradiation rates and irradiation modes on caffeine production and cell growth in suspension culture of Coffea arabica cells." J.Chem.Eng.Japan. 24. 783-788 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kurata,M.Seki S.Furusaki,T.Furuya: "Effect of light on caffeine production by plant cells of coffea arabica." Ann.New York Acad.Sci. (Enzyme Engineering 10). 613. 538-541 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Furusaki,M.Seki H.Kurata,T.Furuya: "Proceedings of 3rd U.S./Japan Conf.on Biotechnol.(分担)" SpringerーVerlag, 10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kurata, M. Seki, S. Furusaki, and T. Furuya: "Influence of light irradiation rates and irradiation modes on caffeine production and cell growth in suspension culture of Coffea arabica cells." J. Chem. Eng. Japan. 24. 783-788 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kurata, M. Seki, S. Furusaki, and T. Furuya: "Effect of light on caffeine production by plant cells of Coffea arabica" Ann. New York Acad. Sci., 613. (Enzyme Engineering 10). 538-541 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Furusaki, M. Seki, H. Kurata, T. Furuya: "The effect of light irradiation on secondarymetabolite production by Coffea arabica cells." Proc. 3rd US/Japan Conf. on Biotechnol.Springer- Verlag, Heiderberg. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kurata,M.Seki,S.Furusaki,T.Furuya: "Influence of light irradiation rates and irradiation mades on caffeine production and cell growth in suspension culfure of Coffea arabica cells." J. Chem. Eng. Japan. 24. 783-788 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kurata,M.Seki,S.Furusaki&T.Furuya: "Effect of Light on Caffeine Production by Plant Cells of <Coffea>___ー <arabica>___ー" Ann.New York Acad.Sci.613. 538-541 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kurata,M.Seki,S.Furusaki&T.Furuya: "The influence of light on caffeine production and cell growth in suspension culture of <Coffea>___ー <arabica>___ー cells" Applied Microbiol.Biotechnol.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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