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Studies on structural alteration of high molecular biomolecules by high pressure treatment

Research Project

Project/Area Number 02453127
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionKyoto University

Principal Investigator

HAYASHI Rikimaru  KYOTO University, Research Institute for Food Science, Associate Professor, 食糧科学研究所, 助教授 (90027186)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Mieko  Home Economics, Shujitu Junior College, Associate Professor, 家政学科, 助教授 (90152355)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsHigh pressure effect / Pressure-processed food / Pressure-processing / Pressure sanitation / Food preservation / Pressure-cooking / 加圧調理
Research Abstract

High pressure effects at several thousands bars or higher on bionoleacules have not yet been analyzed, but since the proposition of the author that high pressure use in food science and technology is indispensable for the recent food industries, many studies on the high pressure effects on foods and food components have been developed not only in of food industries but also in academic and governmental institutions. In this study, the purpose is placed to study about high pressure effects on biomolecules, especially on high molecular weight compounds and cellular granules. and to obtain the information about their naturaistructure, expecting to construct a basic science of high pressure-processed foods. The results are as follows.
1. Irreversible changes of protein and starches were directly observed under high pressure and compared with heat effects. As the result, pressure effects were weaker than heat effects.
2. Using fresh tissues of animal and plant, pressure effects on cellular membranes and granules have been studied. As the result, pressure tended to destroy the complex structure of protein and lipid more easily than protein and starch molecules. Starch granules were resistant against pressure treatment.
3. Chemical modification of S-S bridges of several proteins under high pressure revealed unique features about their tertiary structure.
4. Investigations of relationships of pressure and heat effects on 20 kinds of starch granule resulted in the classification of A and B types of starches. Retrogradation of pressure-treated starches was slower than heat-treated ones.
5. Pressure effects on chemical reactions under high temperature and high pressure was analyzed.
These results provided the scientific basis of processing, preservation, and sanitation of foods.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] 岡本 巳恵子,林 力丸: "Application of high pressure to food processing:Textural comparison of pressure^ー and heatーinduced gels of food proteins" Agric.Biol.Chem.54. 183-189 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 原 昭弘,林 力丸: "生酒に含まれる各種酵素および微生物の加圧失活" 農化誌. 64. 1025-1030 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松本 正,林 力丸: "大豆タンパク質の高圧処理による加工特性" 農化誌. 64. 1455-1459 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大森 丘,林 力丸: "Effect of high pressure on the protease activities in meat" Agric.Biol.Chem.55. 357-361 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 重久 保,林 力丸: "Effect of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products" Int.J.Food Microbiol.12. 207-216 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岡本 巳恵子,林 力丸: "High pressure use for proteolytic digstion of food proteins:The selective elimination of βーlactoglobulin in bovine milk whey concentrate" Agric.Biol.Chem.55. 1253-1257 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 玉岡 徹,林 力丸: "High pressure effects on Maillard reaction" Agric.Biol.Chem.55. 2071-2074 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 朝賀 昌志,林 力丸: "Activatioin of polyphenoloxidase in pear fruits by high pressure treatment" Agric.Biol.Chem.55. 2439-2440 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸: "High pressure in food processing and preservation:Principle,application and development" High Pressure Research. 7. 15-21 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸: "加圧調理ー原理と特徴" 調理科学. 24. 260-265 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸: "食品への高圧利用ー加圧食品の物性" 熱物性. 5. 282-290 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸: "食品の加工と保蔵への高圧利用ー特に低温加圧の利用を中心に" 日本低温保蔵学会誌. 17. 23-30 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸(編著): "加圧食品ー研究と開発" さんえい出版, 375 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸(編著): "高圧科学と加圧食品" さんえい出版, 460 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸: "酵母研究技法の新展開" 学会出版センタ-, 262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 林 力丸: "高圧流体技術" 丸善株式会社, 354 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Okamoto, R. Hayashi: "Application of high pressure to food processing : Textural comparison of pressure- and heat-induced gels of food proteins" Agric. Biol. Chem.54. 183-189 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. Hara, R. Hayashi: "Effects of high pressure on inactivation of enzymes and microorganisms in non-pasteurized rice wine (Namazake)" Nippon Nogeikagaku Kaishi. 64. 1025-1030 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Matsumoto, R. Hayashi: "Properties of Pressure-induced gels of various soy protein products" Nippon Nogeikagaku Kaishi. 64. 1455-1459 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Oomori, R. Hayashi: "Effect of high pressure on the protease activities in meat" Agric. Biol. Chem.55. 357-361 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Shigehisa, R. Hayashi: "Effect of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products" Int. J. Food Microbiol.12. 207-216 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Okamoto, R. Hayashi: "High pressure use for proteolytic digestion of food proteins : The selective elimination of beta-lactoglobulin in bovine milk whey concentrate" Agric. Biol. Chem.55. 1253-1257 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Tamaoka, R. Hayashi: "High pressure effects on Maillard reaction" Agric. Biol. Chem.55. 2071-2074 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Asaka, R. Hayashi: "Activatioin of polyphenoloxidase in pear fruits by high pressure treatment" Agric. Biol. Chem.55. 2439-2440 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R. Hayashi: "High pressure in food processing and preservation : Principle, application and development" High Pressure Res.7. 15-21 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R. Hayashi (Ed.): San-Ei Shuppan Co.Pressure-Processed Foods-Research and Development, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R. Hayashi (Ed.): San-Ei Shuppan Co.High Pressure Science and Pressure-Processed Foods, (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大森 丘,林 力丸: "Effect of high pressure on the protease activities in meat" Agric.Biol.Chem.55. 357-361 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 重久 保,林 力丸: "Effect of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products" Int.J.Food Microbiol.12. 207-216 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岡本 巳恵子,林 力丸: "High pressure use for proteolytic digestion of food proteins:The selective elimination of βーlactoglobulin in bovine milk whey concentrate" Agric.Biol.Chem.55. 1253-1257 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 玉岡 徹,林 力丸: "High pressure effects on Maillard reaction" Agric.Biol.Chem.55. 2071-2074 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 朝賀 昌志,林 力丸: "Activatioin of polyphenoloxidase in pear fruits by high pressure treatmnt" Agric.Biol.Chem.55. 2439-2440 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 林 力丸: "High pressure in food processing and preservation:Principle,application and development" High Pressure Research. 7. 15-21 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 林 力丸(編著): "高圧科学と加圧食品" さいえん出版, 460 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 林 力丸: "高圧流体技術" 丸善株式会社, 354 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 原 昭弘: "生酒に含まれる各種酵素および微生物の加圧失活" 農化誌. 64. 1025-1029 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 松本 正: "大豆タンパク質の高圧による加工特性" 農化誌. 64. 1455-1459 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 林 力丸: "Preparation of amidated gelatins and their physicochemical properties" Agric.Biol.Chem.54. 2213-2218 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大森 丘: "Effect of high pressure on the protease activities in meat" Agric.Biol.Chem.55. 357-361 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 重久 保: "Effect of high hydrostatic pressure on chacteristics of pork slurries and inactivation of microorganisms associated with meat and meat products" Int.J.Food Microbiol.

    • Related Report
      1990 Annual Research Report
  • [Publications] 岡本 巳恵子: "High pressure use for proteolytic digestion of food proteins:The selective elimination of βーlactoglobulin in bovine milk whey concentrate" Agric.Biol.Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] 林 力丸: "90年代の食品加工技術" シ-エムシ-, 302 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 林 力丸: "加圧食品ー研究と開発" さんえい出版, 374 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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